Label-Free QCM Immunosensor for the Detection of Ochratoxin A
Ochratoxin A (OTA) is a potent mycotoxin that poses a risk in food and feed moieties and subject to worldwide regulation. Laboratory-based analytical methods are traditionally employed for reliable OTA quantification, but these methods cannot provide rapid and on-site analysis, where biosensors fill...
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| Published in | Sensors (Basel, Switzerland) Vol. 18; no. 4; p. 1161 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
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Switzerland
MDPI AG
11.04.2018
MDPI |
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| Online Access | Get full text |
| ISSN | 1424-8220 1424-8220 |
| DOI | 10.3390/s18041161 |
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| Abstract | Ochratoxin A (OTA) is a potent mycotoxin that poses a risk in food and feed moieties and subject to worldwide regulation. Laboratory-based analytical methods are traditionally employed for reliable OTA quantification, but these methods cannot provide rapid and on-site analysis, where biosensors fill this gap. In this study a label-free quartz crystal microbalance (QCM)-based immunosensor for the detection of OTA, which is one of the most important small molecule contaminants, was developed by direct immobilization of OTA to amine-bearing sensor surfaces using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) chemistry. The protein-free sensor surface enabled regeneration of sensor surface with 50 mM NaOH and 1% SDS up to 13 times without loss of performance, which would disrupt a protein-containing sensor surface. We developed a QCM immunosensor using the developed sensor surface with a 17.2–200 ng/mL detection range which can be used for on-site detection of feedstuffs. |
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| AbstractList | Ochratoxin A (OTA) is a potent mycotoxin that poses a risk in food and feed moieties and subject to worldwide regulation. Laboratory-based analytical methods are traditionally employed for reliable OTA quantification, but these methods cannot provide rapid and on-site analysis, where biosensors fill this gap. In this study a label-free quartz crystal microbalance (QCM)-based immunosensor for the detection of OTA, which is one of the most important small molecule contaminants, was developed by direct immobilization of OTA to amine-bearing sensor surfaces using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) chemistry. The protein-free sensor surface enabled regeneration of sensor surface with 50 mM NaOH and 1% SDS up to 13 times without loss of performance, which would disrupt a protein-containing sensor surface. We developed a QCM immunosensor using the developed sensor surface with a 17.2–200 ng/mL detection range which can be used for on-site detection of feedstuffs. Ochratoxin A (OTA) is a potent mycotoxin that poses a risk in food and feed moieties and subject to worldwide regulation. Laboratory-based analytical methods are traditionally employed for reliable OTA quantification, but these methods cannot provide rapid and on-site analysis, where biosensors fill this gap. In this study a label-free quartz crystal microbalance (QCM)-based immunosensor for the detection of OTA, which is one of the most important small molecule contaminants, was developed by direct immobilization of OTA to amine-bearing sensor surfaces using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) chemistry. The protein-free sensor surface enabled regeneration of sensor surface with 50 mM NaOH and 1% SDS up to 13 times without loss of performance, which would disrupt a protein-containing sensor surface. We developed a QCM immunosensor using the developed sensor surface with a 17.2-200 ng/mL detection range which can be used for on-site detection of feedstuffs.Ochratoxin A (OTA) is a potent mycotoxin that poses a risk in food and feed moieties and subject to worldwide regulation. Laboratory-based analytical methods are traditionally employed for reliable OTA quantification, but these methods cannot provide rapid and on-site analysis, where biosensors fill this gap. In this study a label-free quartz crystal microbalance (QCM)-based immunosensor for the detection of OTA, which is one of the most important small molecule contaminants, was developed by direct immobilization of OTA to amine-bearing sensor surfaces using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) chemistry. The protein-free sensor surface enabled regeneration of sensor surface with 50 mM NaOH and 1% SDS up to 13 times without loss of performance, which would disrupt a protein-containing sensor surface. We developed a QCM immunosensor using the developed sensor surface with a 17.2-200 ng/mL detection range which can be used for on-site detection of feedstuffs. |
| Author | Öztürk, Zafer Laguna, Duygu Özen, Fehime Ertekin, Özlem Öztürk, Selma Pirinçci, Şerife |
| AuthorAffiliation | 2 Department of Medical Genetics and Molecular Biology, Kocaeli University, Umuttepe, Kocaeli 41100, Turkey 1 TÜBİTAK, The Scientific and Technological Research Council of Turkey, Marmara Research Center, Genetic Engineering and Biotechnology Institute, Gebze, Kocaeli 41400, Turkey; duygu.ercan@windowslive.com (D.E.L.); f.seymaozen@gmail.com (F.Ş.Ö.); selma.ozturk@tubitak.gov.tr (S.Ö.) 3 Department of Physics, Gebze Technical University, Gebze, Kocaeli 41400, Turkey; zozturk@gtu.edu.tr |
| AuthorAffiliation_xml | – name: 1 TÜBİTAK, The Scientific and Technological Research Council of Turkey, Marmara Research Center, Genetic Engineering and Biotechnology Institute, Gebze, Kocaeli 41400, Turkey; duygu.ercan@windowslive.com (D.E.L.); f.seymaozen@gmail.com (F.Ş.Ö.); selma.ozturk@tubitak.gov.tr (S.Ö.) – name: 3 Department of Physics, Gebze Technical University, Gebze, Kocaeli 41400, Turkey; zozturk@gtu.edu.tr – name: 2 Department of Medical Genetics and Molecular Biology, Kocaeli University, Umuttepe, Kocaeli 41100, Turkey |
| Author_xml | – sequence: 1 givenname: Şerife orcidid: 0000-0001-5659-5806 surname: Pirinçci fullname: Pirinçci, Şerife – sequence: 2 givenname: Özlem surname: Ertekin fullname: Ertekin, Özlem – sequence: 3 givenname: Duygu surname: Laguna fullname: Laguna, Duygu – sequence: 4 givenname: Fehime surname: Özen fullname: Özen, Fehime – sequence: 5 givenname: Zafer surname: Öztürk fullname: Öztürk, Zafer – sequence: 6 givenname: Selma surname: Öztürk fullname: Öztürk, Selma |
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| Keywords | immobilization immunobiosensor regeneration quartz crystal microbalance QCM OTA Ochratoxin A |
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