Safety assessment of Enterococcus lactis based on comparative genomics and phenotypic analysis
is sometimes used in food production; however, its acquisition of antibiotic resistance has become an alarming health concern. The species is closely related to and has good probiotic potential. This study aimed to investigate the antibiotic resistance of We analyzed the antibiotic resistance phenot...
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| Published in | Frontiers in microbiology Vol. 14; p. 1196558 |
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| Main Authors | , , , , , , , |
| Format | Journal Article |
| Language | English |
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22.05.2023
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| Abstract | is sometimes used in food production; however, its acquisition of antibiotic resistance has become an alarming health concern. The
species is closely related to
and has good probiotic potential. This study aimed to investigate the antibiotic resistance of
We analyzed the antibiotic resistance phenotype and whole-genome sequences of 60
isolates (23, 29, and 8 isolates from dairy products, Rice wine Koji, and human feces, respectively). These isolates showed varying degree of resistance to 13 antibiotics, and were sensitive to ampicillin and linezolid. The
genomes carried only a subset of commonly reported antibiotic resistance genes (ARGs) in
. Five ARGs were detected across the investigated
, including two universally present genes (
and
) and three rarely detected ARGs (
,
, and
). To identify other undescribed antibiotic resistance-encoding genes, a genome-wide association study was performed, returning 160 potential resistance genes that were associated with six antibiotics, namely chloramphenicol, vancomycin, clindamycin, erythromycin, quinupristin-dalfopristin, and rifampicin. Only around one-third of these genes encode known biological functions, including cellular metabolism, membrane transport, and DNA synthesis. This work identified interesting targets for future study of antibiotic resistance in
. The fact that the lower number of ARGs present in
supports that it may be an alternative to
for use in the food industry. Data generated in this work is of interest to the dairy industry. |
|---|---|
| AbstractList | Enterococcus faecium is sometimes used in food production; however, its acquisition of antibiotic resistance has become an alarming health concern. The E. lactis species is closely related to E. faecium and has good probiotic potential. This study aimed to investigate the antibiotic resistance of E. lactis. We analyzed the antibiotic resistance phenotype and whole-genome sequences of 60 E. lactis isolates (23, 29, and 8 isolates from dairy products, Rice wine Koji, and human feces, respectively). These isolates showed varying degree of resistance to 13 antibiotics, and were sensitive to ampicillin and linezolid. The E. lactis genomes carried only a subset of commonly reported antibiotic resistance genes (ARGs) in E. faecium. Five ARGs were detected across the investigated E. lactis, including two universally present genes (msrC and AAC(6′)-Ii) and three rarely detected ARGs (tet(L), tetM, and efmA). To identify other undescribed antibiotic resistance-encoding genes, a genome-wide association study was performed, returning 160 potential resistance genes that were associated with six antibiotics, namely chloramphenicol, vancomycin, clindamycin, erythromycin, quinupristin-dalfopristin, and rifampicin. Only around one-third of these genes encode known biological functions, including cellular metabolism, membrane transport, and DNA synthesis. This work identified interesting targets for future study of antibiotic resistance in E. lactis. The fact that the lower number of ARGs present in E. lactis supports that it may be an alternative to E. faecalis for use in the food industry. Data generated in this work is of interest to the dairy industry. Enterococcus faecium is sometimes used in food production; however, its acquisition of antibiotic resistance has become an alarming health concern. The E. lactis species is closely related to E. faecium and has good probiotic potential. This study aimed to investigate the antibiotic resistance of E. lactis. We