Highly accurate and sensitive absolute quantification of bacterial strains in human fecal samples

• Published in: Microbiome Vol. 12; no. 1; pp. 168 - 16
• Main Authors: Li, Fuyong, Liu, Junhong, Maldonado-Gómez, María X., Frese, Steven A., Gänzle, Michael G., Walter, Jens
• Format: Journal Article
• Language: English
• Published: England BioMed Central 07.09.2024; BMC
• Subjects: Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; ddPCR; DNA extraction; DNA, Bacterial - genetics; Feces - microbiology; Gastrointestinal Microbiome - genetics; High-Throughput Nucleotide Sequencing - methods; Humans; Lactobacillus; Limit of Detection; Limosilactobacillus reuteri; Limosilactobacillus reuteri - classification; Limosilactobacillus reuteri - genetics; Methodology; qPCR; Real-Time Polymerase Chain Reaction - methods; Reproducibility of Results; Sensitivity and Specificity; Strain-specific primers; ddPCR; DNA extraction; Strain-specific primers; qPCR; Limosilactobacillus reuteri; Lactobacillus

Next-generation sequencing (NGS) approaches have revolutionized gut microbiome research and can provide strain-level resolution, but these techniques have limitations in that they are only semi-quantitative, suffer from high detection limits, and generate data that is compositional. The present stud...