Long‐read DNA metabarcoding of ribosomal RNA in the analysis of fungi from aquatic environments

• Published in: Molecular ecology resources Vol. 18; no. 6; pp. 1500 - 1514
• Main Authors: Heeger, Felix, Bourne, Elizabeth C., Baschien, Christiane, Yurkov, Andrey, Bunk, Boyke, Spröer, Cathrin, Overmann, Jörg, Mazzoni, Camila J., Monaghan, Michael T.
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.11.2018
• Subjects: aquatic; Aquatic environment; Aquatic fungi; Aquatic Organisms - classification; Aquatic Organisms - genetics; Base pairs; Bioinformatics; chimera formation; Chimeras; chimerism; Cluster Analysis; Deoxyribonucleic acid; DNA; DNA barcoding; DNA Barcoding, Taxonomic - methods; DNA sequencing; DNA, Fungal - chemistry; DNA, Fungal - genetics; DNA, Ribosomal Spacer - chemistry; DNA, Ribosomal Spacer - genetics; eukaryotic cells; Fungi; Fungi - classification; Fungi - genetics; genetic databases; internal transcribed spacers; lakes; metabarcoding; Metagenomics - methods; Microorganisms; mock community; operon; PacBio; Phylogeny; Ribonucleic acid; ribosomal RNA; RNA; RNA, Fungal - genetics; RNA, Ribosomal - genetics; RNA, Ribosomal, 18S - genetics; rRNA; Sediment samplers; sediments; Sequence Analysis, DNA; Taxonomy; mock community; chimera formation; metabarcoding; aquatic; fungi; PacBio

DNA metabarcoding is widely used to study prokaryotic and eukaryotic microbial diversity. Technological constraints limit most studies to marker lengths below 600 base pairs (bp). Longer sequencing reads of several thousand bp are now possible with third‐generation sequencing. Increased marker lengt...