Primary application of PPE68 of Mycobacterium tuberculosis

• Published in: Human immunology Vol. 75; no. 5; pp. 428 - 432
• Main Authors: Dong, Zhiling, Xu, Lei, Yang, Jing, He, Yonglin, She, Qian, Wang, Jingxian, Zhang, Zhuangmiao, Feng, Xin, Li, Dairong, Yang, Chun
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.05.2014
• Subjects: Allergy and Immunology; Animals; Antibodies, Bacterial - immunology; Antigens, Bacterial - immunology; Bacterial Proteins - genetics; Bacterial Proteins - immunology; Enzyme-Linked Immunosorbent Assay; Female; Humans; Lung - immunology; Lung - pathology; Male; Mice; Mycobacterium bovis - genetics; Mycobacterium bovis - immunology; Mycobacterium tuberculosis - genetics; Mycobacterium tuberculosis - immunology; Mycobacterium tuberculosis - pathogenicity; Rabbits; Sensitivity and Specificity; Spleen - immunology; Spleen - pathology; Th1 Cells - immunology; Tuberculosis - diagnosis; Tuberculosis - immunology; Virulence - genetics; Virulence - immunology

PPE68 protein is absent from BCG and the attenuated strains of Mycobacterium tuberculosis (MTB). In this study, the shuttle plasmid pBudCE4.1/PPE68/OriM was constructed and transformed into BCG to obtain PPE68 recombination BCG (PPE68-rBCG), and BALB/c mice were immunized with PPE68-rBCG to evaluate...