Oxidative Stress Protection by Exogenous Delivery of rhHsp70 Chaperone to the Retinal Pigment Epithelium (RPE), a Possible Therapeutic Strategy Against RPE Degeneration

• Published in: Pharmaceutical research Vol. 32; no. 1; pp. 211 - 221
• Main Authors: Subrizi, Astrid, Toropainen, Elisa, Ramsay, Eva, Airaksinen, Anu J., Kaarniranta, Kai, Urtti, Arto
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.01.2015; Springer Nature B.V
• Subjects: Animals; Biochemistry; Biomedical and Life Sciences; Biomedical Engineering and Bioengineering; Biomedicine; Cell Culture Techniques; Cell Line; Cell Survival - drug effects; Cellular biology; Heat shock proteins; HSP70 Heat-Shock Proteins - administration & dosage; HSP70 Heat-Shock Proteins - pharmacokinetics; HSP70 Heat-Shock Proteins - therapeutic use; Humans; Hydrogen Peroxide - toxicity; Interleukin-6 - secretion; Intravitreal Injections; Macular Degeneration - immunology; Macular Degeneration - metabolism; Macular Degeneration - pathology; Macular Degeneration - prevention & control; Medical Law; Oxidative stress; Oxidative Stress - drug effects; Pharmaceutical sciences; Pharmacology/Toxicology; Pharmacy; Recombinant Proteins; Research Paper; Retina; Retinal Pigment Epithelium - drug effects; Retinal Pigment Epithelium - immunology; Retinal Pigment Epithelium - metabolism; Retinal Pigment Epithelium - pathology; Swine; Tissue Distribution; AMD; Protein delivery; Oxidative stress; Hsp70; RPE

Purpose To measure the cytoprotective effects of rhHsp70 against oxidative stress and study its cellular uptake, intracellular and intraocular distribution in the retinal pigment epithelium. Methods Human retinal pigment epithelial cells (ARPE-19) were pre-treated with rhHsp70 for 24 h, 48 h, and 72...