Optimizing sheep B-cell epitopes in Echinococcus granulosus recombinant antigen P29 for vaccine development
is a widespread zoonotic parasitic disease, significantly impacting human health and livestock development; however, no vaccine is currently available for humans. Our preliminary studies indicate that recombinant antigen P29 (rEg.P29) is a promising candidate for vaccine. Sheep were immunized with r...
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| Published in | Frontiers in immunology Vol. 15; p. 1451538 |
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| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
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Frontiers Media S.A
14.08.2024
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| Abstract | is a widespread zoonotic parasitic disease, significantly impacting human health and livestock development; however, no vaccine is currently available for humans. Our preliminary studies indicate that recombinant antigen P29 (rEg.P29) is a promising candidate for vaccine.
Sheep were immunized with rEg.P29, and venous blood was collected at various time points. Serum was isolated, and the presence of specific antibodies was detected using ELISA. We designed and synthesized a total of 45 B cell monopeptides covering rEg.P29 using the overlap method. ELISA was employed to assess the serum antibodies of the immunized sheep for recognition of these overlapping peptides, leading to the preliminary identification of B cell epitopes. Utilizing these identified epitopes, new single peptides were designed, synthesized, and used to optimize and confirm B-cell epitopes.
rEg.P29 effectively induces a sustained antibody response in sheep, particularly characterized by high and stable levels of IgG. Eight B-cell epitopes of were identified, which were mainly distributed in three regions of rEg.P29. Finally, three B cell epitopes were identified and optimized: rEg.P29
, rEg.P29
, and rEg.P29
. These optimized epitopes were well recognized by antibodies in sheep and mice, and the efficacy of these three epitopes significantly increased when they were linked in tandem.
Three B-cell epitopes were identified and optimized, and the efficacy of these epitopes was significantly enhanced by tandem connection, which indicated the feasibility of tandem peptide vaccine research. This laid a solid foundation for the development of epitope peptide vaccine for
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| AbstractList | Echinococcus granulosus is a widespread zoonotic parasitic disease, significantly impacting human health and livestock development; however, no vaccine is currently available for humans. Our preliminary studies indicate that recombinant antigen P29 (rEg.P29) is a promising candidate for vaccine.BackgroundEchinococcus granulosus is a widespread zoonotic parasitic disease, significantly impacting human health and livestock development; however, no vaccine is currently available for humans. Our preliminary studies indicate that recombinant antigen P29 (rEg.P29) is a promising candidate for vaccine.Sheep were immunized with rEg.P29, and venous blood was collected at various time points. Serum was isolated, and the presence of specific antibodies was detected using ELISA. We designed and synthesized a total of 45 B cell monopeptides covering rEg.P29 using the overlap method. ELISA was employed to assess the serum antibodies of the immunized sheep for recognition of these overlapping peptides, leading to the preliminary identification of B cell epitopes. Utilizing these identified epitopes, new single peptides were designed, synthesized, and used to optimize and confirm B-cell epitopes.MethodsSheep were immunized with rEg.P29, and venous blood was collected at various time points. Serum was isolated, and the presence of specific antibodies was detected using ELISA. We designed and synthesized a total of 45 B cell monopeptides covering rEg.P29 using the overlap method. ELISA was employed to assess the serum antibodies of the immunized sheep for recognition of these overlapping peptides, leading to the preliminary identification of B cell epitopes. Utilizing these identified epitopes, new single peptides were designed, synthesized, and used to optimize and confirm B-cell epitopes.rEg.P29 effectively induces a sustained antibody response in sheep, particularly characterized by high and stable levels of IgG. Eight B-cell epitopes of were identified, which were mainly distributed in three regions of rEg.P29. Finally, three B