analyzed the antibiotic resistance phenotype and whole-genome sequences of 60 E. lactis isolates (23, 29, and 8 isolates from dairy products, Rice wine Koji, and human feces, respectively). These isolates showed varying degree of resistance to 13 antibiotics, and were sensitive to ampicillin and linezolid. The E. lactis genomes carried only a subset of commonly reported antibiotic resistance genes (ARGs) in E. faecium . Five ARGs were detected across the investigated E. lactis , including two universally present genes ( msrC and AAC(6′)-Ii ) and three rarely detected ARGs ( tet(L) , tetM , and efmA ). To identify other undescribed antibiotic resistance-encoding genes, a genome-wide association study was performed, returning 160 potential resistance genes that were associated with six antibiotics, namely chloramphenicol, vancomycin, clindamycin, erythromycin, quinupristin-dalfopristin, and rifampicin. Only around one-third of these genes encode known biological functions, including cellular metabolism, membrane transport, and DNA synthesis. This work identified interesting targets for future study of antibiotic resistance in E. lactis . The fact that the lower number of ARGs present in E. lactis supports that it may be an alternative to E. faecalis for use in the food industry. Data generated in this work is of interest to the dairy industry. is sometimes used in food production; however, its acquisition of antibiotic resistance has become an alarming health concern. The species is closely related to and has good probiotic potential. This study aimed to investigate the antibiotic resistance of We analyzed the antibiotic resistance phenotype and whole-genome sequences of 60 isolates (23, 29, and 8 isolates from dairy products, Rice wine Koji, and human feces, respectively). These isolates showed varying degree of resistance to 13 antibiotics, and were sensitive to ampicillin and linezolid. The genomes carried only a subset of commonly reported antibiotic resistance genes (ARGs) in . Five ARGs were detected across the investigated , including two universally present genes ( and ) and three rarely detected ARGs ( , , and ). To identify other undescribed antibiotic resistance-encoding genes, a genome-wide association study was performed, returning 160 potential resistance genes that were associated with six antibiotics, namely chloramphenicol, vancomycin, clindamycin, erythromycin, quinupristin-dalfopristin, and rifampicin. Only around one-third of these genes encode known biological functions, including cellular metabolism, membrane transport, and DNA synthesis. This work identified interesting targets for future study of antibiotic resistance in . The fact that the lower number of ARGs present in supports that it may be an alternative to for use in the food industry. Data generated in this work is of interest to the dairy industry. |
| Author | Ma, Xinwei Awad, Sameh Zhong, Zhi Lu, Jingda Du, Muying Xu, Sheng Zhang, Yixin Shen, Tingting |
| AuthorAffiliation | 6 Chinese-Hungarian Cooperative Research Centre for Food Science, Southwest University , Chongqing , China 2 Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University , Hohhot , China 5 College of Food Science, Southwest University , Chongqing , China 3 Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering , Hohhot , China 1 Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University , Hohhot , China 4 Department of Dairy Science and Technology, Faculty of Agriculture, Alexandria University , Alexandria , Egypt |
| AuthorAffiliation_xml | – name: 2 Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University , Hohhot , China – name: 4 Department of Dairy Science and Technology, Faculty of Agriculture, Alexandria University , Alexandria , Egypt – name: 3 Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering , Hohhot , China – name: 6 Chinese-Hungarian Cooperative Research Centre for Food Science, Southwest University , Chongqing , China – name: 5 College of Food Science, Southwest University , Chongqing , China – name: 1 Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University , Hohhot , China |
