The role of separation science in proteomics research

• Published in: Electrophoresis Vol. 22; no. 17; pp. 3629 - 3638
• Main Author: Issaq, Haleem J.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.09.2001
• Subjects: Chromatography, High Pressure Liquid - methods; Electrophoresis, Capillary - methods; Isotope-coded affinity tags; Multidimensional; Peptides; Proteins; Proteins - isolation & purification; Proteome; Proteome - isolation & purification; Reproducibility of Results; Review

In the last few years there has been an increased effort into the separation, quantification and identification of all proteins in a cell or tissue. This is a review of the role gel electrophoresis, high performance liquid chromatography (HPLC), and capillary electrophoresis (CE) play in proteomics...