Release of EPA and DHA from salmon oil – a comparison of in vitro digestion with human and porcine gastrointestinal enzymes

• Published in: British journal of nutrition Vol. 110; no. 8; pp. 1402 - 1410
• Main Authors: Aarak, K. E., Kirkhus, B., Holm, H., Vogt, G., Jacobsen, M., Vegarud, G. E.
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 28.10.2013
• Subjects: Animals; bile; Bile - enzymology; Bile Acids and Salts - metabolism; bile salts; Biological and medical sciences; Body Fluids - enzymology; Cattle; Comparative studies; Digestion; Digestive system; Docosahexaenoic Acids - analysis; duodenal juices; Duodenum - metabolism; Eicosapentaenoic Acid - analysis; Enzymes; Fatty acids; Feeding. Feeding behavior; Fish oils; Fish Oils - chemistry; Fundamental and applied biological sciences. Psychology; gastrointestinal system; Gastrointestinal tract; Hogs; Human subjects; Humans; in vitro digestion; ionization; Models, Biological; Molecular Nutrition; pancreatin; Pancreatin - metabolism; polyunsaturated fatty acids; Salmon; salmon oil; Salmonidae; Sheep; solid phase extraction; Swine; Time Factors; Vertebrates: anatomy and physiology, studies on body, several organs or systems; n-3 PUFA; EPA; In vitro lipid digestion; Human and porcine gastrointestinal enzymes; DHA; Salmon oil; Lipolysis; Lipids; Gastrointestinal; n-3 fatty acid; Ungulata; Release; Human; Nutrition; Digestive system; Enzyme; Polyunsaturated fatty acid; Digestion; In vitro; Pig; Vertebrata; Mammalia; Animal; Unsaturated fatty acid; Artiodactyla; Comparative study

In the present study, we hypothesised whether in vitro digestion of salmon oil would release different amounts of PUFA depending on the origin of the lipolytic enzymes used. For this purpose, in vitro digestion of salmon oil (SO) was performed using human duodenal juice (HDJ) or a commercial enzyme...