| Author_xml | – sequence: 1 givenname: Jingda surname: Lu fullname: Lu, Jingda organization: Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Hohhot, China – sequence: 2 givenname: Tingting surname: Shen fullname: Shen, Tingting organization: Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Hohhot, China – sequence: 3 givenname: Yixin surname: Zhang fullname: Zhang, Yixin organization: Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Hohhot, China – sequence: 4 givenname: Xinwei surname: Ma fullname: Ma, Xinwei organization: Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Hohhot, China – sequence: 5 givenname: Sheng surname: Xu fullname: Xu, Sheng organization: Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Hohhot, China – sequence: 6 givenname: Sameh surname: Awad fullname: Awad, Sameh organization: Department of Dairy Science and Technology, Faculty of Agriculture, Alexandria University, Alexandria, Egypt – sequence: 7 givenname: Muying surname: Du fullname: Du, Muying organization: Chinese-Hungarian Cooperative Research Centre for Food Science, Southwest University, Chongqing, China – sequence: 8 givenname: Zhi surname: Zhong fullname: Zhong, Zhi organization: Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Hohhot, China |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/37283930$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1038/nrmicro2761 10.1128/AAC.44.9.2585-2587.2000 10.1128/CMR.00058-18 10.3390/foods11071046 10.1093/bioinformatics/btu153 10.1590/0037-8682-0353-2021 10.5851/kosfa.2016.36.3.352 10.1186/2047-217X-1-18 10.5851/kosfa.2017.37.5.670 10.1186/s12864-019-5975-8 10.1128/AAC.47.12.3675-3681.2003 10.1016/j.ijfoodmicro.2004.12.025 10.1128/AAC.37.9.1896 10.1016/S0168-1605(99)00007-0 10.1128/mBio.03284-19 10.1016/S0140-6736(01)13797-9 10.1093/nar/gkz239 10.1007/978-1-4615-2668-1_3 10.1016/j.molp.2020.06.009 10.1016/s0168-1605(03)00190-9 10.1016/S0021-9258(20)89626-4 10.1186/s13059-016-1108-8 10.1099/ijs.0.030825-0 10.1128/microbiolspec.VMBF-0016-2015 10.1128/JB.00421-06 10.1186/s12866-019-1683-4 10.1007/s13594-011-0016-7 10.1186/s12864-019-5588-2 10.1099/ijs.0.64483-0 10.4103/ijmm.IJMM_19_355 10.1093/jac/dkaa041 10.1073/pnas.1113051109 10.1016/j.fm.2011.12.002 10.1099/00207713-34-1-31 10.1101/gr.073585.107 10.1021/bi034148h 10.1111/j.1471-0307.2004.00122.x 10.1128/AEM.67.4.1628-1635.2001 10.1093/nar/gkx343 10.1007/s11021-005-0127-3 10.1093/bioinformatics/btv421 10.1016/j.mib.2017.11.030 10.1016/j.ijfoodmicro.2011.08.014 |
| ContentType | Journal Article |
| Copyright | Copyright © 2023 Lu, Shen, Zhang, Ma, Xu, Awad, Du and Zhong. Copyright © 2023 Lu, Shen, Zhang, Ma, Xu, Awad, Du and Zhong. 2023 Lu, Shen, Zhang, Ma, Xu, Awad, Du and Zhong |
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| Keywords | safety assessment comparative genomics analysis antibiotic resistance genome-wide association study Enterococcus lactis |
| Language | English |
| License | Copyright © 2023 Lu, Shen, Zhang, Ma, Xu, Awad, Du and Zhong. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
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| References | (ref18) 2012; 10 Zhong (ref47) 2019; 20 Andrewes (ref1) 1906; 168 Cebrián (ref6) 2012; 30 Neela (ref35) 2007; 45 Arredondo-Alonso (ref3) 2020; 11 Çitak (ref8) 2004; 57 Eisenberger (ref19) 2020; 75 Costa (ref11) 1993; 37 Vasconcellos (ref45) 2022; 55 Luo (ref30) 2012; 1 Kürekci (ref26) 2016; 36 Munita (ref34) 2016; 4 Sukhodolets (ref41) 2005; 74 Vilella (ref46) 2009; 19 Gao (ref21) 2018; 41 Brynildsrud (ref5) 2016; 17 Demirgül (ref15) 2017; 37 Seemann (ref40) 2014; 30 Page (ref36) 2015; 31 Draker (ref16) 2003; 42 Kim (ref24) 2022; 11 Lopes (ref29) 2005; 103 Bertelli (ref4) 2017; 45 Goris (ref23) 2007; 57 Klare (ref25) 2003; 88 García-Solache (ref22) 2019; 32 Schleifer (ref39) 1984; 34 Morandi (ref32) 2012; 62 Das (ref13) 2020; 38 Lepage (ref27) 2006; 188 Tang (ref42) 2019; 20 Connell (ref10) 2003; 47 van Rooijen (ref44) 1991; 266 Eaton (ref17) 2001; 67 Tholander (ref43) 2012; 109 Letunic (ref28) 2019; 47 Moreno (ref33) 2006; 106 Sanderson (ref37) 2020; 20 Arias (ref2) 2012; 10 Santagati (ref38) 2000; 44 Cockerill (ref9) 2012 Franz (ref20) 1999; 47 Chen (ref7) 2020; 13 De Vuyst (ref14) 1994 Morandi (ref31) 2011; 91 |
