Sensitive fluorescent hybridisation protocol development for simultaneous detection of microRNA and cellular marker proteins (in the retina)

Nowadays, increasing number of microRNAs are found to have crucial roles in various physiological processes through gene expression regulation via RNA silencing as a result of base pairing with complementary mRNA sequences. To reveal the spatial distribution of microRNA expression in tissues, in sit...

Full description

Saved in:
Bibliographic Details
Published inHistochemistry and cell biology Vol. 150; no. 5; pp. 557 - 566
Main Authors Kovács-Valasek, Andrea, Szalontai, Bálint, Sétáló, György, Gábriel, Robert
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer Berlin Heidelberg 01.11.2018
Springer Nature B.V
Subjects
Online AccessGet full text

Cover

Loading…
Abstract Nowadays, increasing number of microRNAs are found to have crucial roles in various physiological processes through gene expression regulation via RNA silencing as a result of base pairing with complementary mRNA sequences. To reveal the spatial distribution of microRNA expression in tissues, in situ hybridisation is the only method developed to date. This work aims to provide a novel approach to obtain information on the possible involvement of microRNA-s in regulatory processes under experimental conditions by enhancing fluorescent detection of microRNA labelling. Developing Wistar rats were used as a model system to analyse retinal microRNA expression in the first 3 postnatal weeks. Using cryosections, the crucial elements of optimal labels were (1) the concentration and duration of proteinase K treatment, (2) hybridisation temperature of microRNA probes and (3) temperature of stringency washes. Further improvements made possible to combine our in situ hybridisation protocol with double-label immunofluorescence allowing for the simultaneous detection of microRNA-s with high sensitivity and a neuronal cell marker and/or a synaptic marker protein. Thus, the regulatory microRNA-s can be localised in an identified cell type along with its potential target protein. We believe that our protocol can be easily adapted for a variety of tissues of different origins, developmental stages and experimental conditions.
AbstractList Nowadays, increasing number of microRNAs are found to have crucial roles in various physiological processes through gene expression regulation via RNA silencing as a result of base pairing with complementary mRNA sequences. To reveal the spatial distribution of microRNA expression in tissues, in situ hybridisation is the only method developed to date. This work aims to provide a novel approach to obtain information on the possible involvement of microRNA-s in regulatory processes under experimental conditions by enhancing fluorescent detection of microRNA labelling. Developing Wistar rats were used as a model system to analyse retinal microRNA expression in the first 3 postnatal weeks. Using cryosections, the crucial elements of optimal labels were (1) the concentration and duration of proteinase K treatment, (2) hybridisation temperature of microRNA probes and (3) temperature of stringency washes. Further improvements made possible to combine our in situ hybridisation protocol with double-label immunofluorescence allowing for the simultaneous detection of microRNA-s with high sensitivity and a neuronal cell marker and/or a synaptic marker protein. Thus, the regulatory microRNA-s can be localised in an identified cell type along with its potential target protein. We believe that our protocol can be easily adapted for a variety of tissues of different origins, developmental stages and experimental conditions.
Author Kovács-Valasek, Andrea
Gábriel, Robert
Sétáló, György
Szalontai, Bálint
Author_xml – sequence: 1
  givenname: Andrea
  surname: Kovács-Valasek
  fullname: Kovács-Valasek, Andrea
  organization: Department of Experimental Zoology and Neurobiology, University of Pécs
– sequence: 2
  givenname: Bálint
  surname: Szalontai
  fullname: Szalontai, Bálint
  organization: János Szentágothai Research Centre, University of Pécs
– sequence: 3
  givenname: György
  surname: Sétáló
  fullname: Sétáló, György
  organization: Department of Medical Biology, University of Pécs
– sequence: 4
  givenname: Robert
  orcidid: 0000-0001-9323-8795
  surname: Gábriel
  fullname: Gábriel, Robert
  email: gabriel@ttk.pte.hu
  organization: Department of Experimental Zoology and Neurobiology, University of Pécs, János Szentágothai Research Centre, University of Pécs
BackLink https://www.ncbi.nlm.nih.gov/pubmed/30088096$$D View this record in MEDLINE/PubMed
BookMark eNp1kctq3TAQhkVJaU7SPkA3RdBNsnA78kWSN4UQeoPQQi_QnZDlcY5SWzqV5AN5hz505DhNL9CFEGi--TX__EfkwHmHhDxl8IIBiJcRoGaygHyYgKbgD8iG1VVZMNZ-OyAbaGtZ8PxySI5ivAJgTVuWj8hhBSAltHxDfn5GF22ye6TDOPuA0aBLdHvdBdvbqJP1ju6CT974kfa4x9HvpgUZfKDRTvOYtEM_x1xMaG55P9DJmuA_fTij2vXU4DjOow500uE7hls9tC7SE-to2iINmKzTp4_Jw0GPEZ_c3cfk65vXX87fFRcf374_P7soTC0gFUNneNlhJw2yoaxMzXgj-roy0A5900hRM9FqFFXDNOgGOskq0bBBltCWHTfVMXm16u7mbsJ-cRz0qHbB5gGvlddW_V1xdqsu_V5xJkveNlng5E4g-B8zxqQmGxeX6ypUCbLhAgRjGX3-D3rl5-CyvYWqOZd1W2WKrVTeWowBh_thGKgla7VmrXLWasla8dzz7E8X9x2_ws1AuQIxl9wlht9f_1_1Bh8Aufc
CitedBy_id crossref_primary_10_1007_s00418_020_01944_z
crossref_primary_10_3390_ijms22137078
crossref_primary_10_3390_ijms22052577
crossref_primary_10_1038_s41598_019_57194_0
Cites_doi 10.1167/iovs.16-20566
10.1016/S0092-8674(04)00045-5
10.1007/978-1-4939-1459-3_7
10.1007/s00125-014-3197-9
10.1002/dneu.22191
10.1016/j.neuroscience.2017.02.003
10.1007/s12035-013-8544-1
10.1007/978-1-60761-657-3_18
10.1038/nprot.2007.153
10.1186/1471-2164-11-715
10.1242/dev.083642
10.14670/HH-18.1219
10.1371/journal.pone.0118949
10.1016/j.pneurobio.2004.04.002
10.3390/ijms17081232
10.1007/s13311-015-0340-3
10.1038/nprot.2010.62
10.3109/10520295.2013.839825
10.1016/j.ymeth.2007.04.008
10.1016/j.neuint.2013.11.005
10.3389/fnmol.2016.00126
10.1038/nprot.2007.313
10.1186/s12864-015-1971-9
10.1055/s-0034-1397344
10.1002/stem.2093
10.3390/ijms160613259
ContentType Journal Article
Copyright The Author(s) 2018
Histochemistry and Cell Biology is a copyright of Springer, (2018). All Rights Reserved. © 2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Copyright_xml – notice: The Author(s) 2018
– notice: Histochemistry and Cell Biology is a copyright of Springer, (2018). All Rights Reserved. © 2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
DBID C6C
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
3V.
