Lack of detection of a putative retrovirus associated with haemic neoplasia in the soft shell clam Mya arenaria

• Published in: Journal of invertebrate pathology Vol. 109; no. 1; pp. 97 - 104
• Main Authors: AboElkhair, M., Iwamoto, T., Clark, K.F., McKenna, P., Siah, A., Greenwood, S.J., Berthe, F.C.J., Casey, J.W., Cepica, A.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.01.2012; Elsevier
• Subjects: Animals; Biological and medical sciences; centrifugation; Centrifugation, Density Gradient - methods; clams; disease transmission; electron microscopy; electrophoresis; etiology; filtrates; Fundamental and applied biological sciences. Psychology; genes; Haemic neoplasia; Hematologic Neoplasms - pathology; Hematologic Neoplasms - veterinary; Hematologic Neoplasms - virology; Hemocytes - ultrastructure; Hemocytes - virology; Invertebrates; Marine; Mollusca; Mya - virology; Mya arenaria; Retroviridae; Retroviridae - enzymology; Retroviridae - genetics; Retroviridae - isolation & purification; Retroviridae Infections - diagnosis; Retroviridae Infections - veterinary; Retroviridae Infections - virology; Retrovirus; Reverse transcriptase activity; reverse transcriptase polymerase chain reaction; Reverse Transcriptase Polymerase Chain Reaction - veterinary; RNA; RNA, Viral - analysis; RNA, Viral - genetics; RNA-directed DNA polymerase; RNA-Directed DNA Polymerase - genetics; RNA-Directed DNA Polymerase - metabolism; RT-PCR; shell (molluscs); sucrose; Sucrose gradient; tissues; viruses; Mya arenaria; Retrovirus; Haemic neoplasia; Sucrose gradient; Reverse transcriptase activity; RT-PCR; Reverse transcription; Activity; Bivalvia; Invertebrata; Mollusca; Detection; Reverse transcription polymerase chain reaction

[Display omitted] ► Whole tissues of both HN+ve and HN−ve animals were exposed to sucrose gradient. ► The fractions with highest RT activity were exposed to RNA, protein, and EM analysis. ► There was no evidence for existing of a putative retrovirus. ► RT-PCR using universal primers of retroviruses...