DA-9601 Decreases Immediate-Type Allergic Reaction and Tumor Necrosis Factor-α Production

• Published in: Journal of Health Science Vol. 52; no. 4; pp. 383 - 389
• Main Authors: Kim, Sug-Hyun, Moon, Jin-Young, Jun, Chang-Duk, Kim, Cheorl-Ho, Oh, Tae-Young, Chung, Jae Myung, Lee, Soyoung, Kim, Sang-Hyun, Park, Won-Hwan, Park, Hyo-Hyun
• Format: Journal Article
• Language: English
• Published: The Pharmaceutical Society of Japan 2006
• Subjects: DA-9601; immediate-type allergic reaction; mast cells; tumor necrosis factor-α
• ISSN: 1344-9702; 1347-5207
• DOI: 10.1248/jhs.52.383

The immediate-type allergic reaction is involved in many allergic diseases such as asthma, allergic rhinitis, and sinusitis. The discovery of drugs for the treatment of immediate-type allergic disease is a very important subject in human health. The formulated ethanol extract of Artemisia asiatica Nakai (DA-9601) has been reported to have anti-oxidative and anti-inflammatory activities. In this report, we investigated the effect of DA-9601 on the immediate-type allergic reaction and studied its possible mechanisms of action, focusing on the mast cell-mediated allergic reaction. DA-9601 inhibited compound 48/80-induced systemic anaphylactic reactions and serum histamine release in mice. DA-9601 decreased the IgE-mediated passive cutaneous anaphylaxis, the model of local allergic reaction in vivo. DA-9601 dose-dependently reduced histamine release from mast cells activated by compound 48/80 or IgE. Furthermore, DA-9601 decreased the gene expression and production of tumor necrosis factor (TNF)-α in phorbol 12-myristate 13-acetate plus A23187-stimulated mast cells. These findings provide evidence that DA-9601 could be a candidate as an anti-allergic agent.