7QP
7RV
7TK
7X7
7XB
88A
88E
8AO
8C1
8FE
8FH
8FI
8FJ
8FK
ABUWG
AFKRA
AZQEC
BBNVY
BENPR
BHPHI
CCPQU
DWQXO
FYUFA
GHDGH
GNUQQ
HCIFZ
K9.
KB0
LK8
M0S
M1P
M7P
NAPCQ
PQEST
PQQKQ
PQUKI
PRINS
7X8
5PM
DOI 10.1007/s00418-018-1705-6
DatabaseName SpringerOpen
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
ProQuest Central (Corporate)
Calcium & Calcified Tissue Abstracts
Nursing & Allied Health Database
Neurosciences Abstracts
Health & Medical Collection
ProQuest Central (purchase pre-March 2016)
Biology Database (Alumni Edition)
Medical Database (Alumni Edition)
ProQuest Pharma Collection
Public Health Database
ProQuest SciTech Collection
ProQuest Natural Science Collection
Hospital Premium Collection
Hospital Premium Collection (Alumni Edition)
ProQuest Central (Alumni) (purchase pre-March 2016)
ProQuest Central (Alumni)
ProQuest Central
ProQuest Central Essentials
Biological Science Collection
ProQuest Central
Natural Science Collection
ProQuest One Community College
ProQuest Central
Health Research Premium Collection
Health Research Premium Collection (Alumni)
ProQuest Central Student
SciTech Premium Collection
ProQuest Health & Medical Complete (Alumni)
Nursing & Allied Health Database (Alumni Edition)
Biological Sciences
Health & Medical Collection (Alumni Edition)
PML(ProQuest Medical Library)
Biological Science Database
Nursing & Allied Health Premium
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
MEDLINE - Academic
PubMed Central (Full Participant titles)
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
ProQuest Central Student
ProQuest Central Essentials
ProQuest Health & Medical Complete (Alumni)
ProQuest Central (Alumni Edition)
SciTech Premium Collection
ProQuest One Community College
ProQuest Natural Science Collection
ProQuest Pharma Collection
ProQuest Central China
ProQuest Biology Journals (Alumni Edition)
ProQuest Central
Health Research Premium Collection
Health and Medicine Complete (Alumni Edition)
Natural Science Collection
ProQuest Central Korea
Biological Science Collection
ProQuest Medical Library (Alumni)
ProQuest Public Health
ProQuest Biological Science Collection
ProQuest One Academic Eastern Edition
ProQuest Nursing & Allied Health Source
ProQuest Hospital Collection
Health Research Premium Collection (Alumni)
Biological Science Database
ProQuest SciTech Collection
Neurosciences Abstracts
ProQuest Hospital Collection (Alumni)
Nursing & Allied Health Premium
ProQuest Health & Medical Complete
ProQuest Medical Library
ProQuest One Academic UKI Edition
ProQuest Nursing & Allied Health Source (Alumni)
ProQuest One Academic
Calcium & Calcified Tissue Abstracts
ProQuest Central (Alumni)
MEDLINE - Academic
DatabaseTitleList MEDLINE
MEDLINE - Academic
ProQuest Central Student


Database_xml – sequence: 1
  dbid: C6C
  name: SpringerOpen
  url: http://www.springeropen.com/
  sourceTypes: Publisher
– sequence: 2
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 3
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
– sequence: 4
  dbid: BENPR
  name: ProQuest Central
  url: https://www.proquest.com/central
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Chemistry
Biology
EISSN 1432-119X
EndPage 566
ExternalDocumentID 10_1007_s00418_018_1705_6
30088096
Genre Journal Article
GrantInformation_xml – fundername: Hungarian Brain Research Program
  grantid: 2017-1.2.1-NKP-2017-00002
– fundername: NKFIH
  grantid: 119289
– fundername: ;
  grantid: 2017-1.2.1-NKP-2017-00002
– fundername: ;
  grantid: 119289
GroupedDBID ---
-4W
-5E
-5G
-BR
-EM
-Y2
-~C
-~X
.55
.86
.GJ
.VR
04C
06C
06D
0R~
0VY
1N0
2.D
203
28-
29I
29~
2J2
2JN
2JY
2KG
2KM
2LR
2P1
2VQ
2~H
30V
36B
3O-
3SX
3V.
4.4
406
408
409
40D
40E
476
53G
5QI
5RE
5VS
67N
67Z
6NX
78A
7RV
7X7
85S
88A
88E
8AO
8C1
8FE
8FH
8FI
8FJ
8TC
8UJ
95-
95.
