Transcriptomic profile of cystic fibrosis airway epithelial cells undergoing repair

Pathological remodeling of the airway epithelium is commonly observed in Cystic Fibrosis (CF). The different cell types that constitute the airway epithelium are regenerated upon injury to restore integrity and maintenance of the epithelium barrier function. The molecular signature of tissue repair...

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Bibliographic Details
Published inScientific data Vol. 6; no. 1; pp. 240 - 7
Main Authors Zoso, Alice, Sofoluwe, Aderonke, Bacchetta, Marc, Chanson, Marc
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 29.10.2019
Nature Publishing Group
Subjects
631/337/2019
631/80/85/2361
692/699/1785/3193
Bronchi - cytology
Cells, Cultured
Cystic fibrosis
Cystic Fibrosis - genetics
Data Descriptor
Epithelial cells
Epithelial Cells - metabolism
Epithelium
Flagellin
Humanities and Social Sciences
Humans
multidisciplinary
Respiratory tract
Ribonucleic acid
RNA
Science
Science (multidisciplinary)
Transcriptome
Wound healing
Wounding
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Summary:Pathological remodeling of the airway epithelium is commonly observed in Cystic Fibrosis (CF). The different cell types that constitute the airway epithelium are regenerated upon injury to restore integrity and maintenance of the epithelium barrier function. The molecular signature of tissue repair in CF airway epithelial cells has, however, not well been investigated in primary cultures. We therefore collected RNA-seq data from well-differentiated primary cultures of bronchial human airway epithelial cells (HAECs) of CF (F508del/F508del) and non-CF (NCF) origins before and after mechanical wounding, exposed or not to flagellin. We identified the expression changes with time of repair of genes, the products of which are markers of the different cell types that constitute the airway epithelium (basal, suprabasal, intermediate, secretory, goblet and ciliated cells as well as ionocytes). Researchers in the CF field may benefit from this transcriptomic profile, which covers the initial steps of wound repair and revealed differences in this process between CF and NCF cultures. Measurement(s) messenger RNA Technology Type(s) RNA sequencing Factor Type(s) exposure to flagellin • cystic fibrosis versus non-cystic fibrosis • mechanical wounding Sample Characteristic - Organism Homo sapiens Machine-accessible metadata file describing the reported data: https://doi.org/10.6084/m9.figshare.9944675
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ISSN:2052-4463
2052-4463
DOI:10.1038/s41597-019-0256-6