An evolving approach to the laboratory assessment of COVID‐19

As the 2019 novel coronavirus disease (COVID‐19) outbreak has evolved in each country, the approach to the laboratory assessment of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection has had to evolve as well. This review addresses the evolving approach to the laboratory assessme...

Full description

Saved in:
Bibliographic Details
Published inJournal of medical virology Vol. 92; no. 10; pp. 1812 - 1817
Main Authors Lu, Hongzhou, Stratton, Charles W., Tang, Yi‐Wei
Format Journal Article
LanguageEnglish
Published United States Wiley Subscription Services, Inc 01.10.2020
John Wiley and Sons Inc
Subjects
Algorithms
Antibodies, Viral - immunology
assessment
China
Coronaviridae
Coronaviruses
COVID-19
COVID-19 - diagnosis
COVID-19 - immunology
COVID-19 - virology
Evolution
Humans
Immunoassay
Laboratories
molecular testing
Outbreaks
Pandemics - prevention & control
Pneumonia, Viral - diagnosis
Pneumonia, Viral - immunology
Pneumonia, Viral - virology
Review
Reviews
SARS-CoV-2 - immunology
Serologic Tests - methods
Serology
Severe acute respiratory syndrome
Severe acute respiratory syndrome coronavirus 2
Viral diseases
Virology
China
COVID-19
assessment
molecular testing
serology
Online AccessGet full text

Cover

Abstract As the 2019 novel coronavirus disease (COVID‐19) outbreak has evolved in each country, the approach to the laboratory assessment of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection has had to evolve as well. This review addresses the evolving approach to the laboratory assessment of COVID‐19 and discusses how algorithms for testing have been driven, in part, by the demand for testing overwhelming the capacity to accomplish such testing. This review focused on testing in the USA, as this testing is evolving, whereas in China and other countries such as South Korea testing is widely available and includes both molecular testing for SARS‐CoV‐2 as well as serological testing using both enzyme‐linked immunosorbent assay methodology and lateral flow immunoassay methodology. Although commercial testing systems are becoming available, there will likely be insufficient numbers of such tests due to high demand. Serological testing will be the next testing issue as the COVID‐19 begins to subside. This will allow immunity testing as well as will allow the parameters of the COVID‐19 outbreak to be defined.
AbstractList As the 2019 novel coronavirus disease (COVID‐19) outbreak has evolved in each country, the approach to the laboratory assessment of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection has had to evolve as well. This review addresses the evolving approach to the laboratory assessment of COVID‐19 and discusses how algorithms for testing have been driven, in part, by the demand for testing overwhelming the capacity to accomplish such testing. This review focused on testing in the USA, as this testing is evolving, whereas in China and other countries such as South Korea testing is widely available and includes both molecular testing for SARS‐CoV‐2 as well as serological testing using both enzyme‐linked immunosorbent assay methodology and lateral flow immunoassay methodology. Although commercial testing systems are becoming available, there will likely be insufficient numbers of such tests due to high demand. Serological testing will be the next testing issue as the COVID‐19 begins to subside. This will allow immunity testing as well as will allow the parameters of the COVID‐19 outbreak to be defined.
As the 2019 novel coronavirus disease (COVID-19) outbreak has evolved in each country, the approach to the laboratory assessment of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has had to evolve as well. This review addresses the evolving approach to the laboratory assessment of COVID-19 and discusses how algorithms for testing have been driven, in part, by the demand for testing overwhelming the capacity to accomplish such testing. This review focused on testing in the USA, as this testing is evolving, whereas in China and other countries such as South Korea testing is widely available and includes both molecular testing for SARS-CoV-2 as well as serological testing using both enzyme-linked immunosorbent assay methodology and lateral flow immunoassay methodology. Although commercial testing systems are becoming available, there will likely be insufficient numbers of such tests due to high demand. Serological testing will be the next testing issue as the COVID-19 begins to subside. This will allow immunity testing as well as will allow the parameters of the COVID-19 outbreak to be defined.As the 2019 novel coronavirus disease (COVID-19) outbreak has evolved in each country, the approach to the laboratory assessment of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has had to evolve as well. This review addresses the evolving approach to the laboratory assessment of COVID-19 and discusses how algorithms for testing have been driven, in part, by the demand for testing overwhelming the capacity to accomplish such testing. This review focused on testing in the USA, as this testing is evolving, whereas in China and other countries such as South Korea testing is widely available and includes both molecular testing for SARS-CoV-2 as well as serological testing using both enzyme-linked immunosorbent assay methodology and lateral flow immunoassay methodology. Although commercial testing systems are becoming available, there will likely be insufficient numbers of such tests due to high demand. Serological testing will be the next testing issue as the COVID-19 begins to subside. This will allow immunity testing as well as will allow the parameters of the COVID-19 outbreak to be defined.
Author Lu, Hongzhou
Tang, Yi‐Wei
Stratton, Charles W.
