An inoculum-dependent culturing strategy (IDC) for the cultivation of environmental microbiomes and the isolation of novel endophytic Actinobacteria
The recent introduction of plant-only-based culture media enabled cultivation of not-yet-cultured bacteria that exceed 90% of the plant microbiota communities. Here, we further prove the competence and challenge of such culture media, and further introduce “the inoculum-dependent culturing strategy,...
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Published in | Journal of antibiotics Vol. 73; no. 1; pp. 66 - 71 |
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Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
London
Nature Publishing Group UK
01.01.2020
Nature Publishing Group |
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Abstract | The recent introduction of plant-only-based culture media enabled cultivation of not-yet-cultured bacteria that exceed 90% of the plant microbiota communities. Here, we further prove the competence and challenge of such culture media, and further introduce “the inoculum-dependent culturing strategy, IDC”. The strategy depends on direct inoculating plant serial dilutions onto plain water agar plates, allowing bacteria to grow only on the expense of natural nutrients contained in the administered inoculum. Developed colonies are successively transferred/subcultured onto plant-only-based culture media, which contains natural nutrients very much alike to those found in the prepared plant inocula. Because of its simplicity, the method is recommended as a powerful tool in screening programs that require microbial isolation from a large number of diverse plants. Here, the method comfortably and successfully recovered several isolates of endophytic Actinobacteria represented by the six genera of
Curtobacterium
spp.,
Plantibacter
spp.,
Agreia
spp.,
Herbiconiux
spp.,
Rhodococcus
spp., and
Nocardioides
spp. Furthermore, two of the isolates are most likely novel species belonging to
Agreia
spp. and
Herbiconiux
spp. |
---|---|
AbstractList | The recent introduction of plant-only-based culture media enabled cultivation of not-yet-cultured bacteria that exceed 90% of the plant microbiota communities. Here, we further prove the competence and challenge of such culture media, and further introduce “the inoculum-dependent culturing strategy, IDC”. The strategy depends on direct inoculating plant serial dilutions onto plain water agar plates, allowing bacteria to grow only on the expense of natural nutrients contained in the administered inoculum. Developed colonies are successively transferred/subcultured onto plant-only-based culture media, which contains natural nutrients very much alike to those found in the prepared plant inocula. Because of its simplicity, the method is recommended as a powerful tool in screening programs that require microbial isolation from a large number of diverse plants. Here, the method comfortably and successfully recovered several isolates of endophytic Actinobacteria represented by the six genera of Curtobacterium spp., Plantibacter spp., Agreia spp., Herbiconiux spp., Rhodococcus spp., and Nocardioides spp. Furthermore, two of the isolates are most likely novel species belonging to Agreia spp. and Herbiconiux spp. Abstract The recent introduction of plant-only-based culture media enabled cultivation of not-yet-cultured bacteria that exceed 90% of the plant microbiota communities. Here, we further prove the competence and challenge of such culture media, and further introduce “the inoculum-dependent culturing strategy, IDC”. The strategy depends on direct inoculating plant serial dilutions onto plain water agar plates, allowing bacteria to grow only on the expense of natural nutrients contained in the administered inoculum. Developed colonies are successively transferred/subcultured onto plant-only-based culture media, which contains natural nutrients very much alike to those found in the prepared plant inocula. Because of its simplicity, the method is recommended as a powerful tool in screening programs that require microbial isolation from a large number of diverse plants. Here, the method comfortably and successfully recovered several isolates of endophytic Actinobacteria represented by the six genera of Curtobacterium spp., Plantibacter spp., Agreia spp., Herbiconiux spp., Rhodococcus spp., and