Acclimation of anaerobic sludge degrading chlorophenols and the biodegradation kinetics during acclimation period

• Published in: Chemosphere (Oxford) Vol. 54; no. 10; pp. 1573 - 1580
• Main Authors: Ye, Fen-xia, Shen, Dong-sheng
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.03.2004; Elsevier
• Subjects: Acclimation; Anaerobic sludge; Anaerobiosis; Applied sciences; Biodegradation, Environmental; Biological and medical sciences; Biological treatment of waters; Biotechnology; China; Chlorophenols; Chlorophenols - analysis; Chromatography, Gas; Chromatography, High Pressure Liquid; Dechlorination; Environment and pollution; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Industrial applications and implications. Economical aspects; Kinetics; Other wastewaters; Pollution; Refuse Disposal - methods; Sewage - chemistry; Sewage - microbiology; Sucrose; Time Factors; Wastewaters; Water treatment and pollution; Asia; China; Anaerobic sludge; Dechlorination; Chlorophenols; Kinetics; Acclimation; Biodegradation; Activated sludge; Biogas; Biological purification; Bioreactor; Surveillance; Anaerobe; Chemical oxygen demand; Chlorine Organic compounds; Batch process; Chemical composition; Urban waste water; Waste water purification; Organic compounds
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2003.08.019

The acclimation of sludge from Hangzhou citrate factory and Hangzhou municipal wastewater treatment plant for degradation dechlorination of chlorophenols (CPs) compounds, and its biodegradation kinetics were studied in batch process with or without addition of sucrose. Three monochlorophenols (2-CP; 3-CP; 4-CP) and pentachlorophenol (PCP) were concurrently fed to different bioreactors. The parameters that were monitored included biogas production, biogas composition and chemical oxygen demand (COD). The results showed that acclimation with chlorophenol can increase the degradation activity of anaerobic sludge and degradation rate of chlorophenolic compounds, and reduce the lag time. Degradation dechlorination activity of the acclimated sludge strongly depended on sludge source, microorganism population and chlorophenol congener. 2-CP was more easily acclimated than 3-CP and 4-CP. Among the four tested compounds, 4-CP was the most difficult to be acclimated. The observed degradation rate with presence of sucrose was higher than that with absence of sucrose, suggesting that addition of the external carbon source can stimulate the formation of acclimated sludge which could effectively degrade chlorophenols. Kinetic equations of biodegradation of chlorophenols were also presented in this paper.