| References_xml | – volume: 10 start-page: 266 year: 2012 ident: ref2 article-title: The rise of the enterococcus: beyond vancomycin resistance publication-title: Nat. Rev. Microbiol. doi: 10.1038/nrmicro2761 contributor: fullname: Arias – volume: 44 start-page: 2585 year: 2000 ident: ref38 article-title: Characterization of a genetic element carrying the macrolide efflux gene mef (a) in Streptococcus pneumoniae publication-title: Antimicrob. Agents Chemother. doi: 10.1128/AAC.44.9.2585-2587.2000 contributor: fullname: Santagati – volume: 32 start-page: e00058 year: 2019 ident: ref22 article-title: The Enterococcus: a model of adaptability to its environment publication-title: Clin. Microbiol. Rev. doi: 10.1128/CMR.00058-18 contributor: fullname: García-Solache – volume: 11 start-page: 1046 year: 2022 ident: ref24 article-title: Differentiating between Enterococcus faecium and Enterococcus lactis by matrix-assisted laser desorption ionization time-of-flight mass spectrometry publication-title: Foods doi: 10.3390/foods11071046 contributor: fullname: Kim – volume: 30 start-page: 2068 year: 2014 ident: ref40 article-title: Prokka: rapid prokaryotic genome annotation publication-title: Bioinformatics doi: 10.1093/bioinformatics/btu153 contributor: fullname: Seemann – volume: 55 start-page: e0353 year: 2022 ident: ref45 article-title: Staphylococcus aureus and Enterococcus faecium isolated from pigeon droppings (Columba livia) in the external environment close to hospitals publication-title: Rev. Soc. Bras. Med. Trop. doi: 10.1590/0037-8682-0353-2021 contributor: fullname: Vasconcellos – volume: 10 start-page: 2740 year: 2012 ident: ref18 article-title: Guidance on the assessment of bacterial susceptibility to antimicrobials of human and veterinary importance publication-title: EFSA J. – volume: 36 start-page: 352 year: 2016 ident: ref26 article-title: Characterisation of phenotypic and genotypic antibiotic resistance profile of enterococci from cheeses in Turkey publication-title: Korean J. Food Sci. Anim. Resour. doi: 10.5851/kosfa.2016.36.3.352 contributor: fullname: Kürekci – volume: 1 start-page: 18 year: 2012 ident: ref30 article-title: SOAPdenovo2: an empirically improved memory-efficient short-read de novo assembler publication-title: Gigascience doi: 10.1186/2047-217X-1-18 contributor: fullname: Luo – volume: 37 start-page: 670 year: 2017 ident: ref15 article-title: Detection of antibiotic resistance and resistance genes in enterococci isolated from sucuk, a traditional Turkish dry-fermented sausage publication-title: Korean J. Food Sci. Anim. Resour. doi: 10.5851/kosfa.2017.37.5.670 contributor: fullname: Demirgül – volume: 20 start-page: 602 year: 2019 ident: ref47 article-title: Comparative genomic analysis revealed great plasticity and environmental adaptation of the genomes of Enterococcus faecium publication-title: BMC Genomics doi: 10.1186/s12864-019-5975-8 contributor: fullname: Zhong – volume: 47 start-page: 3675 year: 2003 ident: ref10 article-title: Ribosomal protection proteins and their mechanism of tetracycline resistance publication-title: Antimicrob. Agents Chemother. doi: 10.1128/AAC.47.12.3675-3681.2003 contributor: fullname: Connell – volume: 103 start-page: 191 year: 2005 ident: ref29 article-title: Antimicrobial resistance profiles of dairy and clinical isolates and type strains of enterococci publication-title: Int. J. Food Microbiol. doi: 10.1016/j.ijfoodmicro.2004.12.025 contributor: fullname: Lopes – volume: 37 start-page: 1896 year: 1993 ident: ref11 article-title: Characterization of the chromosomal aac (6′)-ii gene specific for Enterococcus faecium publication-title: Antimicrob. Agents Chemother. doi: 10.1128/AAC.37.9.1896 contributor: fullname: Costa – volume: 47 start-page: 1 year: 1999 ident: ref20 article-title: Enterococci at the crossroads of food safety? publication-title: Int. J. Food Microbiol. doi: 10.1016/S0168-1605(99)00007-0 contributor: fullname: Franz – volume: 11 start-page: e03219 year: 2020 ident: ref3 article-title: Plasmids shaped the recent emergence of the major nosocomial pathogen Enterococcus faecium publication-title: MBio doi: 10.1128/mBio.03284-19 contributor: fullname: Arredondo-Alonso – volume: 168 start-page: 775 year: 1906 ident: ref1 article-title: A study of the streptococci pathogenic for man publication-title: Lancet doi: 10.1016/S0140-6736(01)13797-9 contributor: fullname: Andrewes – volume: 47 start-page: W256 year: 2019 ident: ref28 article-title: Interactive