Different methods of detaching adherent cells and their effects on the cell surface expression of Fas receptor and Fas ligand

• Published in: Scientific reports Vol. 12; no. 1; pp. 5713 - 8
• Main Authors: Lai, Ting-Yu, Cao, Jerry, Ou-Yang, Pu, Tsai, Ching-Yi, Lin, Chih-Wen, Chen, Chien-Chia, Tsai, Meng-Kun, Lee, Chih-Yuan
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 05.04.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 631/1647; 631/337; 631/80; Acetic acid; Adherent cells; Apoptosis; Buffers; Cell culture; Cell Culture Techniques; Cell surface; Extracellular matrix; Fas Ligand Protein; fas Receptor; FasL protein; Humanities and Social Sciences; Ligands; multidisciplinary; Proteins; Science; Science (multidisciplinary); Trypsin; Trypsin - metabolism

In cell culture environment, some cells adhere firmly to the culture plates and may be vulnerable to cell detachment during passage. Therefore, it is important to harvest cells with a proper detaching method to maintain the viability of cells after detachment. Trypsinization is frequently used for c...