Paraburkholderia tagetis sp. nov., a novel species isolated from roots of Tagetes patula enhances the growth and yield of Solanum lycopersicum L. (tomato)

• Published in: Frontiers in microbiology Vol. 14; p. 1140484
• Main Authors: Chhetri, Geeta, Kim, Inhyup, Kim, Jiyoun, So, Yoonseop, Park, Sunho, Jung, Yonghee, Seo, Taegun
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 04.04.2023
• Subjects: Microbiology; Paraburkholderia; phosphate solubilization; plant growth promotion; polyhydroxybutyrate; Tagetes patula; tomato (Solanum lycopersicum L.); phosphate solubilization; Paraburkholderia; Tagetes patula; polyhydroxybutyrate; plant growth promotion; tomato (Solanum lycopersicum L.)
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2023.1140484

A multifunctional, Gram-stain-negative, aerobic, motile by flagella, short-rod shaped bacteria, designated strain RG36 T was isolated from roots of marigold plant ( Tagetes patula ) sampled at Dongguk University, Republic of Korea. A 16S rRNA sequences indicated that the closest phylogenetic neighbors were Paraburkholderia acidiphila 7Q-K02 T (99.0%) and Paraburkholderia sacchari IPT101 T (98.9%) of the family Burkholderiaceae. The draft genome size was 8.52 Mb (63.7% GC). The genome contained 7,381 coding sequences. Digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values of strain RG36 T with its most closely related species were only 83.1–88.7 and 27.6–36.7%, respectively. Strain RG36 T contained Q-8 as the major respiratory quinone and its main fatty acids (>10%) were C 16:0 , C 17:0 cyclo, C 19:0 cyclo ω8c, and summed feature 8 (comprising C 18:1 ω7c and/or C 18:1 ω6c). Strain RG36 T accumulates polyhydroxybutyrates (PHB) and exhibits multiple plant growth-promoting properties including production of indole-3-acetic acid (IAA), siderophores, protease, phosphate solubilization, and harboring gene clusters for its multifunctional properties. A pot experiment was conducted to evaluate the effect of PGPR on the growth of Solanum lycopersicum L. (Tomato). Result also confirmed the ability of strain RG36 T to promote tomato plant growth, especially it increases the yield of tomatoes. Structural assessment of the bioplastic by Fourier transform infrared (FTIR), nuclear magnetic resonance (NMR), and GC-MS spectroscopy, which confirmed the structure of the polymer as PHB. Our study revealed the potential of strain RG36 T to promote the growth of tomato plant and fruit yield by stimulating the various phytohormones, which could be use as bio-fertilizers to reduce the use of chemical fertilizers and promotes sustainable agricultural production. The phenotypic, chemotaxonomic and phylogenetic data, and genome analysis showed that strain RG36 T represents a novel species of the genus Paraburkholderia , for which the name Paraburkholderia tagetis sp. nov. is proposed. The type strain is RG36 T (=KACC 22685 T = TBRC 15696 T ).