AuthorAffiliation 2 Department of Pathology, Microbiology, and Immunology Vanderbilt University Medical Center Nashville Tennessee
1 Shanghai Public Health Clinical Center Fudan University Shanghai China
3 Cepheid, Danaher Diagnostic Platform Shanghai China
AuthorAffiliation_xml – name: 3 Cepheid, Danaher Diagnostic Platform Shanghai China
– name: 2 Department of Pathology, Microbiology, and Immunology Vanderbilt University Medical Center Nashville Tennessee
– name: 1 Shanghai Public Health Clinical Center Fudan University Shanghai China
Author_xml – sequence: 1
  givenname: Hongzhou
  surname: Lu
  fullname: Lu, Hongzhou
  organization: Fudan University
– sequence: 2
  givenname: Charles W.
  orcidid: 0000-0002-3630-0537
  surname: Stratton
  fullname: Stratton, Charles W.
  organization: Vanderbilt University Medical Center
– sequence: 3
  givenname: Yi‐Wei
  orcidid: 0000-0003-4888-6771
  surname: Tang
  fullname: Tang, Yi‐Wei
  email: yi-wei.tang@cepheid.com
  organization: Cepheid, Danaher Diagnostic Platform
BackLink https://www.ncbi.nlm.nih.gov/pubmed/32347966$$D View this record in MEDLINE/PubMed
BookMark eNp1kb1OwzAUhS0EghYYeAEUiQWGgP9i1wuoKv8qYoGulmMc6iqJi50WdeMReEaeBJcWBAh0Bw_3O0fn-rTBau1qA8AOgocIQnw0qqaHOBMZXQEtBAVLBeRoFbQgoixlDGUboB3CCELYERivgw2CCeWCsRY46daJmbpyauvHRI3H3ik9TBqXNEOTlCp3XjXOzxIVggmhMnWTuCLp3Q6uTt9eXpHYAmuFKoPZXr6b4P787K53mfZvL6563X6qKUc0VcgUGRZKMGpMLjRGOc8wopgrgbTKc0yR1lSTDmUF45rmBOYFp4XhRZQisgmOF77jSV6ZBx2DeFXKsbeV8jPplJU_N7Udykc3lRwzjgWOBvtLA--eJiY0srJBm7JUtXGTIDERjEBKRCeie7_QkZv4Op4nMaUwDstIpHa_J_qK8vm3EThaANq7ELwppLaNaqybB7SlRFDO25OxPfnRXlQc_FJ8mv7FLt2fbWlm_4Py-mawULwDZrmpYA
CitedBy_id crossref_primary_10_1080_22221751_2020_1825016
crossref_primary_10_1016_j_aca_2023_341931
crossref_primary_10_1002_jmv_26809
crossref_primary_10_3390_v13010040
crossref_primary_10_46332_aemj_1061193
crossref_primary_10_1097_MCP_0000000000000768
crossref_primary_10_18821_0869_2084_2020_65_12_757_766
crossref_primary_10_1038_s41467_022_31300_9
crossref_primary_10_3390_jpm11010042
crossref_primary_10_1007_s12070_021_02849_6
crossref_primary_10_1016_j_jcv_2020_104568
crossref_primary_10_1039_D1AN00304F
crossref_primary_10_56085_01238590_217
crossref_primary_10_13005_bbra_2851
crossref_primary_10_3904_kjim_2020_257
Cites_doi 10.1371/journal.pone.0021610
10.1128/JCM.06186-11
10.1093/cid/ciaa149
10.1128/JCM.01691-17
10.1128/JCM.01324-18
10.1016/j.jviromet.2017.09.020
10.1016/j.jaut.2020.102433
10.1016/j.virusres.2014.03.018
10.3390/microarrays1030107
10.1080/22221751.2020.1743767
10.1056/NEJMp2005118
10.1128/JCM.00930-18
10.1128/JCM.00590-20
10.1080/22221751.2020.1745095
10.1128/JCM.00512-20
10.1371/journal.pone.0211079
10.1128/JCM.01164-09
10.1016/S0140-6736(20)30788-1
10.1042/EBC20150012
10.1016/S0140-6736(20)30673-5
10.1128/JCM.06551-11
10.1016/bs.aivir.2019.08.002
10.1016/j.jcv.2017.09.007
10.1016/j.jviromet.2015.07.002
10.1056/NEJMe2009651
10.1002/jmv.25678
10.7326/L20-0175
10.1080/22221751.2020.1729071
10.1128/JCM.00482-16
10.1007/s11427-020-1661-4
10.1128/mBio.00722-20
10.1016/j.jviromet.2004.03.015
10.1002/jmv.25800
10.1038/s41586-020-2196-x
10.1093/cid/ciaa344
10.3201/eid2607.200841
10.2807/1560-7917.ES.2020.25.9.2000152
10.1038/emi.2017.35
ContentType Journal Article
Copyright 2020 Wiley Periodicals LLC
2020 Wiley Periodicals LLC.
Copyright_xml – notice: 2020 Wiley Periodicals LLC
– notice: 2020 Wiley Periodicals LLC.
DBID AAYXX
CITATION
CGR
CUY
CVF
ECM
EIF
NPM
7QL
7TK
7U9
8FD
C1K
FR3
H94
K9.
