Expanded Multilocus Sequence Typing for Burkholderia Species

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Published inJournal of Clinical Microbiology Vol. 47; no. 8; pp. 2607 - 2610
Main Authors Spilker, Theodore, Baldwin, Adam, Bumford, Amy, Dowson, Chris G., Mahenthiralingam, Eshwar, LiPuma, John J.
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.08.2009
American Society for Microbiology (ASM)
Subjects
Bacterial Typing Techniques - methods
Bacteriology
Biological and medical sciences
Burkholderia - classification
Burkholderia - genetics
Burkholderia cepacia
Cluster Analysis
DNA Fingerprinting - methods
DNA Primers - genetics
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
Fundamental and applied biological sciences. Psychology
Microbiology
Miscellaneous
Phylogeny
Sequence Analysis, DNA
Bacteria
Genotype
Burkholderia
Microbiology
Online AccessGet full text

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Abstract Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JCM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0095-1137 Online ISSN: 1098-660X Copyright © 2014 by the American Society for Microbiology.   For an alternate route to JCM .asm.org, visit: JCM       
AbstractList PCR primers targeting loci in the current Burkholderia cepacia complex multilocus sequence typing scheme were redesigned to (i) more reliably amplify these loci from B. cepacia complex species, (ii) amplify these same loci from additional Burkholderia species, and (iii) enable the use of a single primer set per locus for both amplification and DNA sequencing.PCR primers targeting loci in the current Burkholderia cepacia complex multilocus sequence typing scheme were redesigned to (i) more reliably amplify these loci from B. cepacia complex species, (ii) amplify these same loci from additional Burkholderia species, and (iii) enable the use of a single primer set per locus for both amplification and DNA sequencing.
PCR primers targeting loci in the current Burkholderia cepacia complex multilocus sequence typing scheme were redesigned to (i) more reliably amplify these loci from B . cepacia complex species, (ii) amplify these same loci from additional Burkholderia species, and (iii) enable the use of a single primer set per locus for both amplification and DNA sequencing.
Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JCM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0095-1137 Online ISSN: 1098-660X Copyright © 2014 by the American Society for Microbiology.   For an alternate route to JCM .asm.org, visit: JCM       
PCR primers targeting loci in the current Burkholderia cepacia complex multilocus sequence typing scheme were redesigned to (i) more reliably amplify these loci from B. cepacia complex species, (ii) amplify these same loci from additional Burkholderia species, and (iii) enable the use of a single primer set per locus for both amplification and DNA sequencing.
Author John J. LiPuma
Chris G. Dowson
Theodore Spilker
Amy Bumford
Adam Baldwin
Eshwar Mahenthiralingam
AuthorAffiliation Division of Infectious Diseases, Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan, 1 Department of Biological Sciences, Warwick University, Coventry, United Kingdom, 2 Cardiff School of Biosciences, Cardiff University, Cardiff, United Kingdom 3
AuthorAffiliation_xml – name: Division of Infectious Diseases, Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan, 1 Department of Biological Sciences, Warwick University, Coventry, United Kingdom, 2 Cardiff School of Biosciences, Cardiff University, Cardiff, United Kingdom 3
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  surname: Spilker
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  organization: Department of Biological Sciences, Warwick University, Coventry, United Kingdom
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  organization: Division of Infectious Diseases, Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan
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Corresponding author. Mailing address: Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, 8323 MSRB III, SPC 5646, 1150 W. Medical Center Drive, Ann Arbor, MI 48109-0646. Phone: (734) 936-9767. Fax: (734) 764-4279. E-mail: jlipuma@umich.edu
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Snippet Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley...
PCR primers targeting loci in the current Burkholderia cepacia complex multilocus sequence typing scheme were redesigned to (i) more reliably amplify these...
PCR primers targeting loci in the current Burkholderia cepacia complex multilocus sequence typing scheme were redesigned to (i) more reliably amplify these...
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StartPage 2607
SubjectTerms Bacterial Typing Techniques - methods
Bacteriology
Biological and medical sciences
Burkholderia - classification
Burkholderia - genetics
Burkholderia cepacia
Cluster Analysis
DNA Fingerprinting - methods
DNA Primers - genetics
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
Fundamental and applied biological sciences. Psychology
Microbiology
Miscellaneous
Phylogeny
Sequence Analysis, DNA
Title Expanded Multilocus Sequence Typing for Burkholderia Species
URI http://jcm.asm.org/content/47/8/2607.abstract
https://www.ncbi.nlm.nih.gov/pubmed/19494070
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Volume 47
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