tree of life (iTOL) v4: recent updates and new developments publication-title: Nucleic Acids Res. doi: 10.1093/nar/gkz239 contributor: fullname: Letunic – start-page: 91 volume-title: Bacteriocins of lactic acid bacteria: microbiology, genetics and applications year: 1994 ident: ref14 article-title: Antimicrobial potential of lactic acid bacteria doi: 10.1007/978-1-4615-2668-1_3 contributor: fullname: De Vuyst – volume: 45 start-page: 64 year: 2007 ident: ref35 article-title: The diversity of multi-drug resistance profiles in tetracycline-resistant Vibrio species isolated from coastal sediments and seawater publication-title: J. Microbiol. contributor: fullname: Neela – volume: 13 start-page: 1194 year: 2020 ident: ref7 article-title: TBtools: an integrative toolkit developed for interactive analyses of big biological data publication-title: Mol. Plant doi: 10.1016/j.molp.2020.06.009 contributor: fullname: Chen – volume: 88 start-page: 269 year: 2003 ident: ref25 article-title: Occurrence and spread of antibiotic resistances in Enterococcus faecium publication-title: Int. J. Food Microbiol. doi: 10.1016/s0168-1605(03)00190-9 contributor: fullname: Klare – volume: 266 start-page: 7176 year: 1991 ident: ref44 article-title: Molecular cloning, characterization, and nucleotide sequence of the tagatose 6-phosphate pathway gene cluster of the lactose operon of Lactococcus lactis publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(20)89626-4 contributor: fullname: van Rooijen – volume: 17 start-page: 238 year: 2016 ident: ref5 article-title: Rapid scoring of genes in microbial pan-genome-wide association studies with Scoary publication-title: Genome Biol. doi: 10.1186/s13059-016-1108-8 contributor: fullname: Brynildsrud – volume: 62 start-page: 1992 year: 2012 ident: ref32 article-title: Enterococcus lactis sp. nov., from Italian raw milk cheeses publication-title: Int. J. Syst. Evol. Microbiol. doi: 10.1099/ijs.0.030825-0 contributor: fullname: Morandi – volume: 4 start-page: 481 year: 2016 ident: ref34 article-title: Mechanisms of antibiotic resistance publication-title: Microbiol. Spectr. doi: 10.1128/microbiolspec.VMBF-0016-2015 contributor: fullname: Munita – volume-title: Performance standards for antimicrobial disk susceptibility testing: Approved standard year: 2012 ident: ref9 contributor: fullname: Cockerill – volume: 188 start-page: 6858 year: 2006 ident: ref27 article-title: Comparative genomic hybridization analysis of Enterococcus faecalis: identification of genes absent from food strains publication-title: J. Bacteriol. doi: 10.1128/JB.00421-06 contributor: fullname: Lepage – volume: 20 start-page: 1 year: 2020 ident: ref37 article-title: Comparative genomics of multidrug-resistant Enterococcus spp. isolated from wastewater treatment plants publication-title: BMC Microbiol. doi: 10.1186/s12866-019-1683-4 contributor: fullname: Sanderson – volume: 91 start-page: 341 year: 2011 ident: ref31 article-title: Technological, phenotypic and genotypic characterisation of wild lactic acid bacteria involved in the production of Bitto PDO Italian cheese publication-title: Dairy Sci. Technol. doi: 10.1007/s13594-011-0016-7 contributor: fullname: Morandi – volume: 20 start-page: 212 year: 2019 ident: ref42 article-title: Comparative genomic analysis of 127 Escherichia coli strains isolated from domestic animals with diarrhea in China publication-title: BMC Genomics doi: 10.1186/s12864-019-5588-2 contributor: fullname: Tang – volume: 57 start-page: 81 year: 2007 ident: ref23 article-title: DNA–DNA hybridization values and their relationship to whole-genome sequence similarities publication-title: Int. J. Syst. Evol. Microbiol. doi: 10.1099/ijs.0.64483-0 contributor: fullname: Goris – volume: 38 start-page: 200 year: 2020 ident: ref13 article-title: Biofilm synthesis and other virulence factors in multidrug-resistant uropathogenic enterococci isolated in northern India publication-title: Indian J. Med. Microbiol. doi: 10.4103/ijmm.IJMM_19_355 contributor: fullname: Das – volume: 75 start-page: 1398 year: 2020 ident: ref19 article-title: Whole-genome analysis of vancomycin-resistant Enterococcus faecium causing nosocomial outbreaks suggests the occurrence of few endemic clonal lineages in Bavaria, Germany publication-title: J. Antimicrob. Chemother. doi: 10.1093/jac/dkaa041 contributor: fullname: Eisenberger – volume: 109 start-page: 9798 year: 2012 ident: ref43 article-title: Discovery of antimicrobial ribonucleotide reductase inhibitors by screening in microwell format publication-title: Proc. Natl. Acad. Sci. doi: 10.1073/pnas.1113051109 contributor: fullname: Tholander – volume: 30 start-page: 59 year: 2012 ident: ref6 article-title: Characterization of functional, safety, and probiotic properties of Enterococcus faecalis UGRA10, a new AS-48-producer strain publication-title: Food Microbiol. doi: 10.1016/j.fm.2011.12.002 contributor: fullname: Cebrián – volume: 34 start-page: 31 year: 1984 ident: ref39 article-title: Transfer of Streptococcus faecalis and Streptococcus faecium to the genus Enterococcus nom. Rev. as Enterococcus faecalis comb. nov. and Enterococcus faecium comb. nov publication-title: Int. J. Syst. Bacteriol. doi: 10.1099/00207713-34-1-31 contributor: fullname: Schleifer – volume: 19 start-page: 327 year: 2009 ident: ref46 article-title: EnsemblCompara GeneTrees: complete, duplication-aware phylogenetic trees in vertebrates publication-title: Genome Res. doi: 10.1101/gr.073585.107 contributor: fullname: Vilella – volume: 42 start-page: 6565 year: 2003 ident: ref16 article-title: Kinetic mechanism of the GCN5-related chromosomal aminoglycoside acetyltransferase AAC (6 ‘)-ii from Enterococcus faecium: evidence of dimer subunit cooperativity publication-title: Biochemistry doi: 10.1021/bi034148h contributor: fullname: Draker – volume: 57 start-page: 27 year: 2004 ident: ref8 article-title: Antibiotic resistance and incidence of Enterococcus species in Turkish white cheese publication-title: Int. J. Dairy Technol. doi: 10.1111/j.1471-0307.2004.00122.x contributor: fullname: Çitak – volume: 67 start-page: 1628 year: 2001 ident: ref17 article-title: Molecular screening of Enterococcus virulence determinants and potential for genetic exchange between food and medical isolates publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.67.4.1628-1635.2001 contributor: fullname: Eaton – volume: 45 start-page: W30 year: 2017 ident: ref4 article-title: IslandViewer 4: expanded prediction of genomic islands for larger-scale datasets publication-title: Nucl. Acids Res. doi: 10.1093/nar/gkx343 contributor: fullname: Bertelli – volume: 74 start-page: 704 year: 2005 ident: ref41 article-title: The lactic acid enterococci Enterococcus faecium and Enterococcus durans: nucleotide sequence diversity in 16S rRNA genes publication-title: Microbiology doi: 10.1007/s11021-005-0127-3 contributor: fullname: Sukhodolets – volume: 31 start-page: 3691 year: 2015 ident: ref36 article-title: Roary: rapid large-scale prokaryote pan genome analysis publication-title: Bioinformatics doi: 10.1093/bioinformatics/btv421 contributor: fullname: Page – volume: 41 start-page: 76 year: 2018 ident: ref21 article-title: Evolution of virulence in Enterococcus faecium, a hospital-adapted opportunistic pathogen publication-title: Curr. Opin. Microbiol. doi: 10.1016/j.mib.2017.11.030 contributor: fullname: Gao – volume: 106 start-page: 1 year: 2006 ident: ref33 article-title: The role and application of enterococci in food and health publication-title: Int. J. Food Microbiol. doi: 10.1016/j.ijfoodmicro.2011.08.014 contributor: fullname: Moreno |
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| Snippet | is sometimes used in food production; however, its acquisition of antibiotic resistance has become an alarming health concern. The
species is closely related... Enterococcus faecium is sometimes used in food production; however, its acquisition of antibiotic resistance has become an alarming health concern. The E.... Enterococcus faecium is sometimes used in food production; however, its acquisition of antibiotic resistance has become an alarming health concern. The E.... |
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| SubjectTerms | antibiotic resistance comparative genomics analysis Enterococcus lactis genome-wide association study Microbiology safety assessment |
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| Title | Safety assessment of Enterococcus lactis based on comparative genomics and phenotypic analysis |
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