M7N
P64
RC3
7X8
5PM
DOI 10.1002/jmv.25954
DatabaseName CrossRef
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
Bacteriology Abstracts (Microbiology B)
Neurosciences Abstracts
Virology and AIDS Abstracts
Technology Research Database
Environmental Sciences and Pollution Management
Engineering Research Database
AIDS and Cancer Research Abstracts
ProQuest Health & Medical Complete (Alumni)
Algology Mycology and Protozoology Abstracts (Microbiology C)
Biotechnology and BioEngineering Abstracts
Genetics Abstracts
MEDLINE - Academic
PubMed Central (Full Participant titles)
DatabaseTitle CrossRef
MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
Genetics Abstracts
Virology and AIDS Abstracts
Technology Research Database
Bacteriology Abstracts (Microbiology B)
Algology Mycology and Protozoology Abstracts (Microbiology C)
AIDS and Cancer Research Abstracts
ProQuest Health & Medical Complete (Alumni)
Engineering Research Database
Neurosciences Abstracts
Biotechnology and BioEngineering Abstracts
Environmental Sciences and Pollution Management
MEDLINE - Academic
DatabaseTitleList
Genetics Abstracts
MEDLINE

MEDLINE - Academic
CrossRef
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Medicine
DocumentTitleAlternate LU et al
EISSN 1096-9071
EndPage 1817
ExternalDocumentID PMC7267292
32347966
10_1002_jmv_25954
JMV25954
Genre reviewArticle
Journal Article
Review
GeographicLocations China
GeographicLocations_xml – name: China
GroupedDBID ---
.3N
.55
.GA
.GJ
.Y3
05W
0R~
10A
1L6
1OB
1OC
1ZS
31~
33P
3O-
3SF
3WU
4.4
50Y
50Z
51W
51X
52M
52N
52O
52P
52R
52S
52T
52U
52V
52W
52X
53G
5GY
5RE
5VS
66C
702
7PT
8-0
8-1
8-3
8-4
8-5
8UM
930
A01
A03
AAESR
AAEVG
AAHHS
AAHQN
AAIPD
AAMNL
AANHP
AANLZ
AAONW
AASGY
AAXRX
AAYCA
AAZKR
ABCQN
ABCUV
ABEML
ABIJN
ABJNI
ABOCM
ABPVW
ABQWH
ABXGK
ACAHQ
ACBWZ
ACCFJ
ACCZN
ACGFS
ACGOF
ACMXC
ACPOU
ACPRK
ACRPL
ACSCC
ACXBN
ACXQS
ACYXJ
ADBBV
ADBTR
ADEOM
ADIZJ
ADKYN
ADMGS
ADNMO
ADOZA
ADXAS
ADZMN
AEEZP
AEIGN
AEIMD
AENEX
AEQDE
AEUQT
AEUYR
AFBPY
AFFNX
AFFPM
AFGKR
AFPWT
AFRAH
AFWVQ
AFZJQ
AHBTC
AHMBA
AI.
AIACR
AITYG
AIURR
AIWBW
AJBDE
ALAGY
ALMA_UNASSIGNED_HOLDINGS
ALUQN
ALVPJ
AMBMR
AMYDB
ASPBG
ATUGU
AVWKF
AZBYB
AZFZN
AZVAB
BAFTC
BDRZF
BFHJK
BHBCM
BMXJE
BROTX
BRXPI
BY8
C45
CS3
D-6
D-7
D-E
D-F
DCZOG
DPXWK
DR2
DRFUL
DRMAN
DRSTM
DU5
EBD
EBS
ECGQY
EJD
ELTNK
EMOBN
F00
F01
F04
F5P
FEDTE
FUBAC
G-S
G.N
GNP
GODZA
H.X
HBH
HF~
HGLYW
HHY
HHZ
HVGLF
HZ~
IX1
J0M
JPC
KBYEO
KQQ
L7B
LATKE
LAW
LC2
LC3
LEEKS
LH4
LITHE
LOXES
LP6
LP7
LUTES
LW6
LYRES
M65
MEWTI
MK4
MRFUL
MRMAN
MRSTM
MSFUL
MSMAN
MSSTM
MXFUL
MXMAN
MXSTM
N04
N05
N9A
NF~
NNB
O66
O9-
OIG
OVD
P2P
P2W
P2X
P2Z
P4B
P4D
PALCI
PQQKQ
Q.N
Q11
QB0
QRW
R.K
RGB
RIWAO
RJQFR
ROL
RWI
RX1
RYL
SAMSI
SUPJJ
SV3
TEORI
TUS
UB1
V2E
VH1
W8V
W99
WBKPD
WHG
WIB
WIH
WIJ
WIK
WJL
WNSPC
WOHZO
WQJ
WRC
WUP
WXI
WXSBR
WYISQ
X7M
XG1
XPP
XV2
ZGI
ZXP
ZZTAW
~IA
~KM
~WT
AAYXX
AEYWJ
AGHNM
AGQPQ
AGYGG
CITATION
CGR
CUY
CVF
ECM
EIF
NPM
7QL
7TK
7U9
8FD
AAMMB
AEFGJ
AGXDD
AIDQK
AIDYY
C1K
FR3
H94
K9.