cell epitopes were identified and optimized: rEg.P2971-90, rEg.P29151-175, and rEg.P29211-235. These optimized epitopes were well recognized by antibodies in sheep and mice, and the efficacy of these three epitopes significantly increased when they were linked in tandem.ResultsrEg.P29 effectively induces a sustained antibody response in sheep, particularly characterized by high and stable levels of IgG. Eight B-cell epitopes of were identified, which were mainly distributed in three regions of rEg.P29. Finally, three B cell epitopes were identified and optimized: rEg.P2971-90, rEg.P29151-175, and rEg.P29211-235. These optimized epitopes were well recognized by antibodies in sheep and mice, and the efficacy of these three epitopes significantly increased when they were linked in tandem.Three B-cell epitopes were identified and optimized, and the efficacy of these epitopes was significantly enhanced by tandem connection, which indicated the feasibility of tandem peptide vaccine research. This laid a solid foundation for the development of epitope peptide vaccine for Echinococcus granulosus.ConclusionThree B-cell epitopes were identified and optimized, and the efficacy of these epitopes was significantly enhanced by tandem connection, which indicated the feasibility of tandem peptide vaccine research. This laid a solid foundation for the development of epitope peptide vaccine for Echinococcus granulosus. is a widespread zoonotic parasitic disease, significantly impacting human health and livestock development; however, no vaccine is currently available for humans. Our preliminary studies indicate that recombinant antigen P29 (rEg.P29) is a promising candidate for vaccine. Sheep were immunized with rEg.P29, and venous blood was collected at various time points. Serum was isolated, and the presence of specific antibodies was detected using ELISA. We designed and synthesized a total of 45 B cell monopeptides covering rEg.P29 using the overlap method. ELISA was employed to assess the serum antibodies of the immunized sheep for recognition of these overlapping peptides, leading to the preliminary identification of B cell epitopes. Utilizing these identified epitopes, new single peptides were designed, synthesized, and used to optimize and confirm B-cell epitopes. rEg.P29 effectively induces a sustained antibody response in sheep, particularly characterized by high and stable levels of IgG. Eight B-cell epitopes of were identified, which were mainly distributed in three regions of rEg.P29. Finally, three B cell epitopes were identified and optimized: rEg.P29 , rEg.P29 , and rEg.P29 . These optimized epitopes were well recognized by antibodies in sheep and mice, and the efficacy of these three epitopes significantly increased when they were linked in tandem. Three B-cell epitopes were identified and optimized, and the efficacy of these epitopes was significantly enhanced by tandem connection, which indicated the feasibility of tandem peptide vaccine research. This laid a solid foundation for the development of epitope peptide vaccine for . BackgroundEchinococcus granulosus is a widespread zoonotic parasitic disease, significantly impacting human health and livestock development; however, no vaccine is currently available for humans. Our preliminary studies indicate that recombinant antigen P29 (rEg.P29) is a promising candidate for vaccine.MethodsSheep were immunized with rEg.P29, and venous blood was collected at various time points. Serum was isolated, and the presence of specific antibodies was detected using ELISA. We designed and synthesized a total of 45 B cell monopeptides covering rEg.P29 using the overlap method. ELISA was employed to assess the serum antibodies of the immunized sheep for recognition of these overlapping peptides, leading to the preliminary identification of B cell epitopes. Utilizing these identified epitopes, new single peptides were designed, synthesized, and used to optimize and confirm B-cell epitopes.ResultsrEg.P29 effectively induces a sustained antibody response in sheep, particularly characterized by high and stable levels of IgG. Eight B-cell epitopes of were identified, which were mainly distributed in three regions of rEg.P29. Finally, three B cell epitopes were identified and optimized: rEg.P2971-90, rEg.P29151-175, and rEg.P29211-235. These optimized epitopes were well recognized by antibodies in sheep and mice, and the efficacy of these three epitopes significantly increased when they were linked in tandem.ConclusionThree B-cell epitopes were identified and optimized, and the efficacy of these epitopes was significantly enhanced by tandem connection, which indicated the feasibility of tandem peptide vaccine research. This laid a solid foundation for the development of epitope peptide vaccine for Echinococcus granulosus. |