Nocardioides spp. Furthermore, two of the isolates are most likely novel species belonging to Agreia spp. and Herbiconiux spp. The recent introduction of plant-only-based culture media enabled cultivation of not-yet-cultured bacteria that exceed 90% of the plant microbiota communities. Here, we further prove the competence and challenge of such culture media, and further introduce “the inoculum-dependent culturing strategy, IDC”. The strategy depends on direct inoculating plant serial dilutions onto plain water agar plates, allowing bacteria to grow only on the expense of natural nutrients contained in the administered inoculum. Developed colonies are successively transferred/subcultured onto plant-only-based culture media, which contains natural nutrients very much alike to those found in the prepared plant inocula. Because of its simplicity, the method is recommended as a powerful tool in screening programs that require microbial isolation from a large number of diverse plants. Here, the method comfortably and successfully recovered several isolates of endophytic Actinobacteria represented by the six genera of Curtobacterium spp., Plantibacter spp., Agreia spp., Herbiconiux spp., Rhodococcus spp., and Nocardioides spp. Furthermore, two of the isolates are most likely novel species belonging to Agreia spp. and Herbiconiux spp. |
Author | Sarhan, Mohamed S. Mourad, Elhussein F. Ruppel, Silke Abdelfadeel, Mohamed R. Daanaa, Hassan-Sibroe A. Youssef, Hanan H. Eichler-Löbermann, Bettina Hegazi, Nabil A. Nemr, Rahma A. Goda, Hanan A. Hamza, Mervat A. Fayez, Mohamed |
Author_xml | – sequence: 1 givenname: Mohamed S. orcidid: 0000-0003-0904-976X surname: Sarhan fullname: Sarhan, Mohamed S. organization: Department of Microbiology, Faculty of Agriculture, Cairo University, Faculty of Agricultural and Environmental Sciences, Rostock University – sequence: 2 givenname: Elhussein F. orcidid: 0000-0001-6247-7769 surname: Mourad fullname: Mourad, Elhussein F. organization: Department of Microbiology, Faculty of Agriculture, Cairo University – sequence: 3 givenname: Rahma A. surname: Nemr fullname: Nemr, Rahma A. organization: Department of Microbiology, Faculty of Agriculture, Cairo University – sequence: 4 givenname: Mohamed R. surname: Abdelfadeel fullname: Abdelfadeel, Mohamed R. organization: Department of Microbiology, Faculty of Agriculture, Cairo University – sequence: 5 givenname: Hassan-Sibroe A. surname: Daanaa fullname: Daanaa, Hassan-Sibroe A. organization: Department of Genetics, School of Life Science, the Graduate University for Advanced Studies (SOKENDAI), 1111 Yata – sequence: 6 givenname: Hanan H. surname: Youssef fullname: Youssef, Hanan H. organization: Department of Microbiology, Faculty of Agriculture, Cairo University – sequence: 7 givenname: Hanan A. surname: Goda fullname: Goda, Hanan A. organization: Department of Microbiology, Faculty of Agriculture, Cairo University – sequence: 8 givenname: Mervat A. surname: Hamza fullname: Hamza, Mervat A. organization: Department of Microbiology, Faculty of Agriculture, Cairo University – sequence: 9 givenname: Mohamed surname: Fayez fullname: Fayez, Mohamed organization: Department of Microbiology, Faculty of Agriculture, Cairo University – sequence: 10 givenname: Bettina surname: Eichler-Löbermann fullname: Eichler-Löbermann, Bettina organization: Faculty of Agricultural and Environmental Sciences, Rostock University – sequence: 11 givenname: Silke surname: Ruppel fullname: Ruppel, Silke organization: Leibniz Institute of Vegetable and Ornamental Crops (IGZ) – sequence: 12 givenname: Nabil A. surname: Hegazi fullname: Hegazi, Nabil A. email: hegazinabil8@gmail.com organization: Department of Microbiology, Faculty of Agriculture, Cairo University |
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CitedBy_id | crossref_primary_10_3390_d15010046 crossref_primary_10_3390_molecules28227512 crossref_primary_10_3389_fmicb_2020_00454 crossref_primary_10_3389_fsufs_2021_660790 crossref_primary_10_1021_acs_jafc_3c08885 crossref_primary_10_1007_s11105_023_01429_y crossref_primary_10_1111_nph_17319 |
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Title | An inoculum-dependent culturing strategy (IDC) for the cultivation of environmental microbiomes and the isolation of novel endophytic Actinobacteria |
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