M7N
P64
RC3
7X8
5PM
ID FETCH-LOGICAL-c4714-a1ef529a964eeb9c21b7521427a91cabb241cc4c3846f67c4b30bf74fe7f1ef13
IEDL.DBID DR2
ISSN 0146-6615
1096-9071
IngestDate Thu Aug 21 14:14:59 EDT 2025
Fri Jul 11 16:38:09 EDT 2025
Sat Jul 26 02:22:09 EDT 2025
Wed Feb 19 02:29:06 EST 2025
Tue Jul 01 02:24:44 EDT 2025
Thu Apr 24 23:10:56 EDT 2025
Wed Jan 22 16:32:19 EST 2025
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 10
Keywords COVID-19
assessment
molecular testing
serology
Language English
License 2020 Wiley Periodicals LLC.
This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c4714-a1ef529a964eeb9c21b7521427a91cabb241cc4c3846f67c4b30bf74fe7f1ef13
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
ObjectType-Review-3
content type line 23
ORCID 0000-0003-4888-6771
0000-0002-3630-0537
OpenAccessLink https://pubmed.ncbi.nlm.nih.gov/PMC7267292
PMID 32347966
PQID 2440404653
PQPubID 105515
PageCount 6
ParticipantIDs pubmedcentral_primary_oai_pubmedcentral_nih_gov_7267292
proquest_miscellaneous_2396304398
proquest_journals_2440404653
pubmed_primary_32347966
crossref_citationtrail_10_1002_jmv_25954
crossref_primary_10_1002_jmv_25954
wiley_primary_10_1002_jmv_25954_JMV25954
ProviderPackageCode CITATION
AAYXX
PublicationCentury 2000
PublicationDate October 2020
PublicationDateYYYYMMDD 2020-10-01
PublicationDate_xml – month: 10
  year: 2020
  text: October 2020
PublicationDecade 2020
PublicationPlace United States
PublicationPlace_xml – name: United States
– name: London
– name: Hoboken
PublicationTitle Journal of medical virology
PublicationTitleAlternate J Med Virol
PublicationYear 2020
Publisher Wiley Subscription Services, Inc
John Wiley and Sons Inc
Publisher_xml – name: Wiley Subscription Services, Inc
– name: John Wiley and Sons Inc
References 2017; 6
2009; 47
2020; 382
2020; 63
2015; 223
2019; 57
2019; 14
2016; 54
2019; 105
2017; 250
2014; 194
2020; 11
2011; 6
2015; 7
2020; 109
2012; 50
2017; 96
2020; 1
2012; 1
2020; 395
2020
2020; 92
2020; 9
2020; 26
2020; 25
2016; 60
2018; 56
2004; 119
e_1_2_5_27_1
e_1_2_5_28_1
e_1_2_5_25_1
e_1_2_5_26_1
e_1_2_5_23_1
e_1_2_5_24_1
Lu H (e_1_2_5_4_1) 2020; 1
e_1_2_5_21_1
e_1_2_5_22_1
e_1_2_5_43_1
e_1_2_5_29_1
e_1_2_5_42_1
Lee NY (e_1_2_5_44_1) 2020
e_1_2_5_20_1
e_1_2_5_41_1
Li Z (e_1_2_5_38_1) 2020
e_1_2_5_15_1
e_1_2_5_14_1
e_1_2_5_39_1
Li R (e_1_2_5_40_1) 2020
e_1_2_5_17_1
e_1_2_5_36_1
e_1_2_5_9_1
e_1_2_5_16_1
e_1_2_5_37_1
e_1_2_5_8_1
e_1_2_5_11_1
e_1_2_5_34_1
e_1_2_5_7_1
e_1_2_5_10_1
e_1_2_5_35_1
e_1_2_5_6_1
e_1_2_5_13_1
e_1_2_5_32_1
e_1_2_5_5_1
e_1_2_5_12_1
e_1_2_5_33_1
e_1_2_5_3_1
e_1_2_5_2_1
Goff J (e_1_2_5_19_1) 2015; 7
e_1_2_5_18_1
e_1_2_5_30_1
e_1_2_5_31_1
32484942 - J Med Virol. 2020 Nov;92(11):2345
References_xml – volume: 60
  start-page: 111
  issue: 1
  year: 2016
  end-page: 120
  article-title: Lateral flow assays
  publication-title: Essays Biochem
– volume: 14
  issue: 2
  year: 2019
  article-title: Comparison of the new fully automated extraction platform eMAG to the MagNA PURE 96 and the well‐established easyMAG for detection of common human respiratory viruses
  publication-title: PLoS One
– volume: 105
  start-page: 93
  year: 2019
  end-page: 116
  article-title: Structural insights into coronavirus entry
  publication-title: Adv Virus Res
– volume: 63
  start-page: 706
  issue: 5
  year: 2020
  end-page: 711
  article-title: Clinical characteristics of 24 asymptomatic infections with COVID‐19 screened among close contacts in Nanjing, China
  publication-title: Sci China: Life Sci
– volume: 56
  issue: 3
  year: 2018
  article-title: Parallel validation of three molecular devices for simultaneous detection and identification of influenza A and B and respiratory syncytial viruses
  publication-title: J Clin Microbiol
– volume: 6
  start-page: e49
  issue: 6
  year: 2017
  end-page: 7
