Triton X-114 phase separation in the isolation and purification of mouse liver microsomal membrane proteins

• Published in: Methods (San Diego, Calif.) Vol. 54; no. 4; pp. 396 - 406
• Main Authors: Mathias, Rommel A., Chen, Yuan-Shou, Kapp, Eugene A., Greening, David W., Mathivanan, Suresh, Simpson, Richard J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.08.2011
• Subjects: Animals; Chemical Fractionation - methods; Cytochrome P450; Detergent; Liver; Mass spectrometry; Membrane; Membrane Proteins - isolation & purification; Mice; Microsomes; Microsomes, Liver - metabolism; Polyethylene Glycols - chemistry; Proteome; Proteomics; Triton X-114; IPI; Liver; DP; TMHMM; LC–MS/MS; TX-114; CMC; MF; Membrane; CYP; Detergent; Cytochrome P450; 2-D; TMD; 3-D; IMP; ER; MGF; Microsomes; AP; PF; GRAVY; Proteomics; Triton X-114; Mass spectrometry

Integral membrane proteins (IMPs) mediate several cellular functions including cell adhesion, ion and nutrient transport, and cell signalling. IMPs are typically hard to isolate and purify due to their hydrophobic nature and low cellular abundance, however, microsomes are small lipid vesicles rich i...