Using brain organoids to understand Zika virus-induced microcephaly
Technologies to differentiate human pluripotent stem cells into three-dimensional organized structures that resemble in vivo organs are pushing the frontiers of human disease modeling and drug development. In response to the global health emergency posed by the Zika virus (ZIKV) outbreak, brain orga...
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| Published in | Development (Cambridge) Vol. 144; no. 6; pp. 952 - 957 |
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| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
The Company of Biologists Ltd
15.03.2017
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| Subjects | |
| Online Access | Get full text |
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| Abstract | Technologies to differentiate human pluripotent stem cells into three-dimensional organized structures that resemble in vivo organs are pushing the frontiers of human disease modeling and drug development. In response to the global health emergency posed by the Zika virus (ZIKV) outbreak, brain organoids engineered to mimic the developing human fetal brain have been employed to model ZIKV-induced microcephaly. Here, we discuss the advantages of brain organoids over other model systems to study development and highlight recent advances in understanding ZIKV pathophysiology and its underlying pathogenesis mechanisms. We further discuss perspectives on overcoming limitations of current organoid systems for their future use in ZIKV research. |
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| AbstractList | Technologies to differentiate human pluripotent stem cells into three-dimensional organized structures that resemble in vivo organs are pushing the frontiers of human disease modeling and drug development. In response to the global health emergency posed by the Zika virus (ZIKV) outbreak, brain organoids engineered to mimic the developing human fetal brain have been employed to model ZIKV-induced microcephaly. Here, we discuss the advantages of brain organoids over other model systems to study development and highlight recent advances in understanding ZIKV pathophysiology and its underlying pathogenesis mechanisms. We further discuss perspectives on overcoming limitations of current organoid systems for their future use in ZIKV research.Technologies to differentiate human pluripotent stem cells into three-dimensional organized structures that resemble in vivo organs are pushing the frontiers of human disease modeling and drug development. In response to the global health emergency posed by the Zika virus (ZIKV) outbreak, brain organoids engineered to mimic the developing human fetal brain have been employed to model ZIKV-induced microcephaly. Here, we discuss the advantages of brain organoids over other model systems to study development and highlight recent advances in understanding ZIKV pathophysiology and its underlying pathogenesis mechanisms. We further discuss perspectives on overcoming limitations of current organoid systems for their future use in ZIKV research. Technologies to differentiate human pluripotent stem cells into three-dimensional organized structures that resemble in vivo organs are pushing the frontiers of human disease modeling and drug development. In response to the global health emergency posed by the Zika virus (ZIKV) outbreak, brain organoids engineered to mimic the developing human fetal brain have been employed to model ZIKV-induced microcephaly. Here, we discuss the advantages of brain organoids over other model systems to study development and highlight recent advances in understanding ZIKV pathophysiology and its underlying pathogenesis mechanisms. We further discuss perspectives on overcoming limitations of current organoid systems for their future use in ZIKV research. Technologies to differentiate human pluripotent stem cells into three-dimensional organized structures that resemble in vivo organs are pushing the frontiers of human disease modeling and drug development. In response to the global health emergency posed by the Zika virus (ZIKV) outbreak, brain organoids engineered to mimic the developing human fetal brain have been employed to model ZIKV-induced microcephaly. Here, we discuss the advantages of brain organoids over other model systems to study development and highlight recent advances in understanding ZIKV pathophysiology and its underlying pathogenesis mechanisms. We further discuss perspectives on overcoming limitations of current organoid systems for their future use in ZIKV research. Summary: This Spotlight article summarises the latest advances in using cerebral organoids to model Zika virus infection and the resulting pathology. Technologies to differentiate human pluripotent stem cells into three-dimensional organized structures that resemble in vivo organs are pushing the frontiers of human disease modeling and drug development. In response to the global health emergency posed by the Zika virus (ZIKV) outbreak, brain organoids engineered to mimic the developing human fetal brain have been employed to model ZIKV-induced microcephaly. Here, we discuss the advantages of brain organoids over other model systems