Calcineurin inhibitors stimulate Kir4.1/Kir5.1 of the distal convoluted tubule to increase NaCl cotransporter

• Published in: JCI insight Vol. 8; no. 7
• Main Authors: Zhang, Dan-Dan, Duan, Xin-Peng, Mutig, Kerim, Rausch, Franziska, Xiao, Yu, Zheng, Jun-Ya, Lin, Dao-Hong, Wang, Wen-Hui
• Format: Journal Article
• Language: English
• Published: United States American Society for Clinical Investigation 10.04.2023; American Society for Clinical investigation
• Subjects: Animals; Calcineurin Inhibitors - pharmacology; Cell biology; Mice; Mice, Knockout; Nephrology; Sodium Chloride - metabolism; Solute Carrier Family 12, Member 3 - metabolism; Tacrolimus - pharmacology; Tacrolimus Binding Protein 1A - metabolism; Thiazides; Hypertension; Nephrology; Potassium channels; Cell Biology

We examine whether calcineurin or protein phosphatase 2B (PP2B) regulates the basolateral inwardly rectifying potassium channel Kir4.1/Kir5.1 in the distal convoluted tubule (DCT). Application of tacrolimus (FK506) or cyclosporine A (CsA) increased whole-cell Kir4.1/Kir5.1-mediated K+ currents and h...