Metabolite Profiling of Hydroxycinnamate Derivatives in Plasma and Urine after the Ingestion of Coffee by Humans: Identification of Biomarkers of Coffee Consumption

• Published in: Drug metabolism and disposition Vol. 37; no. 8; pp. 1749 - 1758
• Main Authors: Stalmach, Angélique, Mullen, William, Barron, Denis, Uchida, Kenichi, Yokota, Takao, Cavin, Christophe, Steiling, Heike, Williamson, Gary, Crozier, Alan
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 01.08.2009; American Society for Pharmacology and Experimental Therapeutics
• Subjects: Beverages; Biological and medical sciences; Biomarkers - blood; Biomarkers - urine; Biotransformation; Caffeic Acids - blood; Caffeic Acids - urine; Chromatography, High Pressure Liquid; Cinnamates - blood; Cinnamates - pharmacokinetics; Cinnamates - urine; Coffee - metabolism; Coumaric Acids - blood; Coumaric Acids - pharmacokinetics; Coumaric Acids - urine; Glucuronates - blood; Glucuronates - urine; Humans; Hydroxylation; Medical sciences; Metabolomics - methods; Pharmacology. Drug treatments; Spectrometry, Mass, Electrospray Ionization; Sulfates - blood; Sulfates - urine; Consumption; Human; Urine; Biological fluid; Metabolite; Biological marker; Identification; Coffee; Ingestion; Blood plasma

Human subjects drank coffee containing 412 μmol of chlorogenic acids, and plasma and urine were collected 0 to 24 h after ingestion and were analyzed by high-performance liquid chromatography-mass spectrometry. Within 1 h, some of the components in the coffee reached nanomole peak plasma concentrati...