Porcine Intestinal Organoids: Overview of the State of the Art

The intestinal tract is a crucial part of the body for growth and development, and its dysregulation can cause several diseases. The lack of appropriate in vitro models hampers the development of effective preventions and treatments against these intestinal tract diseases. Intestinal organoids are t...

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Published inViruses Vol. 14; no. 5; p. 1110
Main Authors Ma, Panpan, Fang, Puxian, Ren, Tianze, Fang, Liurong, Xiao, Shaobo
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 21.05.2022
MDPI
Subjects
Animal models
Animals
Biomedical research
Cell growth
Drug Discovery
Drug metabolism
drugs discovery
Genome editing
Hogs
host–microbe interactions
in vitro model
intestinal development
Intestine
Intestines
Kinases
Laboratory animals
Medical research
Metabolism
Organoids
Pathogens
Physiology
porcine intestinal organoids
Proteins
Review
Reviews
Stem cells
Swine
in vitro model
intestinal development
host–microbe interactions
porcine intestinal organoids
drugs discovery
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Abstract The intestinal tract is a crucial part of the body for growth and development, and its dysregulation can cause several diseases. The lack of appropriate in vitro models hampers the development of effective preventions and treatments against these intestinal tract diseases. Intestinal organoids are three-dimensional (3D) polarized structures composed of different types of cells capable of self-organization and self-renewal, resembling their organ of origin in architecture and function. Porcine intestinal organoids (PIOs) have been cultured and are used widely in agricultural, veterinary, and biomedical research. Based on the similarity of the genomic sequence, anatomic morphology, and drug metabolism with humans and the difficulty in obtaining healthy human tissue, PIOs are also considered ideal models relative to rodents. In this review, we summarize the current knowledge on PIOs, emphasizing their culturing, establishment and development, and applications in the study of host-microbe interactions, nutritional development, drug discovery, and gene editing potential.
AbstractList The intestinal tract is a crucial part of the body for growth and development, and its dysregulation can cause several diseases. The lack of appropriate in vitro models hampers the development of effective preventions and treatments against these intestinal tract diseases. Intestinal organoids are three-dimensional (3D) polarized structures composed of different types of cells capable of self-organization and self-renewal, resembling their organ of origin in architecture and function. Porcine intestinal organoids (PIOs) have been cultured and are used widely in agricultural, veterinary, and biomedical research. Based on the similarity of the genomic sequence, anatomic morphology, and drug metabolism with humans and the difficulty in obtaining healthy human tissue, PIOs are also considered ideal models relative to rodents. In this review, we summarize the current knowledge on PIOs, emphasizing their culturing, establishment and development, and applications in the study of host–microbe interactions, nutritional development, drug discovery, and gene editing potential.
The intestinal tract is a crucial part of the body for growth and development, and its dysregulation can cause several diseases. The lack of appropriate in vitro models hampers the development of effective preventions and treatments against these intestinal tract diseases. Intestinal organoids are three-dimensional (3D) polarized structures composed of different types of cells capable of self-organization and self-renewal, resembling their organ of origin in architecture and function. Porcine intestinal organoids (PIOs) have been cultured and are used widely in agricultural, veterinary, and biomedical research. Based on the similarity of the genomic sequence, anatomic morphology, and drug metabolism with humans and the difficulty in obtaining healthy human tissue, PIOs are also considered ideal models relative to rodents. In this review, we summarize the current knowledge on PIOs, emphasizing their culturing, establishment and development, and applications in the study of host-microbe interactions, nutritional development, drug discovery, and gene editing potential.The intestinal tract is a crucial part of the body for growth and development, and its dysregulation can cause several diseases. The lack of appropriate in vitro models hampers the development of effective preventions and treatments against these intestinal tract diseases. Intestinal organoids are three-dimensional (3D) polarized structures composed of different types of cells capable of self-organization and self-renewal, resembling their organ of origin in architecture and function. Porcine intestinal organoids (PIOs) have been cultured and are used widely in agricultural, veterinary, and biomedical research. Based on the similarity of the genomic sequence, anatomic morphology, and drug metabolism with humans and the difficulty in obtaining healthy human tissue, PIOs are also considered ideal models relative to rodents. In this review, we summarize the current knowledge on PIOs, emphasizing their culturing, establishment and development, and applications in the study of host-microbe interactions, nutritional development, drug discovery, and gene editing potential.
Author Xiao, Shaobo
Fang, Puxian
Fang, Liurong
Ma, Panpan
Ren, Tianze
AuthorAffiliation 2 The Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China
1 State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; panpma@163.com (P.M.); rtz199897@163.com (T.R.); fanglr@mail.hzau.edu.cn (L.F.); vet@mail.hzau.edu.cn (S.X.)
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Issue 5
Keywords in vitro model
intestinal development
host–microbe interactions
porcine intestinal organoids
drugs discovery
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Snippet The intestinal tract is a crucial part of the body for growth and development, and its dysregulation can cause several diseases. The lack of appropriate in...
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SubjectTerms Animal models
Animals
Biomedical research
Cell growth
Drug Discovery
Drug metabolism
drugs discovery
Genome editing
Hogs
host–microbe interactions
in vitro model
intestinal development
Intestine
Intestines
Kinases
Laboratory animals
Medical research
Metabolism
Organoids
Pathogens
Physiology
porcine intestinal organoids
Proteins
Review
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Stem cells
Swine
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Title Porcine Intestinal Organoids: Overview of the State of the Art
URI https://www.ncbi.nlm.nih.gov/pubmed/35632851
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Volume 14
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