95~
96X
AAAVM
AABHQ
AABYN
AAFGU
AAHNG
AAIAL
AAJKR
AANXM
AANZL
AAPBV
AARHV
AARTL
AATNV
AATVU
AAUYE
AAWCG
AAYFA
AAYIU
AAYQN
AAYTO
ABBBX
ABBXA
ABDBF
ABDZT
ABECU
ABELW
ABFGW
ABFTV
ABHLI
ABHQN
ABJNI
ABJOX
ABKAS
ABKCH
ABKTR
ABMNI
ABMQK
ABNWP
ABPLI
ABPTK
ABQBU
ABSXP
ABTEG
ABTHY
ABTKH
ABTMW
ABULA
ABUWG
ABWNU
ABXPI
ACBMV
ACBRV
ACBXY
ACBYP
ACGFO
ACGFS
ACHSB
ACHXU
ACIGE
ACIPQ
ACKNC
ACMDZ
ACMLO
ACNCT
ACOKC
ACOMO
ACPRK
ACREN
ACTTH
ACVWB
ACWMK
ADBBV
ADHIR
ADIMF
ADINQ
ADKNI
ADKPE
ADMDM
ADOAH
ADOXG
ADRFC
ADTPH
ADURQ
ADYFF
ADYOE
ADYPR
ADZKW
AEBTG
AEEQQ
AEFIE
AEFTE
AEGAL
AEGNC
AEJHL
AEJRE
AEKMD
AENEX
AEOHA
AEPYU
AEQTP
AESKC
AESTI
AETLH
AEVLU
AEVTX
AEXYK
AFDYV
AFEXP
AFGCZ
AFKRA
AFLOW
AFNRJ
AFQWF
AFWTZ
AFYQB
AFZKB
AGAYW
AGDGC
AGGBP
AGGDS
AGJBK
AGMZJ
AGQMX
AGWIL
AGWZB
AGYKE
AHAVH
AHBYD
AHKAY
AHMBA
AHSBF
AHYZX
AIAKS
AIIXL
AILAN
AIMYW
AITGF
AJBLW
AJDOV
AJRNO
AJZVZ
AKMHD
AKQUC
ALMA_UNASSIGNED_HOLDINGS
ALWAN
AMKLP
AMTXH
AMXSW
AMYLF
AOCGG
AOSHJ
ARMRJ
AXYYD
AZFZN
B-.
B0M
BA0
BBNVY
BBWZM
BDATZ
BENPR
BGNMA
BHPHI
BKEYQ
BMSDO
BPHCQ
BVXVI
C6C
CAG
CCPQU
COF
CSCUP
DDRTE
DL5
DNIVK
DPUIP
DU5
EAD
EAP
EBD
EBLON
EBS
EIHBH
EIOEI
EJD
EMB
EMK
EMOBN
EN4
EPAXT
EPL
ESBYG
ESX
EX3
F5P
FEDTE
FERAY
FFXSO
FIGPU
FINBP
FNLPD
FRRFC
FSGXE
FWDCC
FYUFA
G-Y
G-Z
GGCAI
GGRSB
GJIRD
GNWQR
GQ6
GQ7
GQ8
GXS
HCIFZ
HF~
HG5
HG6
HMCUK
HMJXF
HQYDN
HRMNR
HVGLF
HZ~
I09
IHE
IJ-
IKXTQ
ITM
IWAJR
IXC
IZIGR
IZQ
I~X
I~Z
J-C
J0Z
JBSCW
JCJTX
JZLTJ
KDC
KOV
KOW
KPH
LAS
LK8
LLZTM
M0L
M1P
M4Y
M7P
MA-
N2Q
N9A
NAPCQ
NB0
NDZJH
NPVJJ
NQJWS
NU0
O9-
O93
O9G
O9I
O9J
OAM
P19
P2P
PF0
PQQKQ
PROAC
PSQYO
PT4
PT5
Q2X
QOK
QOR
QOS
R89
R9I
RHV
RIG
RNI
RNS
ROL
RPX
RRX
RSV
RZK
S16
S1Z
S26
S27
S28
S3A
S3B
SAP
SBY
SCLPG
SDH
SDM
SHX
SISQX
SJYHP
SMD
SNE
SNPRN
SNX
SOHCF
SOJ
SPISZ
SRMVM
SSLCW
SSXJD
STPWE
SV3
SZN
T13
T16
TSG
TSK
TSV
TUC
TUS
U2A
U9L
UG4
UKHRP
UNUBA
UOJIU
UTJUX
UZXMN
VC2
VFIZW
W23
W48
WJK
WK6
WK8
WOW
X7M
YLTOR
Z45
Z7U
Z7W
Z82
Z87
Z8O
Z8Q
Z8V
Z91
ZMTXR
ZOVNA
ZXP
~8M
~EX
~KM
AACDK
AAEOY
AAJBT
AAQLM
AASML
AAYZH
ABAKF
ACAOD
ACDTI
ACZOJ
AEFQL
AEMSY
AFBBN
AGQEE
AGRTI
AIGIU
ALIPV
CGR
CUY
CVF
ECM
EIF
H13
NPM
AAYXX
CITATION
7QP
7TK
7XB
8FK
AZQEC
DWQXO
GNUQQ
K9.
