Primary hepatocytes and their cultures in liver apoptosis research

• Published in: Archives of toxicology Vol. 88; no. 2; pp. 199 - 212
• Main Authors: Vinken, Mathieu, Maes, Michaël, Oliveira, André G., Cogliati, Bruno, Marques, Pedro E., Menezes, Gustavo B., Dagli, Maria Lúcia Zaidan, Vanhaecke, Tamara, Rogiers, Vera
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.02.2014; Springer Nature B.V
• Subjects: Anatomy & physiology; Animals; Apoptosis; Apoptosis - drug effects; Biomarkers; Biomarkers - analysis; Biomedical and Life Sciences; Biomedicine; Cell Culture Techniques - methods; Cells, Cultured; Culture Media - pharmacology; Environmental Health; Epidermal Growth Factor - pharmacology; Extracellular Matrix - metabolism; Extracellular Matrix - ultrastructure; Glucagon - pharmacology; Hepatocytes - cytology; Humans; Insulin - pharmacology; Liver; Liver - cytology; Liver Diseases - pathology; Occupational Medicine/Industrial Medicine; Pharmacology/Toxicology; Review Article; Primary hepatocyte; Biomarkers; In vitro culture; Cell death; Apoptosis; Assays

Apoptosis not only plays a key role in physiological demise of defunct hepatocytes, but is also associated with a plethora of acute and chronic liver diseases as well as with hepatotoxicity. The present paper focuses on the modelling of this mode of programmed cell death in primary hepatocyte cultur...