Production of tannase by Aspergillus niger Aa-20 in submerged and solid-state fermentation: influence of glucose and tannic acid

• Published in: Journal of industrial microbiology & biotechnology Vol. 26; no. 5; pp. 296 - 302
• Main Authors: Aguilar, C.N, Augur, C, Favela-Torres, E, Viniegra-Gonzalez, G
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.05.2001; Oxford University Press
• Subjects: Acids; Aspergillus niger; Aspergillus niger - drug effects; Aspergillus niger - enzymology; Aspergillus niger - growth & development; Aspergillus niger - metabolism; Biological and medical sciences; Biomass; Biotechnology; Carboxylic Ester Hydrolases - biosynthesis; Enzyme engineering; Enzymes; Fermentation; Fundamental and applied biological sciences. Psychology; Glucose; Glucose - metabolism; Hydrolyzable Tannins - metabolism; Hydrolyzable Tannins - pharmacology; Industrial Microbiology - methods; Kinetics; Methods. Procedures. Technologies; Microbiology; Nitrogen; Production of selected enzymes; solid state fermentation; Studies; tannase; Tannic acid; tannins; Enzyme; Esterases; Glucose; Carboxylic ester hydrolases; Fungi; Submerged culture; Aspergillus niger; Tannase; Production; Microorganism culture; Hydrolases; Solid substrate fermentation; Fungi Imperfecti; Thallophyta
• ISSN: 1367-5435; 1476-5535
• DOI: 10.1038/sj.jim.7000132

Tannase production by Aspergillus niger Aa-20 was studied in submerged (SmF) and solid-state (SSF) fermentation systems with different tannic acid and glucose concentrations. Tannase activity and productivity were at least 2.5 times higher in SSF than in SmF. Addition of high tannic acid concentrations increased total tannase activity in SSF, while in SmF it was decreased. In SmF, total tannase activity increased from 0.57 to 1.03 IU/mL, when the initial glucose concentration increased from 6.25 to 25 g/L, but a strong catabolite repression of tannase synthesis was observed in SmF when an initial glucose concentration of 50 g/L was used. In SSF, maximal values of total tannase activity decreased from 7.79 to 2.51 IU when the initial glucose concentration was increased from 6.25 to 200 g/L. Kinetic results on tannase production indicate that low tannase activity titers in SmF could be associated to an enzyme degradation process which is not present in SSF. Tannase titers produced by A. niger Aa-20 are fermentation system-dependent, favoring SSF over SmF.