PQEST
PQUKI
PRINS
7X8
5PM
ID FETCH-LOGICAL-c470t-fbc62beb8ce1f23c41657d43c09fd55874179ae7351a0a50b813751f82092b6c3
IEDL.DBID BENPR
ISSN 0948-6143
IngestDate Tue Sep 17 21:25:58 EDT 2024
Fri Oct 25 08:18:36 EDT 2024
Thu Oct 10 22:01:46 EDT 2024
Thu Sep 12 18:02:57 EDT 2024
Wed Oct 16 00:59:45 EDT 2024
Sat Dec 16 12:06:31 EST 2023
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 5
Keywords MicroRNA
mir-9
Tyramide signal amplification
mir-23
In situ hybridisation
Immunocytochemistry
Language English
License Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c470t-fbc62beb8ce1f23c41657d43c09fd55874179ae7351a0a50b813751f82092b6c3
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ORCID 0000-0001-9323-8795
OpenAccessLink http://link.springer.com/10.1007/s00418-018-1705-6
PMID 30088096
PQID 2084668493
PQPubID 48619
PageCount 10
ParticipantIDs pubmedcentral_primary_oai_pubmedcentral_nih_gov_6182695
proquest_miscellaneous_2085670711
proquest_journals_2084668493
crossref_primary_10_1007_s00418_018_1705_6
pubmed_primary_30088096
springer_journals_10_1007_s00418_018_1705_6
PublicationCentury 2000
PublicationDate 2018-11-01
PublicationDateYYYYMMDD 2018-11-01
PublicationDate_xml – month: 11
  year: 2018
  text: 2018-11-01
  day: 01
PublicationDecade 2010
PublicationPlace Berlin/Heidelberg
PublicationPlace_xml – name: Berlin/Heidelberg
– name: Germany
– name: New York
PublicationTitle Histochemistry and cell biology
PublicationTitleAbbrev Histochem Cell Biol
PublicationTitleAlternate Histochem Cell Biol
PublicationYear 2018
Publisher Springer Berlin Heidelberg
Springer Nature B.V
Publisher_xml – name: Springer Berlin Heidelberg
– name: Springer Nature B.V
References Obernosterer, Martinez, Alenius (CR17) 2007; 2
Singh, Keirstead, Wolujczyk (CR21) 2014; 89
Bagnoli, Dal Monte, Casini (CR1) 2003; 18
Kovács-Valasek, Szabadfi, Dénes (CR12) 2017; 348
Smit-McBride, Forward, Nguyen (CR22) 2014; 20
Barmada (CR2) 2015; 12
Barter, Tselepi, Gõmez (CR4) 2015; 33
Ryan, Oliveira-Fernandes, Lavker (CR19) 2006; 12
Lakk, Denes, Kovacs (CR13) 2017; 58
Kasai, Kakihara, Miura (CR11) 2016; 9
Song, Ro, Yan (CR23) 2010; 629
Bartel (CR3) 2004; 116
Yang (CR27) 2004; 73
Szabadfi, Szabo, Kiss (CR24) 2014; 64
Chung, Gillies, Sugiyama (CR6) 2015; 10
Mohr, Mott (CR14) 2015; 35
Thompson, Deo, Turner (CR25) 2007; 43
Centanin, Wittbrodt (CR5) 2014; 141
Diaz, Zamboni, Tice, Farci (CR8) 2015
Karali, Peluso, Gennarino (CR10) 2010; 11
Mortuza, Feng, Chakrabarti (CR15) 2014; 57
Nielsen, Møller, Holmstrøm (CR16) 2014; 1211
de Planell-Saguer, Rodicio, Mourelatos (CR7) 2010; 5
Kalani, Tyagi, Tyagi (CR9) 2014; 49
Silahtaroglu, Nolting, Dyrskjøt (CR20) 2007; 2
Urbanek, Nawrocka, Krzyzosiak (CR26) 2015; 16
Rasheed, Sreekanth, Dhanesh (CR18) 2014; 74
Zhou, Hara, Dai (CR28) 2016; 17
15201037 - Prog Neurobiol. 2004 Jun;73(2):127-50
27920667 - Front Mol Neurosci. 2016 Nov 22;9:126
25489229 - Mol Vis. 2014 Nov 05;20:1569-78
24381194 - Development. 2014 Jan;141(2):241-4
25741709 - PLoS One. 2015 Mar 05;10(3):e0118949
12973690 - Histol Histopathol. 2003 Oct;18(4):1219-42
17102797 - Mol Vis. 2006 Oct 17;12:1175-84
17947994 - Nat Protoc. 2007;2(10):2520-8
25218378 - Methods Mol Biol. 2014;1211:77-84
24262293 - Neurochem Int. 2014 Jan;64:84-91
26068454 - Int J Mol Sci. 2015 Jun 10;16(6):13259-86
17889803 - Methods. 2007 Oct;43(2):153-61
20387156 - Methods Mol Biol. 2010;629:287-94
23999871 - Mol Neurobiol. 2014 Feb;49(1):590-600
24106971 - Biotech Histochem. 2014 May;89(4):259-66
26459852 - BMC Genomics. 2015 Oct 12;16:767
24838392 - Dev Neurobiol. 2014 Dec;74(12):1155-71
28125843 - Invest Ophthalmol Vis Sci. 2017 Jan 1;58(1):565-572
24570140 - Diabetologia. 2014 May;57(5):1037-46
21171988 - BMC Genomics. 2010 Dec 20;11:715
26175215 - Stem Cells. 2015 Nov;33(11):3266-80
25689976 - Neurotherapeutics. 2015 Apr;12(2):340-51
27490531 - Int J Mol Sci. 2016 Aug 01;17(8)
20539282 - Nat Protoc. 2010 Jun;5(6):1061-73
25632930 - Semin Liver Dis. 2015 Feb;35(1):3-11
17571058 - Nat Protoc. 2007;2(6):1508-14
14744438 - Cell. 2004 Jan 23;116(2):281-97
28215987 - Neuroscience. 2017 Apr 21;348:1-10
L Centanin (1705_CR5) 2014; 141
P Bagnoli (1705_CR1) 2003; 18
X-L Yang (1705_CR27) 2004; 73
MO Urbanek (1705_CR26) 2015; 16
R Mortuza (1705_CR15) 2014; 57
U Singh (1705_CR21) 2014; 89
BS Nielsen (1705_CR16) 2014; 1211
M Karali (1705_CR10) 2010; 11
AM Mohr (1705_CR14) 2015; 35
A Kalani (1705_CR9) 2014; 49
K Szabadfi (1705_CR24) 2014; 64
M Zhou (1705_CR28) 2016; 17
SJ Barmada (1705_CR2) 2015; 12
G Obernosterer (1705_CR17) 2007; 2
G Diaz (1705_CR8) 2015
