Nucleic acid testing and molecular characterization of HIV infections

Significant advances have been made in the molecular assays used for the detection of human immunodeficiency virus (HIV), which are crucial in preventing HIV transmission and monitoring disease progression. Molecular assays for HIV diagnosis have now reached a high degree of specificity, sensitivity...

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Published inEuropean Journal of Clinical Microbiology & Infectious Diseases Vol. 38; no. 5; pp. 829 - 842
Main Authors Zhao, Junpeng, Chang, Le, Wang, Lunan
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer Science and Business Media LLC 01.05.2019
Springer Berlin Heidelberg
Springer Nature B.V
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Abstract Significant advances have been made in the molecular assays used for the detection of human immunodeficiency virus (HIV), which are crucial in preventing HIV transmission and monitoring disease progression. Molecular assays for HIV diagnosis have now reached a high degree of specificity, sensitivity and reproducibility, and have less operator involvement to minimize risk of contamination. Furthermore, analyses have been developed for the characterization of host gene polymorphisms and host responses to better identify and monitor HIV-1 infections in the clinic. Currently, molecular technologies including HIV quantitative and qualitative assays are mainly based on the polymerase chain reaction (PCR), transcription-mediated amplification (TMA), nucleic acid sequence-based amplification (NASBA), and branched chain (b) DNA methods and widely used for HIV detection and characterization, such as blood screening, point-of-care testing (POCT), pediatric diagnosis, acute HIV infection (AHI), HIV drug resistance testing, antiretroviral (AR) susceptibility testing, host genome polymorphism testing, and host response analysis. This review summarizes the development and the potential utility of molecular assays used to detect and characterize HIV infections.
AbstractList Significant advances have been made in the molecular assays used for the detection of human immunodeficiency virus (HIV), which are crucial in preventing HIV transmission and monitoring disease progression. Molecular assays for HIV diagnosis have now reached a high degree of specificity, sensitivity and reproducibility, and have less operator involvement to minimize risk of contamination. Furthermore, analyses have been developed for the characterization of host gene polymorphisms and host responses to better identify and monitor HIV-1 infections in the clinic. Currently, molecular technologies including HIV quantitative and qualitative assays are mainly based on the polymerase chain reaction (PCR), transcription-mediated amplification (TMA), nucleic acid sequence-based amplification (NASBA), and branched chain (b) DNA methods and widely used for HIV detection and characterization, such as blood screening, point-of-care testing (POCT), pediatric diagnosis, acute HIV infection (AHI), HIV drug resistance testing, antiretroviral (AR) susceptibility testing, host genome polymorphism testing, and host response analysis. This review summarizes the development and the potential utility of molecular assays used to detect and characterize HIV infections.
Significant advances have been made in the molecular assays used for the detection of human immunodeficiency virus (HIV), which are crucial in preventing HIV transmission and monitoring disease progression. Molecular assays for HIV diagnosis have now reached a high degree of specificity, sensitivity and reproducibility, and have less operator involvement to minimize risk of contamination. Furthermore, analyses have been developed for the characterization of host gene polymorphisms and host responses to better identify and monitor HIV-1 infections in the clinic. Currently, molecular technologies including HIV quantitative and qualitative assays are mainly based on the polymerase chain reaction (PCR), transcription-mediated amplification (TMA), nucleic acid sequence-based amplification (NASBA), and branched chain (b) DNA methods and widely used for HIV detection and characterization, such as blood screening, point-of-care testing (POCT), pediatric diagnosis, acute HIV infection (AHI), HIV drug resistance testing, antiretroviral (AR) susceptibility testing, host genome polymorphism testing, and host response analysis. This review summarizes the development and the potential utility of molecular assays used to detect and characterize HIV infections.Significant advances have been made in the molecular assays used for the detection of human immunodeficiency virus (HIV), which are crucial in preventing HIV transmission and monitoring disease progression. Molecular assays for HIV diagnosis have now reached a high degree of specificity, sensitivity and reproducibility, and have less operator involvement to minimize risk of contamination. Furthermore, analyses have been developed for the characterization of host gene polymorphisms and host responses to better identify and monitor HIV-1 infections in the clinic. Currently, molecular technologies including HIV quantitative and qualitative assays are mainly based on the polymerase chain reaction (PCR), transcription-mediated amplification (TMA), nucleic acid sequence-based amplification (NASBA), and branched chain (b) DNA methods and widely used for HIV detection and characterization, such as blood screening, point-of-care testing (POCT), pediatric diagnosis, acute HIV infection (AHI), HIV drug resistance testing, antiretroviral (AR) susceptibility testing, host genome polymorphism testing, and host response analysis. This review summarizes the development and the potential utility of molecular assays used to detect and characterize HIV infections.
Author Le Chang
Junpeng Zhao
Lunan Wang
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  organization: National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Beijing Engineering Research Center of Laboratory Medicine, Beijing Hospital, Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences
BackLink https://cir.nii.ac.jp/crid/1871709542497864320$$DView record in CiNii
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Qualitative nucleic acid assays
Human immunodeficiency virus
Nucleic acid testing
Quantitative nucleic assays
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PublicationTitle European Journal of Clinical Microbiology & Infectious Diseases
PublicationTitleAbbrev Eur J Clin Microbiol Infect Dis
PublicationTitleAlternate Eur J Clin Microbiol Infect Dis
PublicationYear 2019
Publisher Springer Science and Business Media LLC
Springer Berlin Heidelberg
Springer Nature B.V
Publisher_xml – name: Springer Science and Business Media LLC
– name: Springer Berlin Heidelberg
– name: Springer Nature B.V
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Snippet Significant advances have been made in the molecular assays used for the detection of human immunodeficiency virus (HIV), which are crucial in preventing HIV...
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SubjectTerms Amplification
Antiretroviral agents
Assaying
Biomedical and Life Sciences
Biomedicine
Chain branching
Contamination
Deoxyribonucleic acid
Diagnosis
Disease transmission
DNA
Drug resistance
Genomes
Genotype
HIV
HIV Infections
HIV-1
Host-Pathogen Interactions
Human immunodeficiency virus
Humans
Infections
Internal Medicine
Medical Microbiology
Microbial Sensitivity Tests
Nucleic Acid Amplification Techniques
Nucleic acids
Nucleotide sequence
Point-of-Care Testing
Polymerase chain reaction
Polymorphism
Reagent Kits, Diagnostic
Reproducibility
Review
Risk reduction
RNA, Viral
Transcription
Viruses
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Title Nucleic acid testing and molecular characterization of HIV infections
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