A tool to automatically design multiplex PCR primer pairs for specific targets using diverse templates

• Published in: Scientific reports Vol. 13; no. 1; p. 16451
• Main Authors: Yang, Lin, Ding, Feng, Lin, Qiang, Xie, Junhua, Fan, Wei, Dai, Fangyin, Cui, Peng, Liu, Wanfei
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 30.09.2023; Nature Publishing Group; Nature Portfolio
• Subjects: 631/114; 631/208; 631/326; Adaptability; Agriculture; Automation; Conserved sequence; Datasets; Design; Entropy; Genes; Genomes; Genomics; Haplotypes; Heat shock proteins; Hsp65 protein; Humanities and Social Sciences; Identification; multidisciplinary; Nucleotide sequence; Phylogenetics; Phylogeny; rRNA 16S; Science; Science (multidisciplinary); Species diversity; Taxa

Multiplex PCR is an increasingly popular method for identifying species, investigating environmental diversity, and conducting phylogenetic analysis. The complexity and increasing availability of diverse templates necessitate a highly automated approach to design degenerate primer pairs for specific...