  article-title: Additional molecular testing of saliva specimens improves the detection of respiratory viruses
  publication-title: Emerg Microbes Infect
– volume: 6
  issue: 6
  year: 2011
  article-title: Comparison of nasopharyngeal and oropharyngeal swabs for the diagnosis of eight respiratory viruses by real‐time reverse transcription‐PCR assays
  publication-title: PLoS One
– volume: 395
  start-page: 1101
  issue: 10230
  year: 2020
  end-page: 1102
  article-title: Developing antibody tests for SARS‐CoV‐2
  publication-title: Lancet
– volume: 25
  issue: 9
  year: 2020
  article-title: Evaluation of a quantitative RT‐PCR assay for the detection of the emerging coronavirus SARS‐CoV‐2 using a high throughput system
  publication-title: Euro Surveill
– volume: 194
  start-page: 175
  year: 2014
  end-page: 183
  article-title: Serological assays for emerging coronaviruses: challenges and pitfalls
  publication-title: Virus Res
– volume: 50
  start-page: 2282
  issue: 7
  year: 2012
  end-page: 2288
  article-title: Comparison of the Luminex xTAG RVP Fast assay and the Idaho Technology FilmArray RP assay for detection of respiratory viruses in pediatric patients at a cancer hospital
  publication-title: J Clin Microbiol
– volume: 119
  start-page: 195
  issue: 2
  year: 2004
  end-page: 198
  article-title: Virus inactivation by nucleic acid extraction reagents
  publication-title: J Virol Methods
– year: 2020
  article-title: Novel coronavirus and old lessons ‐ preparing the health system for the pandemic [published online ahead of print March 25, 2020]
  publication-title: N Engl J Med
– year: 2020
  article-title: Virological assessment of hospitalized patients with COVID‐2019 [published online ahead of print April 1, 2020]
  publication-title: Nature
– volume: 1
  start-page: 107
  issue: 3
  year: 2012
  end-page: 124
  article-title: Integrated amplification microarrays for infectious disease diagnostics
  publication-title: Microarrays (Basel)
– volume: 26
  issue: 7
  year: 2020
  article-title: Severe acute respiratory syndrome coronavirus 2‐specific antibody responses in coronavirus disease 2019 patients
  publication-title: Emerg Infect Dis
– year: 2020
  article-title: A case of COVID‐19 and pneumonia returning from Macau in Taiwan: clinical course and anti‐SARS‐CoV‐2 IgG dynamic [published online ahead of print March 10, 2016]
  publication-title: J Microbiol Immunol Infect
– volume: 223
  start-page: 13
  year: 2015
  end-page: 18
  article-title: Inactivation and safety testing of Middle East respiratory syndrome coronavirus
  publication-title: J Virol Methods
– volume: 109
  year: 2020
  article-title: The epidemiology and pathogenesis of coronavirus disease (COVID‐19) outbreak
  publication-title: J Autoimmun
– volume: 47
  start-page: 3881
  issue: 12
  year: 2009
  end-page: 3886
  article-title: Multicenter comparative evaluation of five commercial methods for toxoplasma DNA extraction from amniotic fluid
  publication-title: J Clin Microbiol
– volume: 395
  start-page: 1015
  issue: 10229
  year: 2020
  end-page: 1018
  article-title: COVID‐19: towards controlling of a pandemic
  publication-title: Lancet
– volume: 1
  start-page: 25738
  issue: 10
  year: 2020
  end-page: 547
  article-title: The Wuhan SARS‐CoV‐2‐what's next for China
  publication-title: J Med Virol
– volume: 57
  issue: 2
  year: 2019
  article-title: Multicenter evaluation of NeuMoDx group B assay on the NeuMoDx 288 molecular system
  publication-title: J Clin Microbiol
– volume: 382
  issue: 15
  year: 2020
  article-title: Audio interview: emerging tools in the fight against Covid‐19
  publication-title: N Engl J Med
– year: 2020
  article-title: Substantial undocumented infection facilitates the rapid dissemination of novel coronavirus (SARS‐CoV2) [published online ahead of print March 16, 2020]
  publication-title: Science
