Engineering the AAVS1 locus for consistent and scalable transgene expression in human iPSCs and their differentiated derivatives - Oceguera-Yanez, Fabian; Kim, Shin-Il; Matsumoto, Tomoko; Tan, Ghee Wan; Xiang, Long; Hatani, Takeshi; Kondo, Takayuki; Ikeya, Makoto; Yoshida, Yoshinori; Inoue, Haruhisa; Woltjen, Knut (Methods (San Diego, Calif.), 2016)
Keywords: AAVS1; Base Sequence; Cell Differentiation; Cell Line; Chromosomes, Human, Pair 19 - genetics; Cloning, Molecular; CRISPR-Cas Systems; CRISPR/Cas9; drugs; engineering; epigenetics; gene expression; Gene targeting; Genes, Reporter; genetic background; Genetic Engineering; Genetic Loci; Genetic Vectors; genetically modified organisms; genotyping; Green Fluorescent Proteins - biosynthesis; Green Fluorescent Proteins - genetics; Human induced pluripotent stem cell (iPSC); Humans; in vitro culture; induced pluripotent stem cells; Induced Pluripotent Stem Cells - physiology; loci; medicine; Nuclease; Parvovirinae - genetics; phenotype; Promoter Regions, Genetic; TALEN; Transfection; Transgenes; transposons; viruses; Gene targeting; Nuclease; Human induced pluripotent stem cell (iPSC); CRISPR/Cas9; AAVS1; TALEN