AN Silahtaroglu (1705_CR20) 2007; 2
M Lakk (1705_CR13) 2017; 58
RC Thompson (1705_CR25) 2007; 43
Z Smit-McBride (1705_CR22) 2014; 20
SH Chung (1705_CR6) 2015; 10
MJ Barter (1705_CR4) 2015; 33
A Kovács-Valasek (1705_CR12) 2017; 348
DG Ryan (1705_CR19) 2006; 12
A Kasai (1705_CR11) 2016; 9
VA Rasheed (1705_CR18) 2014; 74
DP Bartel (1705_CR3) 2004; 116
R Song (1705_CR23) 2010; 629
M Planell-Saguer de (1705_CR7) 2010; 5
References_xml – volume: 58
  start-page: 565
  year: 2017
  end-page: 572
  ident: CR13
  article-title: Pituitary adenylate cyclase-activating peptide (PACAP), a novel secretagogue, regulates secreted morphogens in newborn rat retina
  publication-title: Investig Ophthalmol Vis Sci
  doi: 10.1167/iovs.16-20566
  contributor:
    fullname: Kovacs
– volume: 20
  start-page: 1569
  year: 2014
  end-page: 1578
  ident: CR22
  article-title: Age-dependent increase in miRNA-34a expression in the posterior pole of the mouse eye
  publication-title: Mol Vis
  contributor:
    fullname: Nguyen
– volume: 116
  start-page: 281
  year: 2004
  end-page: 297
  ident: CR3
  article-title: MicroRNAs
  publication-title: Cell
  doi: 10.1016/S0092-8674(04)00045-5
  contributor:
    fullname: Bartel
– volume: 1211
  start-page: 77
  year: 2014
  end-page: 84
  ident: CR16
  article-title: Chromogen detection of microRNA in frozen clinical tissue samples using LNATM probe technology
  publication-title: Methods Mol Biol
  doi: 10.1007/978-1-4939-1459-3_7
  contributor:
    fullname: Holmstrøm
– volume: 12
  start-page: 1175
  year: 2006
  end-page: 1184
  ident: CR19
  article-title: MicroRNAs of the mammalian eye display distinct and overlapping tissue specificity
  publication-title: Mol Vis
  contributor:
    fullname: Lavker
– volume: 57
  start-page: 1037
  year: 2014
  end-page: 1046
  ident: CR15
  article-title: MiR-195 regulates SIRT1-mediated changes in diabetic retinopathy
  publication-title: Diabetologia
  doi: 10.1007/s00125-014-3197-9
  contributor:
    fullname: Chakrabarti
– volume: 74
  start-page: 1155
  year: 2014
  end-page: 1171
  ident: CR18
  article-title: Developmental wave of Brn3b expression leading to RGC fate specification is synergistically maintained by miR-23a and miR-374
  publication-title: Dev Neurobiol
  doi: 10.1002/dneu.22191
  contributor:
    fullname: Dhanesh
– volume: 348
  start-page: 1
  year: 2017
  end-page: 10
  ident: CR12
  article-title: Accelerated retinal aging in PACAP knock-out mice
  publication-title: Neuroscience
  doi: 10.1016/j.neuroscience.2017.02.003
  contributor:
    fullname: Dénes
– volume: 49
  start-page: 590
  year: 2014
  end-page: 600
  ident: CR9
  article-title: Exosomes: mediators of neurodegeneration, neuroprotection and therapeutics
  publication-title: Mol Neurobiol
  doi: 10.1007/s12035-013-8544-1
  contributor:
    fullname: Tyagi
– volume: 629
  start-page: 287
  year: 2010
  end-page: 294
  ident: CR23
  article-title: In situ hybridization detection of microRNAs
  publication-title: Methods Mol Biol
  doi: 10.1007/978-1-60761-657-3_18
  contributor:
    fullname: Yan
– volume: 2
  start-page: 1508
  year: 2007
  end-page: 1514
  ident: CR17
  article-title: Locked nucleic acid-based in situ detection of microRNAs in mouse tissue sections
  publication-title: Nat Protoc
  doi: 10.1038/nprot.2007.153
  contributor:
    fullname: Alenius
– volume: 11
  start-page: 715
  year: 2010
  ident: CR10
  article-title: miRNeye: a microRNA expression atlas of the mouse eye
  publication-title: BMC Genom
  doi: 10.1186/1471-2164-11-715
  contributor:
    fullname: Gennarino
– volume: 141
  start-page: 241
  year: 2014
  end-page: 244
  ident: CR5
  article-title: Retinal neurogenesis
  publication-title: Development
  doi: 10.1242/dev.083642
  contributor:
    fullname: Wittbrodt
– volume: 18
  start-page: 1219
  year: 2003
  end-page: 1242
  ident: CR1
  article-title: Expression of neuropeptides and their receptors in the developing retina of mammals
  publication-title: Histol Histopathol
  doi: 10.14670/HH-18.1219
  contributor:
    fullname: Casini
– volume: 10
  start-page: e0118949
  year: 2015
  ident: CR6
  article-title: Profiling of microRNAs involved in retinal degeneration caused by selective Müller cell ablation
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0118949
  contributor:
    fullname: Sugiyama
– volume: 73
  start-page: 127
  issue: 2
  year: 2004
  end-page: 150
  ident: CR27
  article-title: Characterization of receptors for glutamate and GABA in retinal neurons
