Expression and immunolocalization of metallothioneins MT1, MT2 and MT3 in rat nephron

• Published in: Journal of trace elements in medicine and biology Vol. 46; pp. 62 - 75
• Main Authors: Sabolić, Ivan, Škarica, Mario, Ljubojević, Marija, Breljak, Davorka, Herak-Kramberger, Carol M., Crljen, Vladiana, Ljubešić, Nikola
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01.03.2018
• Subjects: Animals; Cell Nucleus - metabolism; Cytoplasm - metabolism; Immunocytochemistry; Immunohistochemistry; Male; Metallothionein - metabolism; Microscopy, Electron, Transmission; mRNA expression; Nephrons - metabolism; Nerve Tissue Proteins - metabolism; Proximal tubule; Rat kidney; Rats, Wistar; Transmission electron microscopy; Western blotting; Proximal tubule; Transmission electron microscopy; Immunocytochemistry; Rat kidney; Western blotting; mRNA expression

Rodent kidneys exhibit three isoforms of metallothioneins (MTs), MT1, MT2 and MT3, with poorly characterized localization along the nephron. Here we studied in adult male Wistar rats the renal expression of MTs mRNA by end-point RT-PCR and MT proteins by immunochemical methods The expression pattern...