| Author | Yang, Jihui Lv, Yongxue Zhao, Wei Wu, Changyou Song, Jiahui Zhao, Yinqi Zhu, Mingxing Zhu, Yazhou Fu, Yong |
| AuthorAffiliation | 1 Center of Scientific Technology, Ningxia Medical University , Yinchuan , China 2 Ningxia Key Laboratory of Prevention and Treatment of Common Infectious Diseases, Ningxia Medical University , Yinchuan , China 4 Institute of Immunology, Zhongshan School of Medicine, Sun Yat-sen University , Guangzhou , China 5 Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University , Xining , China 3 School of Basic Medicine, Ningxia Medical University , Yinchuan , China |
| AuthorAffiliation_xml | – name: 3 School of Basic Medicine, Ningxia Medical University , Yinchuan , China – name: 4 Institute of Immunology, Zhongshan School of Medicine, Sun Yat-sen University , Guangzhou , China – name: 2 Ningxia Key Laboratory of Prevention and Treatment of Common Infectious Diseases, Ningxia Medical University , Yinchuan , China – name: 5 Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University , Xining , China – name: 1 Center of Scientific Technology, Ningxia Medical University , Yinchuan , China |
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| Cites_doi | 10.3389/fimmu.2018.01695 10.1186/s13071-021-04795-2 10.1016/j.biologicals.2023.101684 10.1016/j.vaccine.2016.10.064 10.3390/ijms241612931 10.7883/yoken.JJID.2017.412 10.3389/fimmu.2023.1086297 10.1186/s13071-020-4003-9 10.1016/j.ijbiomac.2023.128837 10.1111/j.1365-3024.2011.01341.x 10.1016/j.intimp.2024.111543 10.15171/bi.2018.06 10.3724/abbs.2022036 10.1016/j.rvsc.2020.11.010 10.3389/fcimb.2022.983119 10.3389/fimmu.2021.785293 10.1016/j.smim.2023.101725 10.1126/scitranslmed.adi1145 10.1093/abbs/gmn009 10.14411/fp.2013.004 10.1016/j.foodres.2020.109806 10.1016/j.vaccine.2015.05.065 10.1016/j.meegid.2019.04.017 10.1016/j.immuni.2022.07.020 10.1096/fj.202201636R 10.1007/s11259-016-9656-7 10.1371/journal.pntd.0004209 10.3389/fimmu.2023.1166924 10.1002/iid3.611 10.1016/j.autrev.2023.103340 10.3389/fimmu.2022.830497 10.7150/thno.93395 10.1016/j.actatropica.2021.106178 10.3389/fimmu.2021.668492 10.1017/s0031182099006186 10.14202/vetworld.2017.854-858 10.1016/j.clim.2012.01.015 10.1166/jbn.2021.3065 10.1016/j.vprsr.2020.100477 10.1128/CMR.00075-18 10.1111/zph.12649 10.4049/jimmunol.181.10.6679 |
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| Copyright | Copyright © 2024 Yang, Lv, Zhu, Song, Zhu, Wu, Fu, Zhao and Zhao. Copyright © 2024 Yang, Lv, Zhu, Song, Zhu, Wu, Fu, Zhao and Zhao 2024 Yang, Lv, Zhu, Song, Zhu, Wu, Fu, Zhao and Zhao |
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| Keywords | B cell epitopes recombinant antigen P29 vaccine sheep Echinococcus granulosus |
| Language | English |
| License | Copyright © 2024 Yang, Lv, Zhu, Song, Zhu, Wu, Fu, Zhao and Zhao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
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| Snippet | is a widespread zoonotic parasitic disease, significantly impacting human health and livestock development; however, no vaccine is currently available for... Echinococcus granulosus is a widespread zoonotic parasitic disease, significantly impacting human health and livestock development; however, no vaccine is... BackgroundEchinococcus granulosus is a widespread zoonotic parasitic disease, significantly impacting human health and livestock development; however, no... |
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| SubjectTerms | Animals Antibodies, Helminth - blood Antibodies, Helminth - immunology Antigens, Helminth - genetics Antigens, Helminth - immunology B cell epitopes Echinococcosis - immunology Echinococcosis - prevention & control Echinococcus granulosus Echinococcus granulosus - genetics Echinococcus granulosus - immunology Epitopes, B-Lymphocyte - immunology Immunology recombinant antigen P29 Recombinant Proteins - immunology Sheep Sheep Diseases - immunology Sheep Diseases - parasitology Sheep Diseases - prevention & control vaccine Vaccine Development Vaccines, Synthetic - immunology |
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| Title | Optimizing sheep B-cell epitopes in Echinococcus granulosus recombinant antigen P29 for vaccine development |
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