– volume: 9
  start-page: 680
  issue: 1
  year: 2020
  end-page: 686
  article-title: Establishment and validation of a pseudovirus neutralization assay for SARS‐CoV‐2
  publication-title: Emerg Microbes Infect
– year: 2020
  article-title: The laboratory diagnosis of COVID‐19 infection: current issues and challenges [published online ahead of print April 03, 2020]
  publication-title: J Clin Microbiol
– year: 2020
  article-title: Antibody responses to SARS‐CoV‐2 in patients of novel coronavirus disease 2019 [published online ahead of print March 28, 2020]
  publication-title: Clin Infect Dis
– volume: 56
  issue: 11
  year: 2018
  article-title: Comparison of six sample‐to‐answer influenza A/B and respiratory syncytial virus nucleic acid amplification assays using respiratory specimens from children
  publication-title: J Clin Microbiol
– volume: 9
  start-page: 386
  issue: 1
  year: 2020
  end-page: 389
  article-title: Molecular and serological investigation of 2019‐nCoV infected patients: implication of multiple shedding routes
  publication-title: Emerg Microbes Infect
– volume: 11
  issue: 2
  year: 2020
  article-title: Report from the American Society for Microbiology COVID‐19 International Summit, 23 March 2020: value of diagnostic testing for SARS‐CoV‐2/COVID‐19
  publication-title: mBio
– volume: 54
  start-page: 1912
  issue: 7
  year: 2016
  end-page: 1914
  article-title: Clinical evaluation of the Luminex NxTAG respiratory pathogen panel
  publication-title: J Clin Microbiol
– volume: 9
  start-page: 747
  issue: 1
  year: 2020
  end-page: 756
  article-title: Laboratory diagnosis of emerging human coronavirus infections — the state of the art
  publication-title: Emerg Microbes Infect
– year: 2020
  article-title: Evaluation of saline, phosphate buffered saline and minimum essential medium as potential alternatives to viral transport media for SARS‐CoV‐2 testing [published online ahead of print March 30, 2020]
  publication-title: J Clin Microbiol
– volume: 250
  start-page: 34
  year: 2017
  end-page: 40
  article-title: The effect of a non‐denaturing detergent and a guanidinium‐based inactivation agent on the viability of Ebola virus in mock clinical serum samples
  publication-title: J Virol Methods
– issue: 10
  year: 2020
  article-title: Development and clinical application of a rapid IgM‐IgG combined antibody test for SARS‐CoV‐2 infection diagnosis [published online ahead of print February 27, 2020]
  publication-title: J Med Virol
– volume: 92
  start-page: 401
  issue: 4
  year: 2020
  end-page: 402
  article-title: Outbreak of pneumonia of unknown etiology in Wuhan, China: the mystery and the miracle
  publication-title: J Med Virol
– volume: 96
  start-page: 49
  year: 2017
  end-page: 53
  article-title: Multicenter comparison of the new Cobas 6800 system with Cobas Ampliprep/Cobas TaqMan and Abbott RealTime for the quantification of HIV, HBV and HCV viral load
  publication-title: J Clin Virol
– volume: 50
  start-page: 1064
  issue: 3
  year: 2012
  end-page: 1065
  article-title: Evaluation of swabs, transport media, and specimen transport conditions for optimal detection of viruses by PCR
  publication-title: J Clin Microbiol
– year: 2020
  article-title: COVID‐19 and the risk to health care workers: a case report [published online ahead of print March 16, 2020]
  publication-title: Ann Intern Med
– volume: 7
  year: 2015
  article-title: Surveillance of acute respiratory infections using community‐submitted symptoms and specimens for molecular diagnostic testing
  publication-title: PLoS Curr
– year: 2020
  article-title: Consistent detection of 2019 novel coronavirus in saliva [published online ahead of print February 12, 2020]
  publication-title: Clin Infect Dis
– year: 2020
