Highly accurate and sensitive absolute quantification of bacterial strains in human fecal samples - Li, Fuyong; Liu, Junhong; Maldonado-Gómez, María X.; Frese, Steven A.; Gänzle, Michael G.; Walter, Jens (Microbiome, 2024)
Keywords: Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; ddPCR; DNA extraction; DNA, Bacterial - genetics; Feces - microbiology; Gastrointestinal Microbiome - genetics; High-Throughput Nucleotide Sequencing - methods; Humans; Lactobacillus; Limit of Detection; Limosilactobacillus reuteri; Limosilactobacillus reuteri - classification; Limosilactobacillus reuteri - genetics; Methodology; qPCR; Real-Time Polymerase Chain Reaction - methods; Reproducibility of Results; Sensitivity and Specificity; Strain-specific primers; ddPCR; DNA extraction; Strain-specific primers; qPCR; Limosilactobacillus reuteri; Lactobacillus