Branched-DNA Assay for Detection of the mecA Gene in Oxacillin-Resistant and Oxacillin-Sensitive Staphylococci

• Published in: Journal of clinical microbiology Vol. 36; no. 9; pp. 2640 - 2644
• Main Authors: Kolbert, C. P., Arruda, J., Varga-Delmore, P., Zheng, X., Lewis, M., Kolberg, J., Persing, D. H.
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.09.1998
• Subjects: Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; DNA Primers; DNA, Bacterial - genetics; Fundamental and applied biological sciences. Psychology; Genes, Bacterial; Humans; Methicillin Resistance - genetics; Microbiology; Nucleic Acid Hybridization - methods; Oxacillin - pharmacology; Phenotype; Staphylococcal Infections - microbiology; Staphylococcus - genetics; Staphylococcus - growth & development; Staphylococcus - isolation & purification; Method; Staphylococcus; Polymerase chain reaction; Resistance; Branched chain; Antibiotic; Oxacillin; DNA; Bacteria; Micrococcales; Micrococcaceae; Antibacterial agent; Detection

The identification of methicillin-resistant staphylococcus isolates in the clinical laboratory has typically been performed by using methods that detect phenotypic expression of resistance determinants. However, these methods may be difficult to interpret and some isolates do not express resistance...