  article-title: Performance of VivaDiagTM COVID‐19 IgM/IgG rapid test is inadequate for diagnosis of COVID‐19 in acute patients referring to emergency room department [published online ahead of print March 30, 2020]
  publication-title: J Med Virol
– ident: e_1_2_5_20_1
  doi: 10.1371/journal.pone.0021610
– ident: e_1_2_5_9_1
  doi: 10.1128/JCM.06186-11
– ident: e_1_2_5_25_1
  doi: 10.1093/cid/ciaa149
– ident: e_1_2_5_10_1
  doi: 10.1128/JCM.01691-17
– ident: e_1_2_5_16_1
  doi: 10.1128/JCM.01324-18
– ident: e_1_2_5_30_1
  doi: 10.1016/j.jviromet.2017.09.020
– start-page: eabb3221
  year: 2020
  ident: e_1_2_5_40_1
  article-title: Substantial undocumented infection facilitates the rapid dissemination of novel coronavirus (SARS‐CoV2) [published online ahead of print March 16, 2020]
  publication-title: Science
– ident: e_1_2_5_6_1
  doi: 10.1016/j.jaut.2020.102433
– ident: e_1_2_5_34_1
  doi: 10.1016/j.virusres.2014.03.018
– ident: e_1_2_5_8_1
  doi: 10.3390/microarrays1030107
– ident: e_1_2_5_11_1
  doi: 10.1080/22221751.2020.1743767
– ident: e_1_2_5_3_1
  doi: 10.1056/NEJMp2005118
– ident: e_1_2_5_17_1
  doi: 10.1128/JCM.00930-18
– ident: e_1_2_5_22_1
  doi: 10.1128/JCM.00590-20
– ident: e_1_2_5_23_1
  doi: 10.1080/22221751.2020.1745095
– issue: 10
  year: 2020
  ident: e_1_2_5_38_1
  article-title: Development and clinical application of a rapid IgM‐IgG combined antibody test for SARS‐CoV‐2 infection diagnosis [published online ahead of print February 27, 2020]
  publication-title: J Med Virol
– ident: e_1_2_5_24_1
  doi: 10.1128/JCM.00512-20
– ident: e_1_2_5_26_1
  doi: 10.1371/journal.pone.0211079
– ident: e_1_2_5_28_1
  doi: 10.1128/JCM.01164-09
– ident: e_1_2_5_32_1
  doi: 10.1016/S0140-6736(20)30788-1
– ident: e_1_2_5_37_1
  doi: 10.1042/EBC20150012
– ident: e_1_2_5_2_1
  doi: 10.1016/S0140-6736(20)30673-5
– ident: e_1_2_5_18_1
  doi: 10.1128/JCM.06551-11
– volume: 7
  year: 2015
  ident: e_1_2_5_19_1
  article-title: Surveillance of acute respiratory infections using community‐submitted symptoms and specimens for molecular diagnostic testing
  publication-title: PLoS Curr
– ident: e_1_2_5_35_1
  doi: 10.1016/bs.aivir.2019.08.002
– ident: e_1_2_5_15_1
  doi: 10.1016/j.jcv.2017.09.007
– ident: e_1_2_5_31_1
  doi: 10.1016/j.jviromet.2015.07.002
– ident: e_1_2_5_7_1
  doi: 10.1056/NEJMe2009651
– ident: e_1_2_5_5_1
  doi: 10.1002/jmv.25678
– ident: e_1_2_5_12_1
  doi: 10.7326/L20-0175
– ident: e_1_2_5_36_1
  doi: 10.1080/22221751.2020.1729071
– ident: e_1_2_5_14_1
  doi: 10.1128/JCM.00482-16
– ident: e_1_2_5_39_1
  doi: 10.1007/s11427-020-1661-4
– ident: e_1_2_5_42_1
  doi: 10.1128/mBio.00722-20
– ident: e_1_2_5_29_1
  doi: 10.1016/j.jviromet.2004.03.015
– ident: e_1_2_5_27_1
  doi: 10.1002/jmv.25800
– ident: e_1_2_5_43_1
  doi: 10.1038/s41586-020-2196-x
– ident: e_1_2_5_41_1
  doi: 10.1093/cid/ciaa344
– volume: 1
  start-page: 25738
  issue: 10
  year: 2020
  ident: e_1_2_5_4_1
  article-title: The Wuhan SARS‐CoV‐2‐what's next for China
  publication-title: J Med Virol
– ident: e_1_2_5_33_1
  doi: 10.3201/eid2607.200841
– start-page: S1684
  year: 2020
  ident: e_1_2_5_44_1
  article-title: A case of COVID‐19 and pneumonia returning from Macau in Taiwan: clinical course and anti‐SARS‐CoV‐2 IgG dynamic [published online ahead of print March 10, 2016]
  publication-title: J Microbiol Immunol Infect
– ident: e_1_2_5_13_1
  doi: 10.2807/1560-7917.ES.2020.25.9.2000152
– ident: e_1_2_5_21_1
  doi: 10.1038/emi.2017.35
– reference: 32484942 - J Med Virol. 2020 Nov;92(11):2345
SSID ssj0008922
Score 2.4121413
SecondaryResourceType review_article
Snippet As the 2019 novel coronavirus disease (COVID‐19) outbreak has evolved in each country, the approach to the laboratory assessment of severe acute respiratory...
As the 2019 novel coronavirus disease (COVID-19) outbreak has evolved in each country, the approach to the laboratory assessment of severe acute respiratory...
