Effects of the application of the amniotic membrane in the healing process of skin wounds in rats

• Published in: Acta cirurgica brasileira Vol. 33; no. 2; pp. 144 - 155
• Main Authors: Campelo, Mariana Barbosa Dias, Santos, Joelita de Alencar Fonseca, Maia Filho, Antonio Luiz Martins, Ferreira, Daniel Cabral Leão, Sant'Anna, Luciana Barros, Oliveira, Rauirys Alencar de, Maia, Leonardo Fonseca, Arisawa, Emilia Ângela Loschiavo
• Format: Journal Article
• Language: English
• Published: Brazil Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 01.02.2018
• Subjects: Amnion; Collagen; Inflammation; Rats; Wound Healing
• ISSN: 0102-8650; 1678-2674
• DOI: 10.1590/s0102-865020180020000006

To evaluate the efficacy of the application of the human amniotic membrane (HAM) on the inflammatory process, fibroblast proliferation, formation of collagenand reduction of skin wound areas in rats. Thirty six rats were submitted to a surgical injury induction and divided into two groups (n = 18): group C (control) and T (treated with the HAM). The macroscopic evolution in the wound area and the histological characteristics of the skin samples were evaluated. The regression of the wound area was greater in group T. The histological analysis revealed a significant reduction (p < 0.05) in the inflammatory infiltrate in group T at all experimental periods compared with that in the control group. Furthermore, the group T presented a significant increase in the proliferation of fibroblasts at 14 and 21 days compared with group C (p < 0.05). Regarding the deposition of mature collagen fibers, there was an increase in the replacement of type III collagen by type I collagen in group T (p < 0.05). Treatment with the HAM reduced the healing time as well as the inflammatory responses, increased the proliferation of fibroblasts, and induced a higher concentration of mature collagen fibers.