Intracellular detection of differential APOBEC3G, TRIM5alpha, and LEDGF/p75 protein expression in peripheral blood by flow cytometry - Mous, Kim; Jennes, Wim; De Roo, Ann; Pintelon, Isabel; Kestens, Luc; Van Ostade, Xaveer (Journal of immunological methods, 2011)
Keywords: Antibodies; APOBEC-3G Deaminase; APOBEC3G; apolipoprotein B; Biological and medical sciences; blood flow; Carrier Proteins - biosynthesis; Carrier Proteins - genetics; Carrier Proteins - immunology; CD4-positive T-lymphocytes; Cytidine Deaminase - biosynthesis; Cytidine Deaminase - genetics; Cytidine Deaminase - immunology; cytoplasm; enzyme-linked immunosorbent assay; flow cytometry; Flow Cytometry - methods; fluorescence; Fundamental and applied biological sciences. Psychology; Fundamental immunology; HIV infections; HIV Infections - blood; HIV Infections - virology; HIV-1 - genetics; HIV-1 - metabolism; HIV-1 - physiology; Human immunodeficiency virus 1; Humans; image analysis; Intercellular Signaling Peptides and Proteins - biosynthesis; Intercellular Signaling Peptides and Proteins - genetics; Intercellular Signaling Peptides and Proteins - immunology; interferon-alpha; interferon-beta; Intracellular staining; LEDGF/p75; Leukocytes, Mononuclear - immunology; microscopy; Molecular immunology; monocytes; patients; Protein expression; protein synthesis; quantitative polymerase chain reaction; recombinant proteins; Reproducibility of Results; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; Statistics, Nonparametric; Techniques; TRIM5alpha; Virus Replication; Western blotting; Protein expression; Intracellular staining; LEDGF/p75; APOBEC3G; TRIM5alpha; Flow cytometry; Peripheral blood circulation; Intracellular; Detection; Protein; Immunological method