RT‐PCR/MALDI‐TOF mass spectrometry‐based detection of SARS‐CoV‐2 in saliva specimens

• Published in: Journal of medical virology Vol. 93; no. 9; pp. 5481 - 5486
• Main Authors: Hernandez, Matthew M., Banu, Radhika, Shrestha, Paras, Patel, Armi, Chen, Feng, Cao, Liyong, Fabre, Shelcie, Tan, Jessica, Lopez, Heidi, Chiu, Numthip, Shifrin, Biana, Zapolskaya, Inessa, Flores, Vanessa, Lee, Pui Yiu, Castañeda, Sergio, Ramírez, Juan David, Jhang, Jeffrey, Osorio, Giuliana, Gitman, Melissa R., Nowak, Michael D., Reich, David L., Cordon‐Cardo, Carlos, Sordillo, Emilia Mia, Paniz‐Mondolfi, Alberto E.
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.09.2021; John Wiley and Sons Inc
• Subjects: Assaying; Benchmarking; Coronaviruses; COVID-19 - diagnosis; COVID-19 - virology; COVID-19 Nucleic Acid Testing - instrumentation; COVID-19 Nucleic Acid Testing - methods; COVID-19 Nucleic Acid Testing - standards; Diagnostic systems; Diagnostic Tests, Routine - instrumentation; Diagnostic Tests, Routine - methods; Diagnostic Tests, Routine - standards; Humans; Limit of Detection; MALDI‐TOF; Mass spectrometry; Mass spectroscopy; Mathematical analysis; Nasopharynx - virology; Performance evaluation; real‐time RT‐PCR; Respiratory diseases; RNA, Viral - genetics; Saliva; Saliva - virology; SARS-CoV-2 - genetics; SARS‐CoV‐2; Scientific imaging; Sensitivity analysis; Severe acute respiratory syndrome; Severe acute respiratory syndrome coronavirus 2; Specimen Handling - standards; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - standards; Spectroscopy; Virology; saliva; SARS-CoV-2; MALDI-TOF; real-time RT-PCR

As severe acute respiratory syndrome coronavirus‐2 (SARS‐CoV‐2) infections continue, there is a substantial need for cost‐effective and large‐scale testing that utilizes specimens that can be readily collected from both symptomatic and asymptomatic individuals in various community settings. Although...