Mettl1/Wdr4-Mediated m7G tRNA Methylome Is Required for Normal mRNA Translation and Embryonic Stem Cell Self-Renewal and Differentiation

• Published in: Molecular cell Vol. 71; no. 2; pp. 244 - 255.e5
• Main Authors: Lin, Shuibin, Liu, Qi, Lelyveld, Victor S., Choe, Junho, Szostak, Jack W., Gregory, Richard I.
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 19.07.2018
• Subjects: abnormal development; brain; cell cycle; codons; dwarfing; embryonic stem cells; human diseases; m7G; MeRIP-seq; messenger RNA; methylation; methyltransferases; Mettl1; mice; mutation; N7-methylguanosine; neural development; precipitin tests; ribosomes; RNA methylation; TRAC-seq; transfer RNA; translation; translation (genetics); tRNA; Wdr4; TRAC-seq; tRNA; embryonic stem cells; Wdr4; Mettl1; N7-methylguanosine; translation; MeRIP-seq; neural development; m7G; RNA methylation

tRNAs are subject to numerous modifications, including methylation. Mutations in the human N7-methylguanosine (m7G) methyltransferase complex METTL1/WDR4 cause primordial dwarfism and brain malformation, yet the molecular and cellular function in mammals is not well understood. We developed m7G meth...