SourceID pubmedcentral
proquest
pubmed
crossref
wiley
SourceType Open Access Repository
Aggregation Database
Index Database
Enrichment Source
Publisher
StartPage 1812
SubjectTerms Algorithms
Antibodies, Viral - immunology
assessment
China
Coronaviridae
Coronaviruses
COVID-19
COVID-19 - diagnosis
COVID-19 - immunology
COVID-19 - virology
Evolution
Humans
Immunoassay
Laboratories
molecular testing
Outbreaks
Pandemics - prevention & control
Pneumonia, Viral - diagnosis
Pneumonia, Viral - immunology
Pneumonia, Viral - virology
Review
Reviews
SARS-CoV-2 - immunology
Serologic Tests - methods
Serology
Severe acute respiratory syndrome
Severe acute respiratory syndrome coronavirus 2
Viral diseases
Virology
Title An evolving approach to the laboratory assessment of COVID‐19
URI https://onlinelibrary.wiley.com/doi/abs/10.1002%2Fjmv.25954
https://www.ncbi.nlm.nih.gov/pubmed/32347966
https://www.proquest.com/docview/2440404653
https://www.proquest.com/docview/2396304398
https://pubmed.ncbi.nlm.nih.gov/PMC7267292
Volume 92
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnZ3NTtwwEIBHiAPqhdIW2qVQuRWHXrIkjhOvxQEhWkSRFqSqIA5Ike211fKTVLBbCU59hD4jT8KM80O3UKnqLZLHiuPxeCYe-zPAWiqkHFjjojTXg0hY4SJDSV5uhdE6H0kViDfD_Xz3UOwdZ8czsNGehan5EN2CG1lGmK_JwLW5Wr-Hhp5e_Ohj7J4RC5T2alFA9PkeHTVQdQYBZ4IIfVDWUoVivt7VnPZFDwLMh_skf49fgwPaeQonbdPrfSdn_cnY9O3NH1TH__y2BZhvAlO2VY-kZzDjyucwN2xS7y9gc6tkDucyWoBgLYmcjSuGESRrhlJ1ec10h_pklWfbB0efPtz-_JWoRTjc-fhlezdqbl-ILDosEenE-YwrrXLhnFGWJ0ZmBGiTWiVWG4O-31phU4xgfC5Rt2lsvBTeSY9Vk3QJZsuqdK-AKZ-IhI-EI75g7GKjUsO9ECMZKzfygx68b_VQ2AZNTjdknBc1VJkX2CFF6JAevOtEv9c8jseEVlplFo1JXhWcSIgx4eR68LYrRmOiDIkuXTVBmRTnIzosjE16Weu-e0vK6dBtnvdATo2KToBA3dMl5bevAdgteY7_MBw_Myj97w0v9oZH4WH530VfwxNOawBhg-EKzI4vJ24VA6WxeRMs4g5yYA-1
linkProvider Wiley-Blackwell
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnZ1Lb9QwEIBHpZWAC1CeWwoYxIFLtonjxGupEqr60LZ0i4TaqhcU2V5bLY8Eld1KcOIn8Bv7S5hxHmUplRC3SB4rjsfjmYztzwAvUyHlwBoXpbkeRMIKFxla5OVWGK3zsVSBeDPay4cHYucoO5qD1fYsTM2H6BJuZBlhviYDp4T0ygU19MPnsz4G75m4Bgt0ozeR8zfeXcCjBqpeQ8C5IEIvlLVcoZivdFVnvdGlEPPyTsnfI9jggrZuw_u28fXOk4_96cT07fc_uI7_-3V34FYTm7K1ejAtwpwr78L1UbP6fg9er5XM4XRGOQjWwsjZpGIYRLJmNFWn35juaJ-s8mz97eH2xvmPn4m6Dwdbm_vrw6i5gCGy6LNEpBPnM660yoVzRlmeGJkRo01qlVhtDLp_a4VNMYjxuUT1prHxUngnPVZN0gcwX1alewRM-UQkfCwcIQZjFxuVGu6FGMtYubEf9OBVq4jCNnRyuiTjU1FzlXmBHVKEDunBi070S43k-JvQcqvNorHKrwUnGGJMRLkePO-K0Z5okUSXrpqiTIpTEp0XxiY9rJXfvSXldO42z3sgZ4ZFJ0Cs7tmS8uQ4MLslz_E3huNnBq1f3fBiZ3QYHpb-XfQZ3Bjuj3aL3e29N4_hJqeUQNhvuAzzk9Ope4Jx08Q8DebxC1jFE9E
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnZ1bTxQxFIBPEBPiC-KVFZRqfPBllplOp93GB0NYNoAuGiOEB5NJ22njBWYI7JroEz_B38gv4bRzwRVNjG-T9DTT6em5TC9fAZ6nTIiB0TZKuRpEzDAbab_ISw3TSvFCyEC8Ge_x7X22e5gdzsHL9ixMzYfoJty8ZQR_7Q38pHDrV9DQL8ff-pi7Z-wG3GQ8lv7ehuH7K3bUQNZLCOgKIgxCWYsViul6V3U2GF3LMK9vlPw1gQ0RaHQbPrZtrzeefO1PJ7pvfvyGdfzPj1uCxSYzJRv1ULoDc7a8CwvjZu39HrzaKIlFZ-ZnIEiLIieTimAKSZqxVJ1-J6pjfZLKkc23BzvDi_OfibwP-6OtD5vbUXP9QmQwYrFIJdZlVCrJmbVaGppokXlCm1AyMUprDP7GMJNiCuO4QOWmsXaCOSscVk3SBzBfVqVdBiJdwhJaMOsBg7GNtUw1dYwVIpa2cIMevGj1kJuGTe6vyDjKa6oyzbFD8tAhPXjWiZ7UQI4_Ca22yswbmzzLqUchxp4n14OnXTFak18iUaWtpiiTokPyp4WxSQ9r3XdvSak_dct5D8TMqOgEPKl7tqT8_CkQuwXl-BND8TOD0v_e8Hx3fBAeHv276BosvBuO8jc7e69X4Bb18wFhs-EqzE9Op_YxJk0T_SQYxyUXURKA
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=An+evolving+approach+to+the+laboratory+assessment+of+COVID-19&rft.jtitle=Journal+of+medical+virology&rft.au=Lu%2C+Hongzhou&rft.au=Stratton%2C+Charles+W&rft.au=Tang%2C+Yi-Wei&rft.date=2020-10-01&rft.issn=1096-9071&rft.eissn=1096-9071&rft.volume=92&rft.issue=10&rft.spage=1812&rft_id=info:doi/10.1002%2Fjmv.25954&rft.externalDBID=NO_FULL_TEXT
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0146-6615&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0146-6615&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0146-6615&client=summon