to study development and highlight recent advances in understanding ZIKV pathophysiology and its underlying pathogenesis mechanisms. We further discuss perspectives on overcoming limitations of current organoid systems for their future use in ZIKV research. Summary: This Spotlight article summarises the latest advances in using cerebral organoids to model Zika virus infection and the resulting pathology. Technologies to differentiate human pluripotent stem cells into three-dimensional organized structures that resemble organs are pushing the frontiers of human disease modeling and drug development. In response to the global health emergency posed by the Zika virus (ZIKV) outbreak, brain organoids engineered to mimic the developing human fetal brain have been employed to model ZIKV-induced microcephaly. Here, we discuss the advantages of brain organoids over other model systems to study development and highlight recent advances in understanding ZIKV pathophysiology and its underlying pathogenesis mechanisms. We further discuss perspectives on overcoming limitations of current organoid systems for their future use in ZIKV research. |
| Author | Ming, Guo-li Nguyen, Ha Nam Qian, Xuyu Jacob, Fadi Song, Hongjun |
| AuthorAffiliation | 6 Department of Psychiatry and Behavioral Sciences , Johns Hopkins University School of Medicine , Baltimore, MD 21205 , USA 3 Department of Neurology , Johns Hopkins University School of Medicine , Baltimore, MD 21205 , USA 5 The Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine , Baltimore, MD 21205 , USA 4 The Solomon H. Snyder Department of Neuroscience , Johns Hopkins University School of Medicine , Baltimore, MD 21205 , USA 2 Biomedical Engineering Graduate Program, Johns Hopkins University School of Medicine , Baltimore, MD 21205 , USA 1 Institute for Cell Engineering, Johns Hopkins University School of Medicine , Baltimore, MD 21205 , USA |
| AuthorAffiliation_xml | – name: 1 Institute for Cell Engineering, Johns Hopkins University School of Medicine , Baltimore, MD 21205 , USA – name: 3 Department of Neurology , Johns Hopkins University School of Medicine , Baltimore, MD 21205 , USA – name: 6 Department of Psychiatry and Behavioral Sciences , Johns Hopkins University School of Medicine , Baltimore, MD 21205 , USA – name: 2 Biomedical Engineering Graduate Program, Johns Hopkins University School of Medicine , Baltimore, MD 21205 , USA – name: 4 The Solomon H. Snyder Department of Neuroscience , Johns Hopkins University School of Medicine , Baltimore, MD 21205 , USA – name: 5 The Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine , Baltimore, MD 21205 , USA |
| Author_xml | – sequence: 1 givenname: Xuyu surname: Qian fullname: Qian, Xuyu organization: Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA, Biomedical Engineering Graduate Program, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA – sequence: 2 givenname: Ha Nam surname: Nguyen fullname: Nguyen, Ha Nam organization: Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA, Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA – sequence: 3 givenname: Fadi surname: Jacob fullname: Jacob, Fadi organization: Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA, The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA – sequence: 4 givenname: Hongjun orcidid: 0000-0003-3307-421X surname: Song fullname: Song, Hongjun organization: Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA, Biomedical Engineering Graduate Program, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA, Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA, The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA, The Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA – sequence: 5 givenname: Guo-li orcidid: 0000-0002-2517-6075 surname: Ming fullname: Ming, Guo-li organization: Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA, Biomedical Engineering Graduate Program, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA, Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA, The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA, The Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/28292840$$D View this record in MEDLINE/PubMed |
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| Snippet | Technologies to differentiate human pluripotent stem cells into three-dimensional organized structures that resemble in vivo organs are pushing the frontiers... Technologies to differentiate human pluripotent stem cells into three-dimensional organized structures that resemble organs are pushing the frontiers of human... Technologies to differentiate human pluripotent stem cells into three-dimensional organized structures that resemble in vivo organs are pushing the frontiers... |
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| SubjectTerms | Animals Brain - pathology Drug development Fetuses Flavivirus Humans Microcephaly Microcephaly - pathology Microcephaly - virology Microencephaly Organoids Organoids - pathology Outbreaks Pathogenesis Pluripotency Spotlight Stem cells Zika virus Zika Virus - physiology Zika Virus Infection - virology |
| Title | Using brain organoids to understand Zika virus-induced microcephaly |
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