The main protease 3CLpro of the SARS-CoV-2 virus: how to turn an enemy into a helper

• Published in: Frontiers in bioengineering and biotechnology Vol. 11; p. 1187761
• Main Authors: Belenkaya, Svetlana V., Merkuleva, Iuliia A., Yarovaya, Olga I., Chirkova, Varvara Yu, Sharlaeva, Elena A., Shanshin, Daniil V., Volosnikova, Ekaterina A., Vatsadze, Sergey Z., Khvostov, Mikhail V., Salakhutdinov, Nariman F., Shcherbakov, Dmitriy N.
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 29.06.2023
• Subjects: 3CL protease; Bioengineering and Biotechnology; E. coli bacteria; RBD; SARS-CoV-2; TEV protease; 3CL protease; SARS-CoV-2; RBD; E. coli bacteria; TEV protease

Despite the long history of use and the knowledge of the genetics and biochemistry of E. coli , problems are still possible in obtaining a soluble form of recombinant proteins in this system. Although, soluble protein can be obtained both in the cytoplasm and in the periplasm of the bacterial cell....