Analysis of CD8 T cell reactivity to cytomegalovirus using protein‐spanning pools of overlapping pentadecapeptides

• Published in: European journal of immunology Vol. 30; no. 6; pp. 1676 - 1682
• Main Authors: Kern, Florian, Faulhaber, Nicole, Frömmel, Claudia, Khatamzas, Elham, Prösch, Susanna, Schönemann, Constanze, Kretzschmar, Ines, Volkmer‐Engert, Rudolf, Volk, Hans‐Dieter, Reinke, Petra
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY‐VCH Verlag GmbH 01.06.2000
• Subjects: AIDS/HIV; AIE1 protein; Amino Acid Sequence; CD8 antigen; CD8-Positive T-Lymphocytes - immunology; Cytomegalovirus - immunology; Cytomegalovirus Infections - blood; Cytomegalovirus Infections - immunology; g-Interferon; HLA-A1 Antigen - immunology; HLA-A2 Antigen - immunology; HLA-B7 Antigen - immunology; HLA-B8 Antigen - immunology; Human cytomegalovirus; Humans; IE1 protein; Immediate-Early Proteins - immunology; Immediate‐early‐1 protein; Interferon-gamma - biosynthesis; Molecular Sequence Data; pentadecapeptides; Peptide pool; Peptides - immunology; Phosphoproteins - immunology; pp65; Viral Matrix Proteins - immunology; Viral Proteins

The frequencies of human cytomegalovirus (HCMV) protein‐specific CD8 T cells, identified by the presence of intracellular IFN‐γ, were measured by flow cytometry following stimulation of freshly isolated peripheral blood mononuclear cells (PBMC) with comprehensive peptide pools. These pools spanned t...