  publication-title: Prog Neurobiol
  doi: 10.1016/j.pneurobio.2004.04.002
  contributor:
    fullname: Yang
– volume: 17
  start-page: 8
  year: 2016
  ident: CR28
  article-title: Circulating organ-specific microRNAs serve as biomarkers in organ-specific diseases: implications for organ allo- and xeno-transplantation
  publication-title: Int J Mol Sci
  doi: 10.3390/ijms17081232
  contributor:
    fullname: Dai
– volume: 12
  start-page: 340
  year: 2015
  end-page: 351
  ident: CR2
  article-title: Linking RNA dysfunction and neurodegeneration in amyotrophic lateral sclerosis
  publication-title: Neurotherapeutics
  doi: 10.1007/s13311-015-0340-3
  contributor:
    fullname: Barmada
– volume: 5
  start-page: 1061
  year: 2010
  end-page: 1073
  ident: CR7
  article-title: Rapid in situ codetection of noncoding RNAs and proteins in cells and formalin-fixed paraffin-embedded tissue sections without protease treatment
  publication-title: Nat Protoc
  doi: 10.1038/nprot.2010.62
  contributor:
    fullname: Mourelatos
– volume: 89
  start-page: 259
  year: 2014
  end-page: 266
  ident: CR21
  article-title: General principles and methods for routine automated microRNA in situ hybridization and double labeling with immunohistochemistry
  publication-title: Biotech Histochem
  doi: 10.3109/10520295.2013.839825
  contributor:
    fullname: Wolujczyk
– volume: 43
  start-page: 153
  year: 2007
  end-page: 161
  ident: CR25
  article-title: Analysis of microRNA expression by in situ hybridization with RNA oligonucleotide probes
  publication-title: Methods
  doi: 10.1016/j.ymeth.2007.04.008
  contributor:
    fullname: Turner
– volume: 64
  start-page: 84
  year: 2014
  end-page: 91
  ident: CR24
  article-title: PACAP promotes neuron survival in early experimental diabetic retinopathy
  publication-title: Neurochem Int
  doi: 10.1016/j.neuint.2013.11.005
  contributor:
    fullname: Kiss
– volume: 9
  start-page: 126
  year: 2016
  ident: CR11
  article-title: Double in situ hybridization for microRNAs and mRNAs in brain tissues
  publication-title: Front Mol Neurosci
  doi: 10.3389/fnmol.2016.00126
  contributor:
    fullname: Miura
– volume: 2
  start-page: 2520
  year: 2007
  end-page: 2528
  ident: CR20
  article-title: Detection of microRNAs in frozen tissue sections by fluorescence in situ hybridization using locked nucleic acid probes and tyramide signal amplification
  publication-title: Nat Protoc
  doi: 10.1038/nprot.2007.313
  contributor:
    fullname: Dyrskjøt
– year: 2015
  ident: CR8
  article-title: Integrated ordination of miRNA and mRNA expression profiles
  publication-title: BMC Genom
  doi: 10.1186/s12864-015-1971-9
  contributor:
    fullname: Farci
– volume: 35
  start-page: 3
  year: 2015
  end-page: 11
  ident: CR14
  article-title: Overview of microRNA biology
  publication-title: Semin Liver Dis
  doi: 10.1055/s-0034-1397344
  contributor:
    fullname: Mott
– volume: 33
  start-page: 3266
  year: 2015
  end-page: 3280
  ident: CR4
  article-title: Genome-wide microRNA and gene analysis of mesenchymal stem cell chondrogenesis identifies an essential role and multiple targets for miR-140-5p
  publication-title: Stem Cells
  doi: 10.1002/stem.2093
  contributor:
    fullname: Gõmez
– volume: 16
  start-page: 13259
  year: 2015
  end-page: 13286
  ident: CR26
  article-title: Small RNA detection by in situ hybridization methods
  publication-title: Int J Mol Sci
  doi: 10.3390/ijms160613259
  contributor:
    fullname: Krzyzosiak
– year: 2015
  ident: 1705_CR8
  publication-title: BMC Genom
  doi: 10.1186/s12864-015-1971-9
  contributor:
    fullname: G Diaz
– volume: 49
  start-page: 590
  year: 2014
  ident: 1705_CR9
  publication-title: Mol Neurobiol
  doi: 10.1007/s12035-013-8544-1
  contributor:
    fullname: A Kalani
– volume: 35
  start-page: 3
  year: 2015
  ident: 1705_CR14
  publication-title: Semin Liver Dis
  doi: 10.1055/s-0034-1397344
  contributor:
    fullname: AM Mohr
– volume: 73
  start-page: 127
  issue: 2
  year: 2004
  ident: 1705_CR27
  publication-title: Prog Neurobiol
  doi: 10.1016/j.pneurobio.2004.04.002
  contributor:
    fullname: X-L Yang
– volume: 12
  start-page: 1175
  year: 2006
  ident: 1705_CR19
  publication-title: Mol Vis
  contributor:
    fullname: DG Ryan
– volume: 20
  start-page: 1569
  year: 2014
  ident: 1705_CR22
  publication-title: Mol Vis
  contributor:
    fullname: Z Smit-McBride
– volume: 5
  start-page: 1061
  year: 2010
  ident: 1705_CR7
  publication-title: Nat Protoc
  doi: 10.1038/nprot.2010.62
  contributor:
    fullname: M Planell-Saguer de
– volume: 58
  start-page: 565
  year: 2017
  ident: 1705_CR13
  publication-title: Investig Ophthalmol Vis Sci
  doi: 10.1167/iovs.16-20566
  contributor:
    fullname: M Lakk
– volume: 2
  start-page: 1508
  year: 2007
  ident: 1705_CR17
  publication-title: Nat Protoc
  doi: 10.1038/nprot.2007.153
  contributor:
    fullname: G Obernosterer
– volume: 9
  start-page: 126
  year: 2016
  ident: 1705_CR11
  publication-title: Front Mol Neurosci
  doi: 10.3389/fnmol.2016.00126
  contributor:
    fullname: A Kasai
– volume: 74
  start-page: 1155
  year: 2014
  ident: 1705_CR18
  publication-title: Dev Neurobiol
  doi: 10.1002/dneu.22191
  contributor:
    fullname: VA Rasheed
– volume: 12
  start-page: 340
  year: 2015
  ident: 1705_CR2
  publication-title: Neurotherapeutics
  doi: 10.1007/s13311-015-0340-3
  contributor:
    fullname: SJ Barmada
– volume: 33
  start-page: 3266
  year: 2015
  ident: 1705_CR4
  publication-title: Stem Cells
  doi: 10.1002/stem.2093
  contributor:
    fullname: MJ Barter
– volume: 89
  start-page: 259
  year: 2014
  ident: 1705_CR21
  publication-title: Biotech Histochem
  doi: 10.3109/10520295.2013.839825
  contributor:
    fullname: U Singh
– volume: 141
  start-page: 241
  year: 2014
  ident: 1705_CR5
  publication-title: Development
  doi: 10.1242/dev.083642
  contributor:
    fullname: L Centanin
– volume: 64
  start-page: 84
  year: 2014
  ident: 1705_CR24
  publication-title: Neurochem Int
  doi: 10.1016/j.neuint.2013.11.005
  contributor:
    fullname: K Szabadfi
– volume: 17
  start-page: 8
  year: 2016
  ident: 1705_CR28
  publication-title: Int J Mol Sci
  doi: 10.3390/ijms17081232
  contributor:
    fullname: M Zhou
– volume: 11
  start-page: 715
  year: 2010
  ident: 1705_CR10
  publication-title: BMC Genom
  doi: 10.1186/1471-2164-11-715
  contributor:
    fullname: M Karali
– volume: 43
  start-page: 153
  year: 2007
  ident: 1705_CR25
  publication-title: Methods
  doi: 10.1016/j.ymeth.2007.04.008
  contributor:
    fullname: RC Thompson
– volume: 16
  start-page: 13259
  year: 2015
  ident: 1705_CR26
  publication-title: Int J Mol Sci
  doi: 10.3390/ijms160613259
  contributor:
    fullname: MO Urbanek
– volume: 116
  start-page: 281
  year: 2004
  ident: 1705_CR3
  publication-title: Cell
  doi: 10.1016/S0092-8674(04)00045-5
  contributor:
    fullname: DP Bartel
– volume: 10
  start-page: e0118949
  year: 2015
  ident: 1705_CR6
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0118949
  contributor:
    fullname: SH Chung
– volume: 348
  start-page: 1
  year: 2017
  ident: 1705_CR12
  publication-title: Neuroscience
  doi: 10.1016/j.neuroscience.2017.02.003
  contributor:
    fullname: A Kovács-Valasek
– volume: 2
  start-page: 2520
  year: 2007
  ident: 1705_CR20
  publication-title: Nat Protoc
  doi: 10.1038/nprot.2007.313
  contributor:
    fullname: AN Silahtaroglu
– volume: 57
  start-page: 1037
  year: 2014
  ident: 1705_CR15
  publication-title: Diabetologia
  doi: 10.1007/s00125-014-3197-9
  contributor:
    fullname: R Mortuza
– volume: 18
  start-page: 1219
  year: 2003
  ident: 1705_CR1
  publication-title: Histol Histopathol
  doi: 10.14670/HH-18.1219
  contributor:
    fullname: P Bagnoli
– volume: 1211
  start-page: 77
  year: 2014
  ident: 1705_CR16
  publication-title: Methods Mol Biol
  doi: 10.1007/978-1-4939-1459-3_7
  contributor:
    fullname: BS Nielsen
– volume: 629
  start-page: 287
  year: 2010
  ident: 1705_CR23
  publication-title: Methods Mol Biol
  doi: 10.1007/978-1-60761-657-3_18
  contributor:
    fullname: R Song
SSID ssj0015922
Score 2.3039649
Snippet Nowadays, increasing number of microRNAs are found to have crucial roles in various physiological processes through gene expression regulation via RNA...
SourceID pubmedcentral
proquest
crossref
pubmed
springer
SourceType Open Access Repository
Aggregation Database
Index Database
Publisher
StartPage 557
SubjectTerms Animals
Biochemistry
Biomarkers - analysis
Biomedical and Life Sciences
Biomedicine
Cell Biology
Developmental Biology
DNA probes
Endopeptidase K
Gene expression
Gene regulation
Immunofluorescence
Immunohistochemistry
In Situ Hybridization, Fluorescence
Labeling
MicroRNAs
MicroRNAs - analysis
MicroRNAs - metabolism
miRNA
Neurons - chemistry
Neurons - cytology
Neurons - metabolism
Original Paper
Proteinase
Proteins - analysis
Proteins - metabolism
Rats
Rats, Wistar
Retina
Retina - chemistry
Retina - cytology
Retina - metabolism
RNA-mediated interference
Spatial distribution
