Determination of the autophagic flux in murine and human peripheral blood mononuclear cells

• Published in: Frontiers in cell and developmental biology Vol. 11; p. 1122998
• Main Authors: Walter, Sophia, Jung, Tobias, Herpich, Catrin, Norman, Kristina, Pivovarova-Ramich, Olga, Ott, Christiane
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 13.03.2023
• Subjects: aging; cardiomyocytes; Cell and Developmental Biology; concanamycin A; LC3; lysosomes; NZO; cardiomyocytes; NZO; concanamycin A; sequestome-1; TFEB; lysosomes; aging; LC3

The autophagy lysosomal system (ALS) is crucial for cellular homeostasis, contributing to maintain whole body health and alterations are associated with diseases like cancer or cardiovascular diseases. For determining the autophagic flux, inhibition of lysosomal degradation is mandatory, highly comp...