Altered Moesin and Actin Cytoskeleton Protein Rearrangements Affect Transendothelial Permeability in Human Endothelial Cells upon Dengue Virus Infection and TNF-α Treatment

• Published in: Viruses Vol. 13; no. 10; p. 2042
• Main Authors: Suttitheptumrong, Aroonroong, Mahutchariyakul, Thanaporn, Rawarak, Nantapon, Reamtong, Onrapak, Boonnak, Kobporn, Pattanakitsakul, Sa-Nga
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 11.10.2021; MDPI
• Subjects: Actin; Actin Cytoskeleton - metabolism; Actins - metabolism; Capillary Permeability; Chemokines; Cloning; Collagen; Cytokines; Cytoskeleton; Cytoskeleton - metabolism; cytoskeleton protein; Dengue - metabolism; Dengue fever; Dengue hemorrhagic fever; Dengue Virus - metabolism; dengue virus infection; Electrical resistivity; Endothelial cells; Endothelial Cells - virology; Flow cytometry; Humans; Immunofluorescence; Infections; Mass spectroscopy; Microfilament Proteins - metabolism; Moesin; Monoclonal antibodies; Permeability; Proteins; Proteomics; TNF-alpha; transendothelial permeability; Tumor Necrosis Factor-alpha - metabolism; Tumor necrosis factor-TNF; Tumor necrosis factor-α; Vinculin; Viral infections; United States > US; transendothelial permeability; dengue virus infection; TNF-alpha; cytoskeleton protein

It has been hypothesized that the host, viral factors, and secreted cytokines (especially TNF-α) play roles in the pathogenesis of secondary dengue infections. Mass spectrometry-based proteomic screening of cytoskeleton fractions isolated from human endothelial (EA.hy926) cells upon dengue virus (DE...