A CRISPR-based approach using dead Cas9-sgRNA to detect SARS-CoV-2

• Published in: Frontiers in molecular biosciences Vol. 10; p. 1201347
• Main Authors: Aouida, Mustapha, Saifaldeen, Maryam, Al-Ansari, Dana E., Taleb, Sara, Hssain, Ali Ait, Ramotar, Dindial
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 14.06.2023
• Subjects: dead Cas9; diagnostics; M gene; Molecular Biosciences; restriction enzymes; RT-RPA; SARS-CoV-2; M gene; SARS-CoV-2; diagnostics; RT-RPA; dead Cas9; restriction enzymes

Rapid, highly specific, and robust diagnostic kits to detect viruses and pathogens are needed to control disease spread and transmission globally. Of the many different methods proposed to diagnose COVID-19 infection, CRISPR-based detection of nucleic acids tests are among the most prominent. Here,...