Ultrasensitive detection of aggregated α-synuclein using quiescent seed amplification assay for the diagnosis of Parkinson’s disease

• Published in: Translational neurodegeneration Vol. 13; no. 1; pp. 35 - 16
• Main Authors: Mao, Hengxu, Kuang, Yaoyun, Feng, Du, Chen, Xiang, Lu, Lin, Xia, Wencheng, Gan, Tingting, Huang, Weimeng, Guo, Wenyuan, Yi, Hancun, Yang, Yirong, Wu, Zhuohua, Dai, Wei, Sun, Hui, Wu, Jieyuan, Zhang, Rui, Zhang, Shenqing, Lin, Xiuli, Yong, Yuxuan, Yang, Xinling, Li, Hongyan, Wu, Wenjun, Huang, Xiaoyun, Bian, Zhaoxiang, Wong, Hoi Leong Xavier, Wang, Xin-Lu, Poppell, Michael, Ren, Yi, Liu, Cong, Zou, Wen-Quan, Chen, Shengdi, Xu, Ping-Yi
• Format: Journal Article
• Language: English
• Published: England BioMed Central 24.07.2024; BMC
• Subjects: Aged; Aged, 80 and over; alpha-Synuclein - genetics; alpha-Synuclein - metabolism; Animals; Brain - metabolism; Brain - pathology; Female; Humans; Male; Mice; Middle Aged; Parkinson Disease - diagnosis; Parkinson Disease - genetics; Parkinson Disease - metabolism; Parkinson's disease; Quiescent SAA; Seed amplification assay; Seeding activity; Sensitivity and Specificity; Skin - metabolism; Skin - pathology; α-Synuclein; α-Synuclein; Parkinson's disease; Seeding activity; Quiescent SAA; Seed amplification assay

Seed amplification assays (SAA) enable the amplification of pathological misfolded proteins, including α-synuclein (αSyn), in both tissue homogenates and body fluids of Parkinson's disease (PD) patients. SAA involves repeated cycles of shaking or sonication coupled with incubation periods. Howe...