Sphingolipid Domains in the Plasma Membranes of Fibroblasts Are Not Enriched with Cholesterol

• Published in: The Journal of biological chemistry Vol. 288; no. 23; pp. 16855 - 16861
• Main Authors: Frisz, Jessica F., Klitzing, Haley A., Lou, Kaiyan, Hutcheon, Ian D., Weber, Peter K., Zimmerberg, Joshua, Kraft, Mary L.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 07.06.2013; American Society for Biochemistry and Molecular Biology
• Subjects: Animals; BASIC BIOLOGICAL SCIENCES; Cholesterol; Cholesterol - metabolism; Cytoskeleton - metabolism; Fibroblasts - cytology; Fibroblasts - metabolism; INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; Isotopic Tracers; Lipid Raft; Lipids; Mass Spectrometry (MS); Membrane Microdomains - metabolism; Membrane Structure; Mice; NIH 3T3 Cells; Plasma Membrane; Sphingolipid; Sphingolipids - metabolism; Lipid Raft; Membrane Structure; Plasma Membrane; Mass Spectrometry (MS); Sphingolipid; Isotopic Tracers; Cholesterol

The plasma membranes of mammalian cells are widely expected to contain domains that are enriched with cholesterol and sphingolipids. In this work, we have used high-resolution secondary ion mass spectrometry to directly map the distributions of isotope-labeled cholesterol and sphingolipids in the pl...