Novel Alginate-Chitosan Composite Microspheres for Implant Delivery of Vancomycin and In Vivo Evaluation
In this study, vancomycin loaded alginate–chitosan composite microspheres were developed by emulsion cross‐linking method. The in vitro and vivo characterizations were done to evaluate the feasibility of application. Our experimental results showed that the emulsification cross‐linking technique app...
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Published in | Chemical biology & drug design Vol. 88; no. 3; pp. 434 - 440 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
England
Blackwell Publishing Ltd
01.09.2016
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Subjects | |
Online Access | Get full text |
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Summary: | In this study, vancomycin loaded alginate–chitosan composite microspheres were developed by emulsion cross‐linking method. The in vitro and vivo characterizations were done to evaluate the feasibility of application. Our experimental results showed that the emulsification cross‐linking technique appeared to be a feasible method for the preparation of alginate–chitosan composite microspheres. The microspheres were spherical in shape and the mean particle size and drug loading were 25.3 ± 5.4 μm and 18.5 ± 2.3% respectively. A sustained vancomycin release was realized i.e. the amount of cumulative release increased in a time frame of 24 h to reach an amount i.e. ~68%. The model that fit best for vancomycin released from the microspheres was the Higuchi kinetic model with a correlation coefficient r = 0.9996. In vivo results showed that the application of microspheres not only reduced the toxicity, but also maintained effective drug concentration. In addition, no severe signs of epithelial necrosis and sloughing of epithelial cells were detected in histological studies.
Vancomycin loaded alginate–chitosan composite microspheres were developed by emulsion cross‐linking method. The microspheres were spherical in shape and the mean particle size and drug loading were 25.3 ± 5.4 µm and 18.5 ± 2.3%. In vivo results showed that the application of microspheres not only reduced the toxicity, but also maintained effective drug concentration. |
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Bibliography: | ArticleID:CBDD12771 National Natural Science Foundation of China - No. 81370107 ark:/67375/WNG-1Q6ZW3FD-Z istex:326985FD4E089EF9598F776743086882D679A2DB Shanghai Municipal Science and Technology Commission - No. 134119b1002 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1747-0277 1747-0285 |
DOI: | 10.1111/cbdd.12771 |