Enhancement of the yield of long helical structure of recombinant nucleocapsid protein of Newcastle disease virus

• Published in: Process biochemistry (1991) Vol. 48; no. 2; pp. 267 - 271
• Main Authors: Yap, Chee Fai, Tan, Wen Siang, Sieo, Chin Chin, Tey, Beng Ti
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.02.2013
• Subjects: Avian orthoavulavirus 1; computer software; EDTA (chelating agent); Endogenous protease; Escherichia coli; image analysis; mutants; Newcastle disease virus; Nucleocapsid protein; nucleocapsid proteins; PeptideCutter; Protease inhibitor; proteinase inhibitors; proteolysis; serine proteinases; synergism; Western blotting; Newcastle disease virus; Endogenous protease; Nucleocapsid protein; Escherichia coli; PeptideCutter; Protease inhibitor

► Western blot image analysis was used to predict proteases cleavage sites ofNPΔc375. ► PeptideCutter program was used to identify the protease that attacking NPΔc375. ► Metallo- and serine-proteases were predicted as the protease that attacking NPΔc375. ► EDTA and PMSF were used to inhibit activiti...