Substrate-Dependent Functional Alterations of Seven CYP2C9 Variants Found in Japanese Subjects

• Published in: Drug metabolism and disposition Vol. 37; no. 9; pp. 1895 - 1903
• Main Authors: Maekawa, Keiko, Harakawa, Noriko, Sugiyama, Emiko, Tohkin, Masahiro, Kim, Su-Ryang, Kaniwa, Nahoko, Katori, Noriko, Hasegawa, Ryuichi, Yasuda, Kazuki, Kamide, Kei, Miyata, Toshiyuki, Saito, Yoshiro, Sawada, Jun-ichi
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 01.09.2009; American Society for Pharmacology and Experimental Therapeutics
• Subjects: Alleles; Angiotensin II Type 1 Receptor Blockers - metabolism; Animals; Biological and medical sciences; Blotting, Western; Cytochrome P-450 Enzyme System - genetics; Cytochrome P-450 Enzyme System - metabolism; Diclofenac - metabolism; DNA, Complementary - biosynthesis; DNA, Complementary - genetics; Genetic Variation; Humans; Hypoglycemic Agents - metabolism; Insecta; Japan; Kinetics; Losartan - metabolism; Medical sciences; Microsomes - metabolism; Pharmacology. Drug treatments; Rats; Recombinant Proteins - metabolism; Substrate Specificity; Sulfonylurea Compounds - metabolism; Asia; Japan; Human; Substrate; Variant; Genetic variability; Enzyme; Isozyme; Cytochrome P450; Genotype; CYP2C9 gene
• ISSN: 0090-9556; 1521-009X; 1521-009X
• DOI: 10.1124/dmd.109.027003

CYP2C9 is a polymorphic enzyme that metabolizes a number of clinically important drugs. In this study, catalytic activities of seven alleles found in Japanese individuals, CYP2C9*3 (I359L), *13 (L90P), *26 (T130R), *28 (Q214L), *30 (A477T), *33 (R132Q), and *34 (R335Q), were assessed using three substrates (diclofenac, losartan, and glimepiride). When expressed in a baculovirus-insect cell system, the holo and total (apo and holo) CYP2C9 protein expression levels were similar among the wild type (CYP2C9.1) and six variants except for CYP2C9.13. A large part of CYP2C9.13 was present in the apo form P420. Compared with CYP2C9.1, all variants except for CYP2C9.34 exhibited substrate-dependent changes in Km, Vmax, and intrinsic clearance (Vmax/Km). For diclofenac 4′-hydroxylation, the intrinsic clearance was decreased markedly (by >80%) in CYP2C9.13, CYP2C9.30, and CYP2C9.33 and variably (63–76%) in CYP2C9.3, CYP2C9.26, and CYP2C9.28 due to increased Km and/or decreased Vmax values. For losartan oxidation, CYP2C9.13 and CYP2C9.28 showed 2.5- and 1.8-fold higher Km values, respectively, and all variants except for CYP2C9.34 showed >77% lower Vmax and intrinsic clearance values. For glimepiride hydroxylation, the Km of CYP2C9.13 was increased 7-fold, and the Vmax values of all variants significantly decreased, resulting in reductions in the intrinsic clearance by >80% in CYP2C9.3, CYP2C9.13, CYP2C9.26, and CYP2C9.33 and by 56 to 75% in CYP2C9.28 and CYP2C9.30. These findings suggest the necessity for careful administration of losartan and glimepiride to patients bearing these six alleles.