Molecular Beacon Assay Development for Severe Acute Respiratory Syndrome Coronavirus 2 Detection

• Published in: Sensors (Basel, Switzerland) Vol. 21; no. 21; p. 7015
• Main Authors: Carvalho, Josué, Lopes-Nunes, Jéssica, Figueiredo, Joana, Santos, Tiago, Miranda, André, Riscado, Micaela, Sousa, Fani, Duarte, Ana Paula, Socorro, Sílvia, Tomaz, Cândida Teixeira, Felgueiras, Mafalda, Teixeira, Rui, Faria, Conceição, Cruz, Carla
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 22.10.2021; MDPI
• Subjects: Assaying; Bioinformatics; Coronaviruses; COVID-19; COVID-19 Testing; CRISPR; Design optimization; diagnosis; Disease transmission; Fluorescence; fluorescence detection; Genomes; Humans; Medical diagnosis; NMR; Nuclear magnetic resonance; Pandemics; Proteins; Respiratory diseases; RNA viruses; RNA, Viral; SARS-CoV-2; Sensitivity and Specificity; Severe acute respiratory syndrome coronavirus 2; Spectrum analysis; Thermal cycling; Viral diseases; Viruses; United States > US; COVID-19; RNA viruses; diagnosis; SARS-CoV-2; fluorescence detection

The fast spread of SARS-CoV-2 has led to a global pandemic, calling for fast and accurate assays to allow infection diagnosis and prevention of transmission. We aimed to develop a molecular beacon (MB)-based detection assay for SARS-CoV-2, designed to detect the ORF1ab and S genes, proposing a two-s...