Virus Infection Variability by Single-Cell Profiling

• Published in: Viruses Vol. 13; no. 8; p. 1568
• Main Authors: Suomalainen, Maarit, Greber, Urs F
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 09.08.2021; MDPI
• Subjects: Adenoviruses; Animals; cell state; cell-to-cell variability; Chromatin; Click Chemistry - methods; Dengue fever; Flow cytometry; Fluorescence in situ hybridization; Gene expression; Genome, Viral; Genomes; Herpes simplex; Humans; Hybridization; Immune response; Immunity, Innate; Immunofluorescence; In Situ Hybridization, Fluorescence - methods; Infections; Influenza; Innate immunity; Interferon; Localization; Mice; Microscopy; non-genetic variability; Plaques; Proteins; Review; RNAseq; Single-Cell Analysis; single-cell infection; Transcription; Virion - genetics; Virion - metabolism; Virions; Virus Diseases - metabolism; Virus Diseases - virology; virus imaging; Virus Physiological Phenomena; Virus Replication; Viruses; Viruses - classification; Viruses - genetics; Viruses - metabolism; replication; single transcript fluorescence in situ hybridization; transcription; non-genetic variability; click chemistry; RNAseq; egress; single-cell infection; virus imaging; assembly; virus entry; cell-to-cell variability; cell state; persistence and lysis

Cell-to-cell variability of infection has long been known, yet it has remained one of the least understood phenomena in infection research. It impacts on disease onset and development, yet only recently underlying mechanisms have been studied in clonal cell cultures by single-virion immunofluorescen...