Evaluation of a flow cytometry method for CD4 T cell enumeration based on volumetric primary CD4 gating using thermoresistant reagents

• Published in: Journal of immunological methods Vol. 372; no. 1; pp. 7 - 13
• Main Authors: Dieye, Tandakha Ndiaye, Diaw, Papa Alassane, Daneau, Géraldine, Wade, Djibril, Sylla Niang, Maguette, Camara, Makhtar, Diallo, Abdoul Aziz, Toure Kane, Coumba, Diop Ndiaye, Halimatou, Mbengue, Babacar, Dieye, Alioune, Kestens, Luc, Mboup, Souleymane
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 30.09.2011; Elsevier
• Subjects: Biological and medical sciences; CD4 counts; CD4 Lymphocyte Count - methods; CD4 Lymphocyte Count - standards; CD4-positive T-lymphocytes; CD4-Positive T-Lymphocytes - cytology; CD4-Positive T-Lymphocytes - immunology; CD4-Positive T-Lymphocytes - virology; correlation; Flow cytometry; Flow Cytometry - instrumentation; Flow Cytometry - methods; Flow Cytometry - standards; Fundamental and applied biological sciences. Psychology; Fundamental immunology; HIV; HIV - immunology; HIV Infections - blood; HIV Infections - immunology; HIV Infections - virology; Human immunodeficiency virus; Humans; Indicators and Reagents - standards; Italy; Microbiology; Molecular immunology; monitoring; patients; Primary gating; Regression Analysis; Reproducibility of Results; Resource-limited; Senegal; Techniques; Techniques used in virology; United States; Virology; Volumetric; United States; Italy; Senegal; CHUN Fann; Flow cytometry; ART; Volumetric; HIV; IHS; Resource-limited; CD4 counts; DGDC; Primary gating; CD4 T lymphocyte; Retroviridae; Lentivirus; Enumeration(counting); Immunological method; Virus; Human immunodeficiency virus

Laboratory follow-up of HIV patients in resource-limited settings requires appropriate instruments for CD4 T cell enumeration. In this study, we evaluated the application of a simplified, mobile and robust flow cytometry system, the Apogee Auto 40 analyzer (Auto40) using thermoresistant reagents, fo...