Spontaneous In Vivo Chondrogenesis of Bone Marrow‐Derived Mesenchymal Progenitor Cells by Blocking Vascular Endothelial Growth Factor Signaling

• Published in: Stem cells translational medicine Vol. 5; no. 12; pp. 1730 - 1738
• Main Authors: Marsano, Anna, Medeiros da Cunha, Carolina M., Ghanaati, Shahram, Gueven, Sinan, Centola, Matteo, Tsaryk, Roman, Barbeck, Mike, Stuedle, Chiara, Barbero, Andrea, Helmrich, Uta, Schaeren, Stefan, Kirkpatrick, James C., Banfi, Andrea, Martin, Ivan
• Format: Journal Article
• Language: English
• Published: Durham, NC, USA AlphaMed Press 01.12.2016; Oxford University Press
• Subjects: Adult; Angiogenesis; Bone marrow; Bone Marrow Cells - cytology; Bone Marrow Cells - drug effects; Bone Marrow Cells - metabolism; Cartilage; Cell Differentiation - drug effects; Cell Proliferation - drug effects; Chondrogenesis; Chondrogenesis - drug effects; Data analysis; Endothelial Cells - cytology; Endothelial Cells - drug effects; Endothelial Cells - metabolism; Enzymes; Female; Humans; Hypertrophy; Hypoxia; Laboratory animals; Male; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - drug effects; Mesenchymal Stromal Cells - metabolism; Mesenchymal stromal/stem cells; Mesenchyme; Neovascularization, Physiologic - drug effects; Osteoprogenitor cells; Oxygen - pharmacology; Phenotypes; Progenitor cells; Signal Transduction - drug effects; Stem cell transplantation; Stem cells; Tissue Engineering and Regenerative Medicine; Transduction, Genetic; Transforming Growth Factor beta - metabolism; Vascular endothelial growth factor; Vascular Endothelial Growth Factor A - metabolism; Vascular endothelial growth factor blockade; Vascular Endothelial Growth Factor Receptor-2 - metabolism; Young Adult; Hypoxia; Chondrogenesis; Mesenchymal stromal/stem cells; Vascular endothelial growth factor blockade

This study assessed whether simple inhibition of angiogenesis by vascular endothelial growth factor (VEGF) blockade is sufficient to direct in vivo chondrogenic differentiation of implanted human mesenchymal stromal/stem cells (MSCs). MSCs transduced to express sFlk‐1 and directly implanted subcutan...