SummonAdditionalLinks – databaseName: SpringerLink Journals (ICM)
  dbid: U2A
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1Lb9QwEB6VIgQXBOUVKMhIHHgoKIlfyXFVUVVI9ACs1FtkO7a6KnXQPg79D_xoZpxNqqVw4GwrTvxN7G88n2cA3iADb4zzIe8sFzkx6NxyL3JTK6GcMaFIeba_nKqTufh8Js_2oJqOLuLFxzEimRbq6a4bZYYi3RU6PbqQuboFt5E7CJJxzavZFDmQzRA5aESNbpHgYyTzb4_Y3YtuEMybOsk_gqVpDzp-APe35JHNBrQfwp6PB3BnKCd5dQB3j8bqbY_g1zdSptNaxsKPTb8csjax8yu6obWV8DBK0tCjJbDuWjrEkMWy1YKEhib6frPCxnXSa0XWB3ZJAr6vpzNmYsfo1J9krOySRD7L9Dy_iCv2dhEZMktGVySjefcY5sefvh-d5NvKC7kTuljnwTpVWW9r58tQcYcYSt0J7oomdFLWmuqWGa-5LE1hZGHrkmtZBqQTTWWV409gP_bRPwOmhFeutF3QSCSE9402pTRSaWGMK0ydwfsRg_bnkGCjnVIpJ8BaBKwlwFqVweGIUrv911ZtVSCHUrVoeAavp2acbpqEYaaoDw6JdKrM4OkA6jQaRxpUoyeXgd6Be-pAGbh3W-LiPGXiVuSdNTKDD6NhXL_WPz_i-X_1fgH3qmSxdOpzCPvr5ca_RB60tq-S4f8G2KADBA
  priority: 102
  providerName: Springer Nature
Title Sensitive fluorescent hybridisation protocol development for simultaneous detection of microRNA and cellular marker proteins (in the retina)
URI https://link.springer.com/article/10.1007/s00418-018-1705-6
https://www.ncbi.nlm.nih.gov/pubmed/30088096
https://www.proquest.com/docview/2084668493
https://search.proquest.com/docview/2085670711
https://pubmed.ncbi.nlm.nih.gov/PMC6182695
Volume 150
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lj9MwEB6xrRBcECyvwFIZiQMPRUriV3JCpeqyAlGhhUrlFDmOo63EJkvTHvY_8KOZyasqK7jkEEdJ7G9if-P5MgPwChl4Yqwr_DzjwicG7WfcCd_ESihrTBE0eba_LNTZUnxayVW34VZ3ssp-Tmwm6ryytEeOTjqulCoWCX9_9cunqlEUXe1KaBzBOEJPIRrB-MN88fV8iCPIpI0jJCJGJ0nwPq4ZtGlEQxJyoRelA-mrw5XpBt28qZr8K3TarEin9-FeRyXZtMX-Adxy5THcbotLXh_DnVlfy-0h_P5GOnWa2Vjxc1dt2hxO7OKa_tfqBD2MUjZUaBcs3wuJGHJaVq9JdmhKV-1qbNw26q2SVQW7JDnf-WLKTJkzigGQqJVdkuRn09zPrcuavV6XDHkmox8mS_PmESxP599nZ35Xh8G3Qgdbv8isijKXxdaFRcQtIip1LrgNkiKXMtZUxcw4zWVoAiODLA65lmGB5CKJMmX5YxiVVemeAlPCKRtmeaGRVgjnEm1CaaTSwhgbmNiDtz0G6VWbbiMdEis3gKUIWEqApcqDkx6ltPvy6nRvJx68HJpxuGkQ2pGia_CRSK5CD560oA5P40iKYvTrPNAHcA8XUD7uw5ZyfdHk5VbkqyXSg3e9Yexf65-dePb_TjyHu1FjorTpcwKj7WbnXiAN2mYTONIrjcd4Fk5gPP344_N80tk_np2pGR6X0fQPlOMNaw
link.rule.ids 230,315,783,787,888,12070,12237,21402,27938,27939,31733,31734,33280,33281,33758,33759,41095,41134,41537,42164,42203,42606,43324,43593,43819,51590,52125,52248,74081,74350,74638
linkProvider ProQuest
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9QwEB5BESoXBOWVUsBIHHgoUrJ-JSdUVVQLtHuAVtpbZDuOuhJ12s3uof-BH81MXqulgrOjzcbfxP7G8-UbgHfIwHPjfBWXlouYGHRsuRexyZRQzpgqaX22T2dqei6-zeW8P3BrelnlsCa2C3VZOzojxyQdd0qViZx_vrqOqWsUVVf7Fhp34R75cJF3vp6PCRfu1F0VIRcZpkiCD1XNpDMRTUnGhTmUTmSstvelW2Tztmbyr8Jpux8dP4KHPZFkhx3yj-GOD3twv2stebMHu0dDJ7cn8PsnqdRpXWPVr3W97Byc2MUNfa3Vy3kYGTbUGBWs3MiIGDJa1ixIdGiCr9cNDq5a7VZgdcUuScz3Y3bITCgZVQBI0souSfCzbH_PL0LD3i8CQ5bJ6HPJYD48hfPjL2dH07jvwhA7oZNVXFmnJtbbzPm0mnCHeEpdCu6SvCqlzDT1MDNec5maxMjEZinXMq2QWuQTqxx_BjuhDv4FMCW8cqktK42kQnifa5NKI5UWxrjEZBF8HDAorjqzjWK0VW4BKxCwggArVAQHA0pF_941xSZKIng7DuN00yR0M0XX4C2RWqURPO9AHe_GkRJlmNVFoLfgHi8gN-7tkbC4aF25FWVquYzg0xAYm7_1z4fY__9DvIHd6dnpSXHydfb9JTyYtOFKxz8HsLNarv0rJEQr-7qN-j9mSwno
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9QwEB5BEZQLgvIKFDASBx6KSNav5ISqwqq8VgiotLfIcWx1pdYpm91D_wM_mpm8VksFZ1tJ7Pni-SbzZQbgBTLw3Fjn46rkIiYGHZfcidhkSihrjE_aOttfZ-roWHyay3mvf2p6WeVwJrYHdVVb-kaOQTp6SpWJnL_1vSzi2_vpu_NfMXWQokxr307jKlzTAh0dYlvPx-ALvXaXUchFhuGS4EOGM-kKiqYk6cJ4SicyVts-6hLxvKyf_CuJ2vqm6W241ZNKdtCh4A5ccWEPrndtJi_2YPdw6Op2F37_IMU6nXHMn67rZVfNiZ1c0J9bvbSHUfGGGhHCqo2kiCG7Zc2CBIgmuHrd4OCq1XEFVnt2RsK-77MDZkLFKBtA8lZ2RuKfZXs9twgNe7kIDBkno18ng3l1D46nH34eHsV9R4bYCp2sYl9aNSldmVmX-gm3aFupK8FtkvtKykxTPzPjNJepSYxMyizlWqYeaUY-KZXl92En1ME9BKaEUzYtK6-RYAjncm1SaaTSwhibmCyC14MNivOu8EYxllhuDVagwQoyWKEi2B-sVPTvYFNsEBPB83EYt5s2odspmoO3RJqVRvCgM-p4N470KMMILwK9Ze5xAlXm3h4Ji5O2QreiqC2XEbwZgLF5rH8u4tH_F_EMbiDgiy8fZ58fw81Ji1b6ErQPO6vl2j1BbrQqn7ag_wPZtg4d
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Sensitive+fluorescent+hybridisation+protocol+development+for+simultaneous+detection+of+microRNA+and+cellular+marker+proteins+%28in+the+retina%29&rft.jtitle=Histochemistry+and+cell+biology&rft.au=Kov%C3%A1cs-Valasek%2C+Andrea&rft.au=Szalontai%2C+B%C3%A1lint&rft.au=S%C3%A9t%C3%A1l%C3%B3%2C+Gy%C3%B6rgy&rft.au=G%C3%A1briel%2C+Robert&rft.date=2018-11-01&rft.issn=0948-6143&rft.eissn=1432-119X&rft.volume=150&rft.issue=5&rft.spage=557&rft.epage=566&rft_id=info:doi/10.1007%2Fs00418-018-1705-6&rft.externalDBID=n%2Fa&rft.externalDocID=10_1007_s00418_018_1705_6
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0948-6143&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0948-6143&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0948-6143&client=summon